Response of Penicillium Eu-0013 to different growth media for effective antibacterial compound(s) production

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Research Paper 01/03/2017
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Response of Penicillium Eu-0013 to different growth media for effective antibacterial compound(s) production

Jawad Anwar, Zafar Iqbal, Saima Roziman
Int. J. Biosci.10( 3), 124-131, March 2017.
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Penicllium Eu-0013 is seldom been studied in KPK-Pakistan against both agriculturally and medically important bacteria under different growth media treatments. As bacterial infection is a severe threat to agricultural products and individual healthiness hence the study was conducted to estimate the antibacterial potential of Penicillium Eu0013 under diverse developmental culture circumstances. Various soil born and endophytic fungi were isolated and preliminary screened for conventional antagonistic bactericidal effect against phytopathogenic bacteria Xanthomonas campestris and Clavibacter michiganensis followed by optimizing media constituents of the bioactive fungus for maximum antibacterial compounds production. Antibacterial tests using disc diffusion method and microdilution assays were carried out on the ethyl acetate and acetonitrile fraction of potent fungus against the two phytopathogenic bacteria and human pathogenic bacteria Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Penicillium Eu0013 was selected based on its antagonistic property. The Ethyl acetate extract of Penicillium Eu0013 obtained on Growth Nutrient Broth medium showed maximum zone of inhibition (23.3±0.264mm) against Clavibacter michiganensis followed by (19.366±0.351mm) against Xanthomonas campestris as compared to the zone of inhibition shown by extracts of other culture media (Glucose Peptone Yeast Broth, Potato Dextrose Broth and Yeast Extract Broth). Also the EtOAc extract and acetonitrile fraction stated the minimal value of MIC (5.208±2.255µg/ml and 10.416±4.510µg/ml) respectively against gram positive Staphylococcus aureus. It is revealed from the results that Penicillium Eu0013 is a potential source of novel antibiotic drugs as it showed a wide range of bioactivity against both phyto and human pathogenic bacteria. Different cultural conditions can yield different bioactive compounds.


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