Study of combining ability and gene action of cooking quality traits in rice (Oryza sativa L.) using line × tester analysis

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Research Paper 01/03/2014
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Study of combining ability and gene action of cooking quality traits in rice (Oryza sativa L.) using line × tester analysis

Morteza Maleki, Mohammad H. Fotokian, Farrokh Darvish Kajouri, Mohammad Zaman Nouri, Kayvan Agahi
J. Bio. Env. Sci.4( 3), 220-226, March 2014.
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The relative proportion of additive and non-additive genetic variances in a population can be used as a tool to study inheritance of traits. This research was an attempt to estimate the combining ability and action of genes associated with the cooking quality of rice. Four lines (restorer) and three testers (male sterile line) were crossed to obtain twelve F1 hybrids in a line × tester fashion at Rice Research Institute of Amol, Iran in 2010. In the 2011growing season, parents and hybrids were laid out in a randomized complete block design with five replications. After harvesting, seeds were characterized for amylose content (AC), gelatinization temperature (GT) and gel consistency (GC). In the present study, the analysis of variance for general combining ability (GCA) and specific combining ability (SCA) were significant for the traits with the exception of SCA variance for GT. The GCA effect was larger than the SCA effect for GT while the AC and GC had larger SCA. These results along with calculation of predictability factor revealed that traits AC and GC were governed by genes with dominance action while additive gene action was more important in the genetic control of GT.


Asfaliza R, Rafii MY, Saleh G, Omar O, Puteh A. 2012. Combining ability and heritability of selected rice varieties for grain quality traits. Australian Journal of Crop Science 6, 1718-1723.

Cagampang GB, Perez CM, Juliano BO. 1973. A gel consistency test for the eating quality of rice. Journal of the Science of Food and Agriculture 24, 1589-1594.

Chen BT, Peng ZM, Xu YQ. 1992. Genetic analysis of ricegelatinization temperature. Journal of Huazhong Agricultural University. 11, 115-119.

Cuevas RP, Daygon VD, Corpuz H, Nora L, Reinke R, Waters D, Fitzgerald M. 2010. Melting the secrets of gelatinization temperature in rice. 28th International Rice Research Conference, Hanoi, Vietnam.

Faruq G, Hadjim KMO, Meisner CA. 2004. Inheritance of gelatinization temperature in rice. International journal of agriculture & biology 6, 810-812.

Gnanamalar RP, Vivekanandan P. 2013. Combining ability analysis of grain quality traits in rice (Oryza sativa L.). Asian Journal of Plant Science and Research 3, 145-149.

Juliano BO. 1971. A simplified assay for milled rice amylose. Cereal Science Today 16 334-338.

Juliano BO. 1985. Criteria and Test for Rice Grain Quality, in: Champagne ET, ed. Rice Chemistry and Technology, American Association of Cereal Chemists, St. Paul, MN, USA. 443-513 p.

Kempthorne O. 1957. An introduction to genetic statisticsed. John Wiley & Sons, Inc., New York.

Kiani S, Ranjbar GA, Kazemitabar SK, Jelodar NB, Nowrozi M, Bagheri N. 2008. Inheritance of gelatinization temperature and gel consistency in rice (Oryza sativa L.). Journal of Applied Sciences 8, 1503-1510.

Kumar I, Khush GS. 1987. Genetic analysis of different amylose levels in rice. Crop Science 27, 1167-1172.

Little RR, Hilder GB, Dawson EH. 1958. Differential effect of dilute alkali on 25 varieties of milled white rice. Cereal Chemistry 35, 111-126.

Liu Qm, Jiang Jh, Niu Fa, He Yj, Hong Dl. 2013. QTL Analysis for seven quality traits of RIL population in japonica rice based on three genetic statistical models. Rice Science 20.

McKenzie KS, Rutger JN. 1983. Genetic analysis of amylose content, alkali spreading score, and grain dimensions in rice. Crop science 23 306-313.

Patil VA, Vashi RD, Patil PP, Shinde DA. 2012. Line × tester analysis in rice (Oryza sativa L.). Plant Archives 12 463-469.

Pooni HS, Kumar I, Khush GS. 1993. Genetical control of amylose content in a diallel set of rice crosses. Heredity 71 603-613.

Raju CHD, Krishna L, Raju CHS, Vanisree S, Reddy PN, Reddy BB. 2012. Ggene action and combining ability for quantitative traits in rice (Oryza sativa .L). Research Journal of ANGRAU 40, 60-68.

Rastogi A, Mishra BK, Srivastava M, Siddiqui A, Shukla S. 2011. Biplot approach foridentification of heterotic crosses in linseed (Linum usitatissimum L.). Journal of Botany 11, 1-7.

Saidaiah P, Ramesha MS, Sudheer Kumar S, Suresh J. 2011. Combining ability analysis for yield and yield component traits in rice (Oryza sativa L.). Journal of Reserach ANGRAU 39, 28-33.

Singh NB, Singh HG, Singh P. 1977. Heterosis and combining ability for quality components in rice. Indian Journal of Genetics Plant Breeding 37, 347-352.

Singh SKHB, Sharma JK. 2007. Combining ability analysis for grain yield and other associated traits in rice. Oryza 44, 108-114.

Tan YF, Li JX, Yu SB, Xing YZ, Xu CG, Zhang Q. 1999. The three important traits for cooking and eating quality of rice grains are controlled by a single locus in an elite rice hybrid, Shanyou 63. Theoretical and Applied Genetics 99, 642-648.

Tang SX, KHUSH GS, Juliano BO. 1991. Genetics of gel consistency in rice (Oryza sativa L.). Journal of Genetics 70, 69-78.

Tiwari DK, Pandey P, Giri SP, Dwivedi JL. 2011. Nature of gene action and combining ability for yield and its related traits in hybrid rice. Oryza 48, 288-296.