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Staphylococcus aureus in cow milk: Prevalence, antibiotic resistance and hygiene implications

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Research Paper 05/02/2024
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Staphylococcus aureus in cow milk: Prevalence, antibiotic resistance and hygiene implications

Syed Jawad Ali Shah, Adeena Nazir, Atif Bilal, Falak Niaz, Muhammad Asim, Muhammad Ilyas
Int. J. Biomol. & Biomed.18( 1), 20-25, February 2024.
Certificate: IJBB 2024 [Generate Certificate]
Key: Antibiotic resistanceDairy product safetyFarm hygienePenicillinS. aureus

Abstract

Staphylococcus aureus is a prominent human pathogen that is typically found in a variety of human anatomical positions. It is a Gram-positive, non-motile, and facultative anaerobic bacteria. A study was carried out to determine the prevalence of and antibiotic susceptibility pattern of  S. aureus isolated from milk. In Peshawar, 100 samples of cow milk were collected from various farms and processed. S. aureus was isolated and identified by inoculation onto Mannitol Salt Agar, gram staining and biochemical analysis. The Kirby Bauer disc diffusion method was used to assess the isolated S. aureus strains for antibiotic susceptibility. By measuring zone sizes and interpreting the data in accordance with predetermined standards.  S. aureus prevalence was identified and its patterns of antibiotic susceptibility were evaluated in samples of cow milk. S. aureus was found in 32 of the total 100 samples. Penicillin showed 100% full resistance, followed by Vancomycin (81.2%), Tetracycline (78.1%), and Erythromycin (75%), according to an antimicrobial susceptibility analysis that revealed substantial resistance trends. Contrarily, Gentamicin (84.3%), Trimethoprim-Sulfamethoxazole (75%), Chloramphenicol (59.3%), and Cefoxitin (56.2%) showed the lowest efficacy levels, whereas Ciprofloxacin (90%), which had the highest efficacy. In conclusion, S. aureus was detected in 32% of the 100 samples. Resistance to key antibiotics like Penicillin and Vancomycin was pronounced, highlighting the need for improved farm hygiene. Ciprofloxacin’s efficacy suggests its potential use, while overall, maintaining stringent sanitation practices is crucial for ensuring safe dairy products.

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