home icon user icon
Welcome to International Network for Natural Sciences | INNS Pub.
Home My Account
Submit Manuscript
double_slash
breadcumb_bg

Screening and characterization of amylase producing bacteria from soil contaminated with cassava effluents from Abraka, Delta State

Paper Details

Research Paper 01/02/2022
Views (753) Download (88)
current_issue_feature_image
publication_file

Screening and characterization of amylase producing bacteria from soil contaminated with cassava effluents from Abraka, Delta State

Adomi O Patience
J. Bio. Env. Sci.20( 2), 42-47, February 2022.
Certificate: JBES 2022 [Generate Certificate]
Key: Amylase producing bacteriaCharacterizationSoil

Abstract

Amylases are starch hydrolyzing enzymes important in normal daily life and industry. There are different sources of amylase production however, enzymes derived from microorganisms are cheaper and thermos table among other features. The aim of this work was to isolate amylase producing bacteria from soil contaminated with cassava water effluents and to find out environmental/ optimization of amylase production. Soil samples were collected from four different sites. A total of 50 bacterial were isolated form soil samples, 14 showed area of clear zones on starch medium. Isolates that gave maximum yield of amylase were identified molecularly. The isolates were optimized for carbon sources, pH, nitrogen sources, incubation time, agro waste and chlorides. Biochemical and molecular analysis showed that Citrobacter sp and Enterobacter cloaca showed higher amylase activity. Glucose, nitrate, pH 7.0, magnesium chloride and temperature of 25oC produced maximum amylase. Plantain peel was the agro water that produced more amylase enzyme.

VIEWS 194
Cover PDF

Bole S, Maji A, Dey A, Acharya A, Dubey, Rakeshlal S. 2013. Isolation, purification and characterization of amylase fron airborne bacteria World Journal of Pharmacy& Pharmceutical Sciences 2(5), 228-246.  www.wippo.com

Ekka A, Namdeo N. 2018 Screening, Isolation and Characeterization of amylase production bacteria and optimization for production od amylase IOSR Journal of Biotechnology and Biochemistry 4(2), 50-56.

Fasiku SA, Ogunsola OF, Fakunle A, Olanbiwoninu AA. 2020. Isolation of Bacteria with potential of producing extracellular enyzmes (Amylase, cellulose and protease) from soil. Journal of Advances in Microbiology 21-26.

Fossi BT, Taveand F, Ndjonenkeu T. 2005. Production and partial characterization of a thermostable amylase from ascomycetes yeast strain isolated from starchy soils. African Journal of Biotechnology 4(1), 14-18.

Geesha. 2016. https://pediaa.com/difference-between -alpha-and-beta-amylase/ accessed 2nd November 2021.

Gupta A, Gupta VK, Modi DR, Yadawa LP. 2008. Production and characterization of amylase for Aspergillus niger. Biotechnology 7(3), 551-556.

Oluwa damilare LA, Oluwatofunmi OE, Dzorbenya AG, Farinn AO. 2019. A review of literature on isolation of bacteria alpha amylase, International Research Journal of Engineering and Technology (IRJET) 6(1), 1333-1342.

Orji JC, Nweke CO, Nwabueze RN, Nwanyanwu EN, Alisi CS, Etim-Osowo EN. 2009. Production and properties of a-amylase from Citrobacter species. Revista Ambiente &Agua. An Interdisciplinary journal of Applied Scien 4(1), 45-56.

Oseni AO. Ekperigin MM. 2013. Isolation and activity of alpha amylase from selected bacteria strains in the forest soil. GJOB 2(1), 12-20.

Pokhrel B, Wanjare P, Singh S, Purushotham B. 2013. “Isolation, screening and characterization of promising-amylase producing bacteria from sewage enriched soil”, IJABR (4), 286-290.

Saini R, Saini HS, Dahiya A. 2017. Amylases: Characterization and Industrial application Journal of Pharmacognosy and Phytochemistry 6(4), 1865-1871.

Sexena R, Singh R. 2011. Amylase production by solid state fermentation of Agro-Industrial waste using Bacillus species BTM 42, 1334-1342.

Swetha S, Gangadharon D. 2006 A- amylase from Microbial Sources- An Overview recent development Food Technol Biotechnol 44(2), 173-184.

Swetha Sivaramakrishnan, Gangadharon Dhanya. 2006. α- Amylases from Microbial Sources–An Overview on Recent Developments. Food Technol Biotechnol. March 44(2), 173-184.

Validya S, Rathore P. 2015. Isolation, screening and characterization of amylase producing bacteria for soil of potato dump sites from different regions of Madhya Pradish http://www.researchgate.net /publication /277242517

Veerapagu M, Yeeya KR, Sankaranarayaran A. 2016. Screening and production of fungal amylase from Aspergillus sp by SSF. Journal of Global Biosciences 5(8), 4443-4450.

Verma V, Avasthi S, Singh M. 2011. Amylase production and purification from bacteria isolated from waste potato dumpsite. European Journal of Experimental Biology 1, 107-113.

Submit Manuscript