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Response of Penicillium Eu-0013 to different growth media for effective antibacterial compound(s) production

Jawad Anwar, Zafar Iqbal, Saima Roziman

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Int. J. Biosci.10(3), 124-131, March 2017

DOI: http://dx.doi.org/10.12692/ijb/10.3.124-131


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Penicllium Eu-0013 is seldom been studied in KPK-Pakistan against both agriculturally and medically important bacteria under different growth media treatments. As bacterial infection is a severe threat to agricultural products and individual healthiness hence the study was conducted to estimate the antibacterial potential of Penicillium Eu0013 under diverse developmental culture circumstances. Various soil born and endophytic fungi were isolated and preliminary screened for conventional antagonistic bactericidal effect against phytopathogenic bacteria Xanthomonas campestris and Clavibacter michiganensis followed by optimizing media constituents of the bioactive fungus for maximum antibacterial compounds production. Antibacterial tests using disc diffusion method and microdilution assays were carried out on the ethyl acetate and acetonitrile fraction of potent fungus against the two phytopathogenic bacteria and human pathogenic bacteria Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Penicillium Eu0013 was selected based on its antagonistic property. The Ethyl acetate extract of Penicillium Eu0013 obtained on Growth Nutrient Broth medium showed maximum zone of inhibition (23.3±0.264mm) against Clavibacter michiganensis followed by (19.366±0.351mm) against Xanthomonas campestris as compared to the zone of inhibition shown by extracts of other culture media (Glucose Peptone Yeast Broth, Potato Dextrose Broth and Yeast Extract Broth). Also the EtOAc extract and acetonitrile fraction stated the minimal value of MIC (5.208±2.255µg/ml and 10.416±4.510µg/ml) respectively against gram positive Staphylococcus aureus. It is revealed from the results that Penicillium Eu0013 is a potential source of novel antibiotic drugs as it showed a wide range of bioactivity against both phyto and human pathogenic bacteria. Different cultural conditions can yield different bioactive compounds.


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Response of Penicillium Eu-0013 to different growth media for effective antibacterial compound(s) production

Ali A, Haider MS, Khokhar I, Bashir U, Mushtaq S, Mukhtar I. 2011. Antibacterial activity of culture extracts of penicillium species against soil-borne bacteria. Journal of Mycophytopathology 9, 17-20.

Aly AH, Debbab A, Proksch P. 2011. Fungal endophytes, unique plant inhabitants with great promises. Applied Microbiology and Biotechnology 90, 1829-1845. https://dx.doi.org/10.1007/s00253-011-3270-y.

Dobranic JK, Johnson JA, Alikhan QR. 1995. Isolation of endophytic fungi from eastern larch (Larix lancina) leaves from New Brunswick, Canada. Canadian Journal of Microbiology 41, 194-198. https://dx.doi.org/10.1139/m95-026

Domsch KH, Gams W, Anderson TH. 1980. Compendium of Soil Fungi. Academic press, London UK 1, 1-860. https://www.cabdirect.org/cabdirect/abstract/19811962574

Fenical W. 1993. Chemical studies of marine bacteria, developing a new resource. Chemical Reviews 93, 1673–1683.

Frisvad JC, Andersen B, Thrane U. 2008. The use of secondary metabolite profiling in chemotaxonomy of filamentous fungi. Mycological Research 112, 231–240. http://dx.doi.org/10.1016/j.mycres.2007.08.018

Gaddeyya G, Niharika PS, Bharathi P, Kumar PKR. 2012. Isolation and identification of soil mycoflora in different crop fields at Salur Mandal. Advances in Applied Science Research 3, 2020-2026.

Georgakopoulos DG, Fiddaman P, Leifert C, Malathrakis NE. 2002. Biological control of cucumber and sugar beet damping-off caused by Pythium ultimum with bacterial and fungal antagonists. Journal of Applied Microbiology 92, 1078–1086. https://dx.doi.org/10.1046/j.13652672.2002.01658.x

Giguère S. 2006. Antimicrobial Drug Action and Interaction.  Ames Iowa, USA.

Guo B, Wang Y, Sun X, Tang K. 2007. Bioactive Natural Products from Endophytes: A Review. Applied Biochemistry and Microbiology 44, 136-142. https://dx.doi.org/10.1134/S0003683808020026

Jain P, Pundir RK. 2011. Effect of fermentation medium, pH and temperature variations on antibacterial soil fungal metabolite production. Journal of Agricultural Technology 7, 247-269. https://www.cabdirect.org/cabdirect/abstract/20113327135

Reller LB, Melvin W, Jorgensen JH, Ferraro MJ. 2009. Antimicrobial susceptibility testing, a review of general principles and contemporary practices. Medical Microbiology 49, 1749-1755. https://dx.doi.org/10.1086/647952

Kerr RG, Kerr SS. 1999. Marine natural products as therapeutic agents.  Expert Opinion on Therapeutic patents 9, 1207-1222. https://dx.doi.org/10.1517/13543776.9.9.1207

Kloos D, Derks RJE, Wijtmans M, Lingeman H, Mayboroda OA, Deelder AM, Niessen WMA, Giera M. 2012. Derivatization of the tricarboxylic acid cycle intermediates and analysis by online solid-phase extraction-liquid chromatography–mass spectrometry with positive-ion electrospray ionization. Journal of Chromatography 3, 19–26. https://dx.doi.org/10.1016/j.chroma.2011.07.095

Kuete V. 2010. Potential of Cameroonian plants and derived products against microbial infections: A review. Plant medica 76, 1479-1491. https://dx.doi.org/10.1055/s-0030-1250027

Lindhagen E, Nygren P, Larsson R. 2008. The fluorometric microculture cytotoxicity assay. Nature protocols 3, 1364-1369. https://dx.doi.org/10.1038/nprot.2008.114

Petrini O, Sieber T, Toti L, Viret O. 1992. Ecology metabolite production and substrate utilization in endophytic fungi. Natural Toxins 1, 185-196. https://dx.doi.org/10.1002/nt.2620010306

Rajasekar T, Balaji S, Kumaran S. 2012. Isolation and characterization of marine fungi metabolites against clinical pathogens. Asian Pacific Journal of Tropical Disease 4, 387-392. http://dx.doi.org/10.1016/S2222-1808(12)60187-X

Ramasamy K, Lim SM, Bakar AB, Ismail N, Ismail MS, Ali MF, Weber JFF, Cole ALJ. 2010. Antimicrobial and cytotoxic activities of Malaysian endophytes. Phytotherapy Research 24, 640-643. https://dx.doi.org/10.1002/ptr.2891

Ramos HP, Said S. 2011. Modulation of biological activities produced by an endophytic fungus under different culture conditions. Advances in Bioscience and Biotechnology 2, 443-449. https://dx.doi.org/10.4236/abb.2011.26065

Roger MJR, Reigosa MJ, Pedrol N, González L. 2006. Allelopathy, a physiological process with ecological implications. Springer 637.

Slininger PJ, Shea-Wilbur MA. 1995. Liquid culture pH, temperature carbon and nitrogen source regulate phenazine productivity of the take all biocontrol agent pseudomonas fluorescence. Applied Microbiology and Biotechnology 37, 388-392. https://dx.doi.org/10.1007/BF02431910

Strobel GA. 2003. Endophytes as sources of bioactive products. Microbes and Infectection 5, 535-544. http://dx.doi.org/10.1016/S1286-4579(03)00073-X

Sundstrom C, Nilsson K. 1976. Establishment and characterization of a human histiocytic lymphoma cell line (U-937). International Journal of Cancer 17, 565-577. https://dx.doi.org/10.1002/ijc.2910170504

Tong WY, Darah I, Latifah Z. 2011. Antimicrobial activities of endophytic fungal isolates from medicinal herb Orthosiphonstamineus Benth. Journal of Medicinal Plants Research 5, 831-836. https://dx.doi.org/10.5897/JMPR

Vijaykumar SJ, Sasidharannair NK, Nambision B, Mohandas C. 2013. Optimization of media and temperature for enhanced antimicrobial production by bacteria associated with Rhabitis sp. Iranian Journal of microbiology 5, 136-141.

Yu H, Zhang L, Li LZ, Guo C, Li L, Sun W, Qin L. 2010. Recent developments and future prospects of antimicrobial metabolites produced by endophytes. Microbiological Research 165, 437-449. http://dx.doi.org/10.1016/j.micres.2009.11.009

Zain ME, Awaad AS, Al-Jaber NA, Maitland DJ. 2008. New phenolic compounds with antifungal activity from Aspergillus terreus isolated from desert soil. Journal of Saudi Chemical Society 12, 107-114.


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