Screening for antibacterial activity of some essential oils and evaluation of their synergistic effect

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Research Paper 01/04/2018
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Screening for antibacterial activity of some essential oils and evaluation of their synergistic effect

Heleili Nouzha, Belkadi Souhila, Merradi Manel, Oucheriah Yasmine, Ayachi Lamraoui R.
Int. J. Biosci.12( 4), 291-300, April 2018.
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Biological properties associated to many medicinal and aromatic plants have recently gained a great scientific interest. In this study, seven essential oils of famous plants reputed with their therapeutic vertus were screened in order to underline their limit antibacterial spectrum separately then establishing the correlated effect in their combination (antagonism, synergy). The antibacterial potential of seven essential oils was screened against ten Gram negative bacteria; E. coli (ATCCC 25922), Ps. aeruginosa (ATCC 27853), Acinetobacter sp, Klebsiella pneumonaie ESBL, Klebsiella oxytoca, Enterobacter sp, E. coli ESBL, Proteus sp, Morganella morganii, Pseudomonas aeruginosa MBL, Serratia sp and three strains of Gram-positive bacteria; S. aureus (ATCC 25923), Streptococcus sp, Staphylococcus aureus MRSA using disk diffusion method on agar medium (Mueller-Hinton agar), the strains were inoculated by swabbing technique and a volume of 25µl of each oil was tested. Synergy and antagonist effects were studied to evaluate single and binary combined antibacterial activities against reference strains (S. aureus, E.coli and Ps. aeruginosa).Results revealed a varing antibacterial activities against the examined pathogens according to the oil where ginger oil presented the highest activity and its larger inhibition zone (28.43 mm) toward the multidrug-resistant pathogen E. coli ESBL, while juniper, lavender and tarragon oils were the less effective ones.  Maximum activity of the tested essential oils was obtained from the combination of Ginger and rosemary essential oils against Staphylococcus aureus (36,36 mm) and E coli (34, 53 mm).In conclusion, the tested essential oils exhibited a very intense antibacterial potential toward the Gram-positive and the Gram negative bacteria even the drug-resistant ones.


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