Screening and evaluation of ligninolytic dye decolourisation capacity of Pleurotus ostreatus
Paper Details
Screening and evaluation of ligninolytic dye decolourisation capacity of Pleurotus ostreatus
Abstract
Ligninolytic enzymes as biocatalysts have the potential to replace conventional processes in several industries including the water purification industry especially in the treatment of dye effluent. Development of water treatment systems based on ligninolytic enzymes is a desirable option because ligninolytic enzymes can degrade dyes of diverse chemical structure and be used in a wide variety of industries. Studies were carried out to evaluate and screen for ligninolytic dye decolourisation capacity of Pleurotus ostreatus (P.ostreatus). Pure fungal cultures of P.ostreatus were screened for ligninolytic enzyme activity using solid phase decolourisation of aromatic food dyes. The assay was carried out on PDA plates with 100mg/l of individual commercial food dyes – Sunset Yellow, Orange C10 and Lemon Yellow. All the food dyes were effectively bleached or decolourised by fungal mycelia of P.ostreatus after 10 days of active growth in the dark at 25oC. The observation strongly suggests the presence of fungal peroxidase enzymes which play a role in the degradation of synthetic lignin or dyes. Lignin peroxidase (LiP) activity from crude enzyme extract was determined using the method of Tien and Kirk, 1988. An average LiP activity of 7.635U/ml was observed under Solid State Fermentation (SSF) by P.ostreatus in wheat bran/soya bean substrate (90:10) after 10 days of full substrate colonisation. In agreement with the results of other workers, the study indicates potential for P.ostreatus for industrial production of ligninolytic enzymes through solid state fermentation on locally available agricultural products.
Abdulla E, Tzanov T, Costa S, Robra KH, Cavaco-Paulo A, Gübitz GM. 2000. Decolourization and detoxification of textile dyes with a laccase from Trametes hirsuta. Applied and Environmental Microbiology 66, no. 8, 3357-3362.
Ahlawat OP, Gupta P, Raj D, Vijay B. 2006. Dye decolourization potential of spent substrates from Agaricus bisporus and Pleurotus sp. -a laboratory study. Mushroom. Research 15(1), 75-82.
Alhassani HA, Muhammad RA, Ashraf SS. 2007. Efficient Microbial Degradation of toluidine Blue dye by Brevibacillus sp. Dyes and Pigments 755, 395-400.
Anliker R. 1979. Eco-toxicological assessment of dyes with particular reference to ETAD’s activities, Journal of the Society of Dyers and Colourists 95, p 317–326.
Azmi W, Sani RK, Banerjee UC. 1998. Biodegradation of triphenylmethane dyes. Enzyme and Microbial Technology 22, 185–191.
Banat IM, Nigam P, Singh D, Marchant R. 1996. Microbial decolourization of textile dye-containing effluents: a review, Bio Resource Technology 58,217–227.
Carliell CM, Barclay SJ, Naidoo N, Buckley CA, Mulholland DA, Senior E. 1995. Microbial decolourization of a reactive azo dye under anaerobic conditions. Water South Africa 21, 61-69. 11.
Chang JS, Chou C, Lin Y, Ho JY, Hu TL. 2001. Kinetic characteristics of bacterial azo-dye decolourization by Pseudomonas luteola. Water Research 35, 2841-2850.
Cho NS, Wilkolazka AJ, Staszczak M, Cho HY, Ohga S. 2009. The role of laccase from white rot fungi to stress conditions. Journal of the Faculty of Agriculture, Kyushu University 54, no. 1, 81-83.
Dhouib A, Hamza M, Zouari H, Mechichi T, H’midi R, Labat M, Martínez MJ, Sayadi S. 2005. Autochthonous fungal strains with high ligninolytic activities from Tunisian biotopes. African Journal of Biotechnology 4, 431-436.
Dzulkalfi A, Au F. 2012. Food Dyes Decolourized by Pleurotus ostreatus. International Journal of Scientific and Research Publications Volume 2, Issue 4, April 2012 1 ISSN 2250-3153.
Eichlerová I, Homolka L, Benada O, Kofronová O, Hubálek T, Neruda F. 2007. Decolourization of Orange G and Remazol Brilliant Blue R by the white rot fungus Dichomitus squalens; Toxicological evaluation and morphological study. Chemosphere 69, 795-802.
Erkurt EA, Ali UN, Kumbur H. 2007. Decolourization of synthetic dyes by white rot fungi, involving laccase enzyme in the process. Process Biochemistry 42, 1429-1435.
Ferdinandi P, Godliving YSM, Anthony MM, Amelia KK. 2014. Ligninolytic enzymes activities of Pleurotus Ostreatus P969 during vegetative growth and fruit development on sugarcane residues-based substrates. The International Journal of Biotechnology 3(4), 58-71.
Fu Y, Tiraraghavan Y. 2001. Fungal decolourization of dye waste waters: a review, Bio resource Technology 79, 251-262.
Gharbani P, Tabatabaii SM, Mehrizad A. 2008. Removal of Congo red from textile wastewater by ozonation. International Journal of Environmental Science of Technology 5 (4), 495-500.
Ghasemi F, Tabandeh F, Bambai B, Sambasiva KRS. 2010. Decolourization of different azo dyes by Phanerochaete chrysosporium RP78 under optimal condition. International Journal of Environmental Science and Technology 7 (3), 457-464.
Gianfreda L, Sannino F, Rao MA, Bollag JM. 2003. Oxidative transformation of phenols in aqueous mixtures. Water Research 37, 3205-3215.
GonCalves I, Gomes A, Bras R, Ferra MIA, Amorim MTP, Porter RS. 2000. Biological treatment of effluent containing textile dyes. Journal of the Society of Dyers and Colourists 6, 393-397.
Kariminiaae-Hamedaani HR, Sakurai A, Sakakibara M. 2007. Decolourization of synthetic dyes by a new manganese peroxidase producing white rot fungus, Dyes and Pigments 72,157–162.
Kiseleva MG, Pimenova VV, Eller KI. 2002. Optimization of Conditions for the HPLC Determination of Synthetic Dyes in Food. Journal of Analytical Chemistry 58 (7), 685-690.
Liu W, Chao Y, Yang X, Bao H, Qian S. 2004. Biodecolourization of azo, anthraquinonic and triphenylmethane dyes by white-rot fungi and a laccase secreting engineered strain. Journal of Industrial and Microbiology and Biotechnology 31, 127-132.
Maciel M, Castro de Silva A, Ribeiro H. 2010. Industrial and biotechnological applications of ligninolytic enzymes of the basidiomycota: A Review Electronic Journal of Biotechnology vol13-issue6-fulltext-2.
Mane VJ, Patil SS, Syed AA, Baig MMV. 2007. Bioconversion of low quality lignocellulosic agricultural wastes into edible protein Pleurotus sajor-caju (Fr.) singer. Journal of Zhejiang University B. Science 8(10), 745-751.
Marina ZB, Durda V. 2010 .White-rot fungi in phenols, dyes and other xenobiotics treatment – a brief review Croat. Journal of Food Science and Technology 2 (2), 34-47.
Maz Al M, Miah A, Rahman MH, Rahman MM, Yahia ASM. 2012. Effect of Varieties and Media on Mycelial Growth and Substrate on Spawn Production of Oyster Mushroom. Bangladesh Journal of Research 7(4), 361-366.
Mehta KB, Bhandal MS. 1988. Mycelial growth variation of six Pleurotus species at different temperatures. Indian Mushroom Journal 14, 64-65.
Mshandete AM, Cuff J. 2008. Cultivation of three types of indigenous wild edible mushrooms: Coprinus cinereus, Pleurotus flabellatus and Volvariella volvocea on composted sisal decortications residue in Tanzania. African Journal of Biotechnology 7, 4551.
Nadeem A, Baig S, Sheikh N. 2014. Mycotechnological production of laccase by Pleurotus ostreatus p1 And its inhibition study. The Journal of Animal & Plant Sciences 24 (2), 492-502.
Novotny C, Dias N, Kapanen A, Malachova K, Vandrovcova M, Itavaara M, Lima N. 2006. Comparative use of bacterial, algal and protozoan tests to study toxicity of azo- and anthraquinone dyes. Chemosphere 63, 1436–1442.
Papinutti VL, Forchiassin F. 2007. Lignocellulolytic enzymes from Fomes fomentarius growing in solid-state fermentation. Journal of Food Engineering 81, 54–59.
Parani K, Eyini M. 2012. Production of Ligninolytic Enzymes during Solid State Fermentation of Coffee Pulp by Selected Fungi. Science Research Reporter 2249-2321.
Rehana A, Muhammad T, Tahir R. 2007. Propagation of Pleurotus sajor caju (Oyster Mushroom) Through Tissue Culture. Pakistan Journal of Botany 39(4), 1383-1386.
Roushdy MM, Abdel-Shakour EH. 2011. Potential Biotechnological Application of Lignin Peroxidase Produced by Cunninghamella elegans in the Decolourization and Detoxification of Malachite Green Dye. New York Science Journal 4(8), 80-88.
Sadia A, Muhammad A. 2011. Partial Purification and Characterization of Ligninolytic Enzymes Produced By Pleurotus ostreatus during Solid State Fermentation. African Journal of Biotechnology 10 (77), 17875-17883.
Selvam K, Swaminathan K, Chae KS. 2003. Decolourization of azo dyes and a dye industry effluent by white rot fungus. Bioresource Technology. 88, 115-119.
Shittu OB, Alofe FV, Onawunmi GO, Ogundaini AO, Tiwalade TA. 2005. Mycelial Growth and Antibacterial Metabolite Production by Wild Mushrooms. African Journal of Biomedical Research 8, 157 – 162.
Singh DA, Sabaratnam V, Abdullah N, Annuar MSM, Ramachandran KB. 2010. Decolourisation of chemically different dyes by enzymes from spent compost of Pleurotus sajor-caju and their kinetics. African Journal of Biotechnology Vol. 9 (1), 41-54.
Tien M, Kirk TK. 1988. Lignin peroxidase of Phanerochaete chrysosporium, Methods in Enzymology 161, 238–248.
Vikineswary S, Noorlidah A, Renuvathani M, Pandey MA, Jones EBG. 2006. Productivity of laccase in solid substrate fermentation of selected agroresidues by Pycnopruss anguineus. Bioresource Technology 97, 171-177.
Vishwakarma SK, Singh MP, Srivastava AK, Pandey VK. 2012. Azo Dye (Direct Blue 14) Decolourization by Immobilized Extracellular Enzymes of Pleurotus Species. Cellular and Molecular Biology 58 (1), 21-25.
Widiastuti H, Suharyanto AW, Sutamihardja H. 2008. Activity of Ligninolytic Enzymes during Growth and Fruiting Body Development of White Rot Fungi Omphalina sp and Pleurotus ostreatus. Hayati Journal of Biosciences Vol.15, No. 1978-3019.
Zeinab H, Said MBE, Saad MM, Douaa HAA. 2013. Optimization of cultural conditions for lignin peroxidase production by Phanerochaete chrysosporium and Pleurotus ostreatus. Academic Journal of Biotechnology 1(6), 87-95.
Patient D. Dhliwayo-Chiunzi, Ruvimbo Rwafa, Lydia Mugayi, Fungai Siwanja, Talent Ngurube, Glasnost Hudson, Hope Mvundura, Shumirai Nyashonjeka, Tapiwaishe Madeya , 2015. Screening and evaluation of ligninolytic dye decolourisation capacity of Pleurotus ostreatus. J. Biodiv. Environ. Sci., 6(3), 165-173.
Copyright © 2015 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.