home icon user icon
Welcome to International Network for Natural Sciences | INNS Pub.
Home My Account
Submit Manuscript
double_slash
breadcumb_bg

Staphylococcus aureus in cow milk: Prevalence, antibiotic resistance and hygiene implications

Paper Details

Research Paper 05/02/2024
Views (440) Download (63)
current_issue_feature_image
publication_file

Staphylococcus aureus in cow milk: Prevalence, antibiotic resistance and hygiene implications

Syed Jawad Ali Shah, Adeena Nazir, Atif Bilal, Falak Niaz, Muhammad Asim, Muhammad Ilyas
Int. J. Biomol. & Biomed.18( 1), 20-25, February 2024.
Certificate: IJBB 2024 [Generate Certificate]
Key: Antibiotic resistanceDairy product safetyFarm hygienePenicillinS. aureus

Abstract

Staphylococcus aureus is a prominent human pathogen that is typically found in a variety of human anatomical positions. It is a Gram-positive, non-motile, and facultative anaerobic bacteria. A study was carried out to determine the prevalence of and antibiotic susceptibility pattern of  S. aureus isolated from milk. In Peshawar, 100 samples of cow milk were collected from various farms and processed. S. aureus was isolated and identified by inoculation onto Mannitol Salt Agar, gram staining and biochemical analysis. The Kirby Bauer disc diffusion method was used to assess the isolated S. aureus strains for antibiotic susceptibility. By measuring zone sizes and interpreting the data in accordance with predetermined standards.  S. aureus prevalence was identified and its patterns of antibiotic susceptibility were evaluated in samples of cow milk. S. aureus was found in 32 of the total 100 samples. Penicillin showed 100% full resistance, followed by Vancomycin (81.2%), Tetracycline (78.1%), and Erythromycin (75%), according to an antimicrobial susceptibility analysis that revealed substantial resistance trends. Contrarily, Gentamicin (84.3%), Trimethoprim-Sulfamethoxazole (75%), Chloramphenicol (59.3%), and Cefoxitin (56.2%) showed the lowest efficacy levels, whereas Ciprofloxacin (90%), which had the highest efficacy. In conclusion, S. aureus was detected in 32% of the 100 samples. Resistance to key antibiotics like Penicillin and Vancomycin was pronounced, highlighting the need for improved farm hygiene. Ciprofloxacin’s efficacy suggests its potential use, while overall, maintaining stringent sanitation practices is crucial for ensuring safe dairy products.

VIEWS 192
Cover PDF

Abd El Halem SGJAJOFS, Technology. 2019. Prevalence and antibiotic resistance of Staphylococcus aureus isolated from raw milk and dairy products collected from Alexandria, Egypt. 16(2), 25-33.

Agban MN, Ahmed ASJEJOMM.  2013. Detection and identification of Staphylococcus aureus enterotoxins in some milk products and their handlers. 22(2), 101-112.

Billa G, Thakkar K, Jaiswar S, Dhodi DJAHE, Policy H. 2014. A cross-sectional study to evaluate the awareness and attitudes of physicians towards reducing the cost of prescription drugs, Mumbai. 12, 125-137.

Cheung GY, Bae JS, Otto MJV. 2021. Pathogenicity and virulence of Staphylococcus aureus. 12(1), 547-569.

Foster TJJFMR. 2017. Antibiotic resistance in Staphylococcus aureus. Current status and Future Prospects. 41(3), 430-449.

Guo Y, Song G, Sun M, Wang J, Wang YJFIC, Microbiology I. 2020. Prevalence and therapies of antibiotic-resistance in Staphylococcus aureus. 10, 107.

Hiramatsu K, Kayayama Y, Matsuo M, Aiba Y, Saito M, Hishinuma T, Iwamoto AJJOGAR. 2014. Vancomycin-intermediate resistance in Staphylococcus aureus. 2(4), 213-224.

Jamali H, Paydar M, Radmehr B, Ismail S, Dadrasnia AJFC. 2015. Prevalence and antimicrobial resistance of Staphylococcus aureus isolated from raw milk and dairy products. 54, 383-388.

Lee AS, De Lencastre H, Garau J, Kluytmans J, Malhotra-Kumar S, Peschel A, Harbarth SJNRDP. 2018. Methicillin-resistant Staphylococcus aureus. 4(1), 1-23.

Macharia JK. 2018. Prevalence, risk factors and antimicrobial resistance of Escherichia coli pathotypes isolated from livestock and rats in slums areas of Nairobi. University of Nairobi,

Machria M. 2016. Prevalence, susceptibility patterns and risk factors associated with Staphylococcus aureus presence in marketed milk and milk products within Nairobi city country, Kenya.

Makinde TM, Ako-Nai KA, Shittu AJAJOMR. 2019. A study on the isolation and analyzation of Staphylococcus aureus obtained from nasal samples of female students (Undergraduates and Graduates) at the Obafemi Awolowo University Campus. 7(1), 24-27.

Mansour AS, Wagih GES, Morgan SD, Elhariri M, El-Shabrawy MA, Abuelnaga AS, Elgabry EJVW. 2017. Detection of Staphylococcus aureus enterotoxigenic strains in bovine raw milk by reversed passive latex agglutination and multiplex polymerase chain reaction, 10(8), 843.

Pollitt EJ, Szkuta PT, Burns N, Foster SJJPP. 2018. Staphylococcus aureus infection dynamics. 14(6), e1007112.

Rainard P, Foucras G, Fitzgerald JR, Watts J, Koop G, Middleton JJT, Diseases E. 2018. Knowledge gaps and research priorities in Staphylococcus aureus Mastitis Control. 65, 149-165.

Regasa S, Mengistu S, Abraha AJVMI. 2019. Milk safety assessment, isolation, and antimicrobial susceptibility profile of Staphylococcus aureus in selected dairy farms of Mukaturi and Sululta town, Oromia Region, Ethiopia. 2019.

Salem H, El-Attar L, Omran EJJOH IOPH. 2016. Microbiological assessment of some parameters of Kariesh cheese sold by supermarkets and street vendors in Alexandria, Egypt. 46(2), 77-85.

Umaru GA, Kabir J, Umoh VJ, Bello M, Kwaga JKJIJODR, Technology. 2013. Methicillin-resistant Staphylococcus aureus (MRSA) in fresh and fermented Milk in Zaria and Kaduna, Nigeria. 3(3), 67-75.

Usman R, Mustapha B, Mohammed FJFS, Management Q. 2016. Isolation and identification of methicillin resistant Staphylococcus aureus (MRSA) from traditionally fermented milk “nono” and yoghurt in Kaduna Metropolis, Nigeria. 2(2), 1-21.

Submit Manuscript