home icon user icon
Welcome to International Network for Natural Sciences | INNS Pub.
Home My Account
Submit Manuscript
double_slash
breadcumb_bg

Salicylic acid affects antioxidant system of some grape cultivar under cold stress conditions

Paper Details

Research Paper 01/11/2014
Views (364) Download (11)
current_issue_feature_image
publication_file

Salicylic acid affects antioxidant system of some grape cultivar under cold stress conditions

Mohammad Ali Aazami, Nasser Mahna, Rahim Naghshband Hasani
J. Bio. Env. Sci.5( 5), 280-290, November 2014.
Certificate: JBES 2014 [Generate Certificate]
Key: Antioxidant systemCold stressGrapevineSalicylic acid

Abstract

Salicylic acid (SA) is a plant growth regulator mediates and ameliorates damages caused by cold stress in plant cells. To investigate the effects of SA foliar spray on soluble proteins content, antioxidants enzymes activity catalase (CAT), ascorbate peroxidase (APX), Guaiacol peroxidase (GPX), superoxide dismutase (SOD), Glutathion reductase (GR) and the effects on the amounts of hydrogen peroxide (H2O2) and malondialdehyde (MDA) in two grapevine cultivars ٬Thompson seedless٫ and ٬Gizil uzum٫ an experiment was conducted with 4 SA level (Control and foliar application 1, 6 and 12 hrs before the cold stress treatment) and 5 thermal levels (Control of 22 ºC and for 1, 3, 6 and 12 hrs subjection to 1ºC as cold treatments) factorial based on CRD with 4 replications under controlled growing condition. The results revealed that with the increase in cold stress subjection, the activity of APX, SOD and GR was decreased. In line, GPX activity with ٬Gizil uzum٫ was increased with the increase in cold subjection treatment. CAT activity in ٬Gizil uzum٫ was increased with cold treatment as well and the highest CAT activity was recorded with 3 hrs cold treatment exposure. SA treatment led to the amended activity of CAT enzyme in ٬Gizil uzum٫ and also the increased activity of APX, SOD and GR in both the cultivars. Otherwise, SA treatment had no significant effect on the activity of GPX. SA treatment (12 hrs before than the cold treatment) led to a reasonable reduction in MDA content. Finally, SA due to its ameliorative effects and the induction of acquired tolerance. Improved the antioxidant enzymes activity and also increased the cold stress tolerance with the studied cultivars and especially with ٬Gizil uzum٫.

VIEWS 24
Cover PDF

Aebi H. 1984. Catalase in vitro. Methods Enzymology 105, 121-126.

Belintani NG, Guerzoni JTS, Moreira RMP, Vieira LGE. 2012. Improving low –temperature tolerance in sugarcane by expressing the ipt gene under a cold inducible promoter. Biol. Plant. 56, 71-77.

Bracale M, Coraggio I. 2003. Chilling and freezing stresses in plants: cellular responses and molecular strategies for adaptation. Abiotic stresses in plants. Boston, Kluwer Academic publishers, 23-51.

Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Annals of Biochemistry 7, 248-254.

Chakraborty U, Tongden C. 2005. Evolution of heat acclimation and salicylic acid treatment as potent inducers of thermotolerance in Cicer arietinum L. Curr. Sci. 89, 384-389.

Chaves MM, Oliveira MM. 2004. Mechanisms underlying plant resilience to water deficits: prospects for water-saving agriculture. Journal of Experimental Botany 55, 2365-2384.

Chen S, Zimei L, Cui J, Jiangang D, Xia X, Liu D, Yu J. 2011. Alleviation of chilling induced oxidative damage by salicylic acid pre-treatment and related gene expression in eggplant seedlings. Plant growth Regul. 65, 101-108.

Ding CK, Wang CY, Gross KC, Smith DL. 2014. Jasmonate and salicylate induce the expression of pathogenesis related protein genes and increase resistance to chilling injury in tomato fruit. Planta. 214, 895-901.

FAOSTATdata .2012. http://faostat.fao.org

Fennell A. 2004. Freezing tolerance and injury in grapevines. Journal of Crop Improvement 10, 201-235.

Feng H, Li X, Duan J, Li X, Liang H. 2008. Chilling tolerance of wheat seedlings is related to an enhanced alternative respiratory pathway. Crop Sci. 48, 2381-2388.

Ferrandino A, Lovisolo C. 2014. Abiotic stress effects on grapevine (Vitis vinifera L.): Focus on abscisic acid mediated consequences on secondary metabolism and berry quality. Environmental and Experimental Botany 103, 138-147.

Gramer GR, Lauchli A. 1986.Ion activities in solution in relation to Na2+– Ca2+ intraction at the plasmalemma. J. of Exp. Bot. 37, 321-330.

Guy C. 1999. Molecular responses of plants to cold shock and cold acclimation. Journal of Molecular and Microbial Biotechnology 1, 231-242.

Hayat S, Ahmad A. 2007. Salicylic acid: A plant hormone. Springer, Dordrecht.

Hernandez JA, Jimenez A, Mullineauxs PM, Sevilla F. 2000. Tolerance of Pea (Pisum sativum L.) to long-term salt stress is associated with induction of antioxidant defenses. Plant cell Environ. 23, 853-862.

Hola D, Kocova M, Rothova O, Wilhelmova N, Benesova M. 2007. Recovery of maize (Zea mays L.) inbreds and hybrids from chilling stress of various duration: Photosynthesis and antioxidant enzymes. Journal of Plant Physiology 164, 868-877.

Horvath E, Janada T, Szalia G, Paldi E. 2002. In vitro salicylic acid inhibition of catalase activity in maize: differences between the isozymes and a possible role in the induction of chilling tolerance. Plant Sci. 163, 1129-1135.

Janda T, Szalai G, Tari I Paldi E. 1999. Hydroponic treatment with salicylic acid decreases the effect of chilling injury (Zea mays L.) plants. Planta. 208, 175-180.

Janda T, Szalai G, Rios-Ganzalez K, Veisz O, Paldi E. 2003. Comparative study of frost tolerance and antioxidant activity in cereals. Plant Sci. 164, 301-306.

Janda T, Kosa EL, Szalai G, Paldi E. 2005. Investigation of antioxidant activity of maize during low temperature stress. Journal of Plant Physiology 49, 43-54.

Kang G, Wang C, Sun G, Wang Z. 2003. Salicylic acid changes activities of H2O2 metabolizing enzymes and increase the chilling tolerance of banana seedlings. Environ. Exp. Bot. 50, 9-15.

Li W, Ling WY, Kang L. 2005. Effect of exogenous salicylic acid on the cold resistance of the red globe grape. J. Xinjiang. Agr. Univ. 28, 51-54.

Limone-Pacheco J, Gonsebatt ME. 2009. The role of antioxidants and antioxidant-related enzymes in protective responses to environmentally induced oxidative stress. Genetic Toxicology and environmental Mutagenesis 674, 137-147.

Israr M, Sahi SV, Jain J. 2006. Cadmium accumulation and antioxidative responses in the Sesbania drummondii callus. Arch. Environ. Contam. Toxicol. 50, 121-127.

Mahajan S, Tuteja N. 2005. Cold, Salinity and drought stresses: an overview. Archives of Biochemistry and Biophysics 444, 139-158.

Mallik S, Nayak M, Sahu BB, Panigrahi AK, Shaw BP. 2011. Response of antioxidant enzymes to high NaCl concentration in different salt-tolerant plant. Biol. Plant. 55, 191-195.

Mills LJ, Ferguson JC, Keller M. 2006. Cold hardiness evolution of grapevine buds and cane tissues. Am. J. Enol. Vitic. 57, 194-200.

Mittler R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends of Plant Sciences 7, 405-410.

Miura K, Furumoto T. 2013. Cold signalling and cold response in plants. Int. J. Mol. Sci. 14, 5312-5337.

Miura K, Tada Y. 2014. Regulation of water, salinity, and cold stress responses by salicylic acid. Frontiers in Plant Science. 5, 1-12.

Morsy MR, Jouveb J, Hausmanb F, Hoffmannb L, Stewart M. 2006. Alteration of oxidative and carbohydrate metabolism under abiotic stress in two rice (Oryza Sativa L.) genotypes contrasting in chilling tolerance. Journal of Plant Physiology 21, 325-332.

Mutlu S, Atici O, Nalbantoglu B. 2009. Effects of salicylic acid and salinity on apoplastic antioxidant enzymes in two weat cultivars differing in salt tolerance. Biol. Plant. 53, 334-338.

Mutlu S, Kavadagoglu O, Atici O. 2013. Protective role of salicylic acid applied befor cold stress on antioxidative system and protein patterns in barley apoplast. Biologia Plantarum. 57, 507-513.

Patykowski J, Urbanek H. 2003. Activity of enzymes related to H2O2 generation and metabolism in leaf apoplastic fraction of tomato leaves in feted with Botrytis cinerea. J. Phytopathol. 151, 153-161.

Sairam RK, Rao KV, Srivastava GC. 2002. Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Science 163, 1037-1046.

Scandalios JG. 1993. Oxygen stress and superoxide dismutase. Plant Physiology. 101, 7-12.

Senaratna T, Touchell D, Bunn E, Dixon K. 2000. Acetyl salicylic acid (aspirin) and salicylic acid induce multiple stress tolerance in bean and tomato. Plant Growth Regul. 30, 157-161.

Sen Gupta A, Webb RP, Holaday A, Sallen RD. 1993. Overexpression of superoxide dismutase protects plants from oxidative stress. Plant Physiology 103, 1067-1073.

Sergiev I, Alexieva V, Karanov E. 1997. Effect of spermine, alrazine and combination between them on some endogenous protective systems and stress markers in plant. Comptes Rendus. Acadmic Blugare Sciences. 51, 121-124.

Sharma P, Sharma N, Deswal R. 2005. The Molecular biology of the low-temperature response in plants. Bioassays 27, 1048-1059.

Soumen B. 2005. Reactive oxygen species and oxidative burst roles in stress, senescence and signal transduction in plants. Current Science 89, 1113-1121.

Stewart RRC, Bewley JD. 1980. Lipid peroxidation associated aging of soybean axes. Plant Physiol. 65, 245-248.

Tasgin E, Atici O, Nalbantoglu B, Popova LP. 2006. Effects of salicylic acid and cold treatments on protein levels and on the activities of an antioxidant enzymes in the apoplast of winter wheat leaves. Phytochemistry 67, 710-715.

Turhan E, Karni L, Aktas H, Deventurero G, Chang D C, Bar-Tal A, Aloni B. 2006. Apoplastic antioxidants in pepper (Capsicum annuum L.) fruit and their relationship to blossom-end rot. J. Hort. Sci. Biotechnol. 81, 661-667.

Wang DH, Li XX, Su ZK, Ren HX. 2009. The role of salicylic acid in response of two rice cultivars to chilling stress. Biol. Plant. 53, 545-552.

Wang WB, Kim YH, Lee HS, Yong Kim K, Deng X, Kwak S. 2009. Analysis of antioxidant enzyme activity during germination of alfalfa under salt and drought stresses. Journal of Plant Physiology and Biochemistry 47, 570-577.

Wathugala D. 2012. The role of plant mediator as a gene regulator to tackle climate change: A review. Journal of Environmental Professionals Sri Lanka 1, 43-56.

Wilkinson S, Clephan AL, Davies WJ. 2001. Rapid low temperature induced stomatal closure occurs in cold tolerant Commelina communis leaves but not in cold sensitive tobacco leaves, via a mechanism that involves apoplastic calcium but not abscisic acid. Plant Physiology 126, 1566-1578.

Yoshimura K, Yabute Y, Ishikawa T, Shigeoka S. 2000. Expression of spinach ascorbate per-oxidase isoenzymes in response to oxidative stresses. Plant Physiology 123, 223-233.

Yuan S, Lin HH. 2008. Role of salicylic acid in plant abiotic stress. Z. Naturoforsch. 63, 313-320.

Zhang Q, Zhang JZ, Chow WS, Sun LL, Chen J W, Chen YJ, Peng CL. 2011. The influence of low temperature on photosynthesis and antioxidant enzymes in sensitive banana and tolerant plantain (Musa sp.) cultivars. Photosyntetica. 49, 201-208.

Zhou BY, Guo ZF, Liu ZL. 2005. Effects of abscisic acid on antioxidant systems of Stylosanthes guianensis (Aublet) Sw. under chilling stress. Crop Science 45, 599-605.

Submit Manuscript