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Segregation disortion of anthocyanin morphological marker in F2 population of cross between basmati and environment genic male sterile rice lines

Research Paper | December 1, 2013

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Njiruh Paul Nthakanio, Kanya James Ireri, Kimani John Munji,Wanjogu Rapheal Kinyanjui, Kariuki Simon Njau

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Int. J. Agron. Agri. Res.3( 12), 43-52, December 2013


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Basmati rice (Oryza sativa L.) is preferred by many Kenyans yet its yield per hectare is low. This challenge led to initiation of Basmati hybrid rice project in Kenya with a goal of leaping yield benefits of heterosis. Since quality traits in Basmati are under recessive gene control, then the two parents in hybrid programme must possess the traits to avoid their masking at F1 by any other dominant gene. In the process of breeding for suitable parents, Basmati and Environmental genic male sterile lines V1-IR-73827-23-76-15-7S (V1), V2-IR-77271-42-5-4-36S (V2) V3-IR-75589-31-27-8-33S (V3) were crossed to produce F1. All the F1s plants had strong presence of anthocyanin on the base of their stems but segregated at F2 population. Thus, the objective of this study work was to evaluate and understand the mode of anthocyanin gene segregation at F2 population as a potential morphological marker. The F2 population was analyzed to test hypothesis that anthocyanin marker(s) is under simple Mendelian gene control. However, the results obtained indicated that F2 did not obey the Mendelian laws of gene segregation. This led to the conclusion that anthocyanin marker experience segregation distortion in F2 population.


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Segregation disortion of anthocyanin morphological marker in F2 population of cross between basmati and environment genic male sterile rice lines

Ahn SN, Bollich CN, Tanksley SD.1992. RFLP tagging of a gene for aroma in rice. Theoretic and Applied Genetics. 84, 825–828.

AFIDEP. 2012. www.populationaction.org Population, Climate Change, and Sustainable Development in Kenya. African Institute for Development policy, Population Action International and the African Institute for Development Policy, Nairobi, Kenya.

Ali J, Siddiq EA, Zaman FU, Abraham MJ, Ahmed I. 1995. Identification and characterization of Temperature sensitive genic male sterile sources in rice (Oryza sativa .L). Indian Journal of Genetics 55 (3), 243-259.

Bagali PG, Prabhu AHPD, Raghavendra K, Bagali PG, Hittalmani S, Vadivelu JS. 2010. Application of Molecular Markers in Plant Tissue Culture. Molecular Biology and Biotechnology 18 (1), 85-87.

Bert CYC, Casiana M, Vera C, Kenneth L, McNally, Parminder SV, Mackill DJ. 2008. Rice Molecular Breeding Laboratories in the Genomics Era: Current Status and Future Considerations. International Journal of Plant Genomics 208, 1-25.

Bradshaw HD (jr) Stettler RF. 1994. Molecular growth of genetics and development of Papulus, II, segregation due to genetic load. Theoretical and Applied Genetics. 89, 552-558

Dalmacio RD, Brar DS, Ishii T, Sitch LA, Virmani SS, Khush GS. 1995. Identification and transfer of a newcytoplasmic male sterility source form Oryza perennis into indica (Oryza sativa L.). Euphytica 82, 221-225.

De Jong W.S, Eannetta N.T, De Jong D.M, Bodis M .2004. Candidate gene analysis of anthocyanin pigmentation loci in the Solanacea. Theoretic and Applied Genetics.. 108, 423–432.

Edwards MD, Stuber CW, Wendel JF .1987. Molecular-marker-facilitated investigations of quantitative-trait loci in maize. I. Numbers, genomic Ik distribution and types of gene action. Genetics 116,113–125.

FAO .2006. Prospects for food, nutrition, agriculture and major commodity groups. World agriculture: towards 2030/2050. Global Perspective Studies Unit Food and Agriculture Organization of the United Nations Rome, Italy.

FAO. 2008. Climate Change, Water and Food Security, FAO Water Reports 36. Food and Agriculture Organization of the United Nations, Viale delle Terme di Caracalla, 00100 Rome, Italy.

GoK (Government of Kenya) .2007. Kenya Vision 2030. Ministry of Planning and National Development, Treasury Building, P. O. Box 30005,00200, Nairobi, Kenya.

Graner A, Jahoor A, Schondelmaier J, Siedler H, Pillen K, Fischbeck G, Wenzel G,Herrmann RG (1991) Construction of an RFLP map of barley. Theoretic and Applied Genetics. 83, 250–256.

IRRI (International Rice Research Institute) .2010. Accelerating Hybrid rice development. Edited by Xie F. and Hardy B. IRRI. Metro Manila, DAPO Box 7777, Philippines 2009, p 3-8.

Guiderdoni E. 1989. Comparative gametic selection in sexual and anther culture derivatives from five distant F1 hybrids of rice. Rice Genetics Newsletter 6, 91-92.

Harushima Y, Kurata N, Nagamura Y, Sasaki T, Minobe Y, Nakagahra M.1996. Detection of segregation distortion in an indicajaponica rice cross using a high-resolution molecular map. Theoretic and Applied Genetics. 92, 145-150.

Holton TA, Cornish EC. 1995. Genetics and Biochemistry of Anthocyanin Biosynthesis. The Plant Cell Vol.7,1071-1083.

Kanya JI, Njiruh PN, Kimani John M, Wajogu RKi, Kariuki SN . 2013. Evaluation of photoperiod and thermosensitive genic male sterile lines for hybrid rice seeds production in Kenya. International Journal of Agronomy and Agricultural Research (IJAAR) Vol. 3, No. (2), p. 21-39.

Khush GS. 1994. Increasing the genetic yield potential of rice: prospects and approaches. International Rice commission Newsletter 43, 1-7.

Kortstee AJ, Khan SA, Helderman C, Trindade LM, Wu Y. Visser RGF, Brendolise C, Allan A, Schouten HJ, Jacobsen E .2011. Anthocyanin production as a potential visual selection marker during plant transformation. Transgenic Reserves 20, 253–1264.

Kreuzaler F, Ragg H, Fautz E, Kuhn, DN, Hahlbrock K. 1983. UV-induction of chalcone synthase mRNA in cell suspen- Sion cultures of fetmselinum hortense. Proceedings. of the National Academy of Sciences. USA. 80, 2591-2593.

Kropff M, Cassman K, Peng S, Matthews R, Setter T. 1994. Quantitative understanding of yield potential. In Workshop on Rice Yield Potential in Favorable Environments. Edited by Cassman K. International Rice Research Institute, Los Ban˜os, Philippines 1994, 21-38.

Ku SJ, Cho KH, Cho YJ, Baek WK, Kim S, Suh HS, Chung YY. 2001. Cytological Observation of Two Environmental Genic Male-Sterile Lines of Rice. Molecular Cell 12 (3), 403-406.

Landry BS, Kesseli RV, Ferrara B, Michelmore RW .1987. A genetic map of lettuce (Lactuca sativa L.) with restriction fragment length polymorphism, isozyme, disease resistance, and morphological markers. Genetics 116,331–337.

Lin SY, Ikehashi H, Yanagihara S, Kawashima K. 1992. Segregation distortion via male gametes in hybrids between Indica and Japonica or wide compatibility varieties of rice (Oryza sativa L.). Theoretic and Applied Genetics 84, 812-818.

Lopez MT, Virmani SS. 2000. Development of TGMS line for developing two-line rice hybrids for the tropics. Euphytica 114, 211-215.

McCouch SR, Kochert G, Yu ZH, Wang ZY, Khush GS, Coffman WR, Tanksley SD. 1988. Molecular mapping of rice chromosomes. Theoretical and Applied Genetics 76, 815-829

Ministry of Agriculture. 2010. The ministry of agriculture at a glance. Office of the Permanent secretary, Kenya.

Nakagahra M. 1972. Genetic mechanism on the distorted segregation of marker genes belonging to eleventh linkage group in cultivated rice. Japanese Journal of Breeding 22, 232–238.

Oka HI .1974. Analysis of genes controlling F1 sterility in rice by the use of isogenic lines. Genetices 77, 521-534.

Reddy OUK, Siddiq EA, Sarma NP, Ali J, Hussein AJ, Nimmakayala P, Ramasamy P, Pammi S, Reddy AS .2000. Genetic Analysis of temperature-sensitive male sterility in rice. Theoretic and Applied Genetics 1000, 794-801.

Roger D. 2000. The importance of red pigment to plant life: experiment with anthocyanin. Journal of Biological Education 34(4), 2006-2010.

Sano Y. 1990. The genetic nature of gamete eliminator in rice. Genetics 125, 183–191.

Shanti ML, Shenoy VV, Devi GL, Kumar VM, Premalatha P, Kumar GN, Shashidhar HE, Zehr UB, Freeman WH. 2010. Marker-Assisted Breeding for Resistance to Bacterial Leaf Blight in popular cultivar and parental lines of hybrid rice. Journal of Plant Pathology 92 (2), 495-501.

Shi MS. 1981. Preliminary research report on breeding and utilization of the natural two-uses line in late japonica rice. Scientia Agriculturae Hubei 7, 1-3.

Shi MS. 1985. The discovery and study of the photosensitive recessive male-sterile (Oryza sativa L. subsp. japonica). Scientia Agriculturae Sinica 19, 44-48.

Shi MS, Deng JY. 1986. The Discovery, Determination and Utilization of the Hubei Photosensitive Genic Male-sterile Rice (Oryza sativa subsp. japonica). Acta Genetica Sinica 13(2), 107-112.

Virmani SS. 1994. Heterosis and Hybrid Rice Breeding. Monographs on Theoretical and Applied Genetics 22, p 7.1 Springer-Verlag. Berlin Heidelberg, Germany.

Virmani SS. 1996. “Hybrid Rice”, Advances in Agronomy.Vol. 57. Academic Press Inc. p 377-462.

Virmani SS, Sharma, HL. 1992. Manual for Hybrid seed production. International Rice Research Institute. DAPO Box 7777, Metro Manila, Philippines.

Virmani SS, Sun ZX, Mao TM, Jauhar Ali A, Mao CX. 2003. Two-Line Hybrid Rice Breeding Manual. p 4-85. IRRI. Manila, Philippines.

Wang B, Xu WW, Wang, JZ, Zheng HG, Yang ZY, Ray JD, Nguyen HT. 1995. “Tagging and mapping the thermosensitive genetic male sterile gene in rice (Oryza sativa L.) with molecular markers”. Theoretical Applied Genetics 91, 1111-1114.

Xie F, Hardy B. 2009. Accelerating Hybrid rice development. p 3-8. IRRI. Manila, Philippines.

Xu Y, Zhu L, Xiao J, Huang N, McCOuch S. R .1987. Chromosome regions associated with segregation distortion of molecular marker in F2, Backcross, doubled haploid and recombinant inbred lines in rice. Molecular General Genetics 253, 535-545.

Xu YB, Crouch JH. 2008. Review and Interpretation, Marker-Assisted Selection in Plant Breeding: From Publications to Practice. Crop Science –USA. 48: 391-407.

Yamagishi M, Takeuchi Y, Tanaka I, Kono I, Murai k, Yano M. 1210. Segregation distortion in f2 and doubled haploid populations of temperate japonica rice, Journal of Genetics. 89, 237–241.

Yang SR, Zhang LB, Chen WF, Xu ZJ, Wang JM. 1996. Theories and Methods of Rice Breeding for Maximum Yied. Acta Agronomica Sinica 22 (3), 295-304.

Yuan LP. 1994. Increasing yield potential in rice by exploitation of heterosis. In: Virmani SS, editor. Hybrid rice technology: new developments and future prospects. Selected papers from the International Rice Research Confernce. Manila (Philippines): International Rice Research Institute. p 1-6.

Zhang QF, Zhen BZ, Dai XK, Mei MH, SaghaMaroof MA, Li ZB.1994. Using bulked extremes and recessive class to map genes for Photoperiod-sensitive genic male sterility in rice. Proceedings of the National Academy of Sciences USA. 91, 8675- 86789.