Int. J. Biosci.15( 1), 361-374, July 2019
The biological approach is the most preferred technique for preparation of nanoparticles being much easy, less time consuming, and more importantly eco-friendly. In the current study, green synthesis of silver nanoparticles (AgNPs) was carried out by using Aloe vera gel. Characterization of the biosynthesized AgNPs was conducted by UV-vis spectrophotometer which showed the absorbance peak at the 450 nm. The Scanning Electron Microscopic (SEM) analysis revealed further, the spherical shaped AgNPs with the average size of 40nm±4. Nonetheless, the elemental composition and phase centered cubic crystalline nature was confirmed by the Energy Dispersive X-ray (EDX) and X-ray Powder Diffraction (XRD) respectively. The involvement of the carboxyl group of lipids of Aloe vera essential oils in the reduction of Ag cations to AgNPs was confirmed by the Fourier-transform infrared spectroscopy (FTIR). Moreover, the bio-synthesized AgNPs when subjected to evaluation of bactericidal activity against both the Gram-positive (Bacillus subtilis, Micrococcus luteus) and Gram-negative bacterial strains (Escherichia coli, Enterobacter aerogenes and Agrobacterium tumefaciens), showed higher activity against the Gram-negative bacterial strains. Furthermore, the particles under the study were also found to have potential to inhibit the growth of tested fungal strains (Mucor species, Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus and Fusarium solani) in a concentration-dependent manner. Cytotoxicity against HePG2 and MCF7 cells was also found significant with IC50 of 13.89 and 19.29 µM, respectively. Thus, it can be concluded that combination therapy of medicinal plants with metal nanoparticles might be one of the potential approaches to combat various diseases.
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