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Seahorses and pipefishes: looking into its antimicrobial potential as used in traditional chinese medicine (TCM)

Sharon Rose Tabugo, Earth Khent Callo, Grazzette Anne Dablo, Charity Mae Apale

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Int. J. Biosci.15(1), 384-396, July 2019

DOI: http://dx.doi.org/10.12692/ijb/15.1.384-396


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Seahorses and pipefishes are important candidate organisms which have been used in Traditional Chinese Medicine (TCM). This study takes precedence in testing the antimicrobial potential of yellow seahorse (Hippocampus kuda) and scribbled pipefish (Corythoichthys intestinalis) crude extracts and decoction preparations with the following concentrations: 10%, 25%, 30%, 70% w/v. Kirby-Bauer disk diffusion test was used for the following test organisms: Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis. Observations from 24 to 72 hrs. were recorded. Photographs were taken for all the plates examined, processed and analyzed using UTHSCA Image tool software for measuring zones of inhibitions. Male and female pipefish crude extract preparations have effect on the test strains however, it yielded negative results on the decoction preparation. Inhibition zones were produced in all three (3) bacterial strains for the female pipefish while the male pipefish only produced inhibition zones against two (2) bacterial strains (P. aeruginosa and S. aureus). Noteworthy, various crude extract concentrations of male seahorse on B. subtilis and E. coli strikingly produced zones of inhibition. Varied concentrations of decoction preparation against B. subtilis and S. aureus also exhibit positive zones of inhibition. Noticeable zones of inhibition were commonly produced at 30 to 70% concentration for crude extracts. Results may support therapeutic claims albeit not comparable to commercial antibiotics. Moreover, it was observed that pipefishes classified as Least concern (LC) by IUCN produced more zones of inhibition thus, may alleviate pressure on rarer seahorses


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Seahorses and pipefishes: looking into its antimicrobial potential as used in traditional chinese medicine (TCM)

Alves RR, Rosa IL, Santana GG. 2007.The role of animal-derived remedies as complementary medicine in Brazil. AIBS Bulletin57, 949-955. https://doi.org/10.1641/B571107

Bauer AW, Kirby WM, Sherris JC, Turck M. 1966. Antibiotic susceptibility testing by a standardized single disk method. American journal of clinical pathology 45,493. https://doi.org/10.1093/ajcp/45.4_ts.493

Celino FT, HilomenGarcia GV, del NorteCampos AG. 2012. Feeding selectivity of the seahorse, Hippocampus kuda (Bleeker), juveniles under laboratory conditions. Aquaculture Research 43, 1804-1815. https://doi.org/10.1111/j.1365-2109.2011.02988.x

Cheesbrough M. 1984. Medical laboratory manualfor tropical countries vol11-microbiology.

Cole  AM, Weis P, Diamond G. 1997. Isolation and characterization of pleurocidin, an antimicrobial peptide in the skin secretions of winter flounder. Journal of Biological Chemistry 272, 12008-12013. http://dx.doi.org/10.1074/jbc.272.18.12008

Dawson CE. 1985. Indo-Pacific pipefishes (Red Sea to the Americas). The Gulf of Coast Research Laboratory Ocean Springs, Mississipi, USA.

Duda Jr TF, Kohn AJ, Palumbi SR. 2001.Origins of diverse feeding ecologies within Conus, a genus of venomous marine gastropods. Biological Journal of the Linnean Society 73,391-409. https://doi.org/10.1111/j.1095-8312.2001.tb01369.x

Ellis AE. 2001. Innate host defense mechanisms of fish against viruses and bacteria. Developmental & Comparative Immunology 25, 827-839. https://doi.org/10.1016/S0145-305X(01)00038-6

Hudzicki J. 2009. Kirby-Bauer Disk Diffusion Susceptibility Test Protocol.

Khora SS. 2013. Marine fish-derived bioactive peptides and proteins for human therapeutics. International Journal of Pharmacy and Pharmaceutical Sciences 5, 31-37.

Kohn AJ. 1968. Microhabitats, abundance and food of Conus on atoll reefs in the Maldive and Chagos Islands. Ecology 49, 1046-1062. https://doi.org/10.2307/1934489

Kohn AJ. 1959. The ecology of Conus in Hawaii. Ecological Monographs 29, 47-90. https://doi.org/10.2307/1948541

Kumaravel K, Ravichandran S, Balasubramanian T, Siva Subramanian K, Bilal AB. 2010. Antimicrobial effect of five seahorse species from Indian coast. British Journal of Pharmacology and Toxicology 1, 62-66.

Lourie SA, Foster SJ, Cooper EW, Vincent AC. 2004. A guide to the identification of seahorses. Project Seahorse and TRAFFIC North America, 114.

Magnadóttir B. 2006. Innate immunity of fish (overview). Fish & shellfish. Immunology 20, 137-151. https://doi.org/10.1016/j.fsi.2004.09.006

Plouffe DA, Hanington PC, Walsh JG, Wilson EC, Belosevic M. 2005.Comparison of select innate immune mechanisms of fish and mammals. Xenotransplantation 12, 266-277. https://doi.org/10.1111/j.1399-3089.2005.00227.x

Priya ER, Ravichandran S, Ezhilmathi R. 2013. Bioactive proteins from pipefishes. Journal of Coastal Life Medicine 1, 1-5.

Ravichandran S, Wahidulla S, D’Souza L, Rameshkumar G. 2010.Antimicrobial lipids from the hemolymph of brachyuran crabs. Applied biochemistry and biotechnology 162, 1039-1051. https://doi.org/10.1007/s12010-009-8.843-1

Remigio EA, Duda Jr TF. 2008.Evolution of ecological specialization and venom of a predatory marine gastropod. Molecular ecology 17, 1156-1162. https://doi.org/10.1111/j.1365-294X.2007.03.627.x

Sabuda DM, Laupland K, Pitout J, Dalton B, Rabin H, Louie T, Conly J. 2008. Utilization of colistin for treatment of multidrug-resistant Pseudomonas aeruginosa. Canadian Journal of Infectious Diseases and Medical Microbiology 19, 413-418. http://dx.doi.org/10.1155/2008/743197

Sanaye SV, Pise NM, Pawar AP, Parab PP, Sreepada RA, Pawar HB, Revankar AD. 2014. Evaluation of antioxidant activities in captive-bred cultured yellow seahorse. Aquaculture 434, 100-107. https://doi.org/10.1016/j.aquaculture.2014.08.007

Shapawi R, Tinin A, Eng HS. 2013. Total polyphenol content, anti-oxidative and anti-bacterial properties of seahorses traded as traditional medicine. International Journal of Natural Products Research 3, 68-73.

Vincent ACJ. 1996. The international trade in seahorses. Oxford: TRAFFIC International. Oxford University Press.


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