J. Bio. Env. Sci.5(5), 280-290, November 2014
Salicylic acid (SA) is a plant growth regulator mediates and ameliorates damages caused by cold stress in plant cells. To investigate the effects of SA foliar spray on soluble proteins content, antioxidants enzymes activity catalase (CAT), ascorbate peroxidase (APX), Guaiacol peroxidase (GPX), superoxide dismutase (SOD), Glutathion reductase (GR) and the effects on the amounts of hydrogen peroxide (H2O2) and malondialdehyde (MDA) in two grapevine cultivars ٬Thompson seedless٫ and ٬Gizil uzum٫ an experiment was conducted with 4 SA level (Control and foliar application 1, 6 and 12 hrs before the cold stress treatment) and 5 thermal levels (Control of 22 ºC and for 1, 3, 6 and 12 hrs subjection to 1ºC as cold treatments) factorial based on CRD with 4 replications under controlled growing condition. The results revealed that with the increase in cold stress subjection, the activity of APX, SOD and GR was decreased. In line, GPX activity with ٬Gizil uzum٫ was increased with the increase in cold subjection treatment. CAT activity in ٬Gizil uzum٫ was increased with cold treatment as well and the highest CAT activity was recorded with 3 hrs cold treatment exposure. SA treatment led to the amended activity of CAT enzyme in ٬Gizil uzum٫ and also the increased activity of APX, SOD and GR in both the cultivars. Otherwise, SA treatment had no significant effect on the activity of GPX. SA treatment (12 hrs before than the cold treatment) led to a reasonable reduction in MDA content. Finally, SA due to its ameliorative effects and the induction of acquired tolerance. Improved the antioxidant enzymes activity and also increased the cold stress tolerance with the studied cultivars and especially with ٬Gizil uzum٫.
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