home icon user icon
Welcome to International Network for Natural Sciences | INNS Pub.
Home My Account
Submit Manuscript
double_slash
breadcumb_bg

Study of lectin, antioxidant, cytotoxicity and anticancer properties of Punica granatum fruit juice against EAC cells in Swiss Albino mice

Paper Details

Research Paper 01/02/2019
Views (275) Download (9)
current_issue_feature_image
publication_file

Study of lectin, antioxidant, cytotoxicity and anticancer properties of Punica granatum fruit juice against EAC cells in Swiss Albino mice

Umme Habiba, Masum Miah, Ratna Khatun, Ajmeri Sultana, Mutasim Billah, Sadiya Tahsin, Farjana Binta Omar, Sumon Mohanto, Sumon Karmakar, Shafi Mahmud, Shafi Mahmub, Md. Abu Reza
Int. J. Biosci.14( 2), 481-488, February 2019.
Certificate: IJB 2019 [Generate Certificate]
Key: AnticancerAntioxidantCytoxicityEAC cellsLectinPunica granatum L

Abstract

Natural compounds from our daily diet can suppress or reverse the progression of cancer. Fruits and vegetables consumption are inversely associated with the reduction of cancer incidence and mortality. Antioxidants from fruits have been extensively studied for their free radical scavenging activities to prevent the occurrence of chronic degenerative diseases. Pomegranate (Punica granatum L.) fruit is known to have interesting pharmaceuticals activities and of great interest in biomedical research now a days. This fruit has been shown to exert anticancer and antioxidant activity which is generally attributed to its high content of polyphenols due to their effects of neutralizing free radicals. Current research was aimed to find out the presence or absence of anticancer activity of pomegranate fruit juice. The hemagglutination assay was performed in 96-well microtiter U-bottomed plates to determine the presence of lectin protein at the lower concentration of 52 µg/100 µL. When pomegranate juice was injected to EAC bearing mice for 5 days, it significantly decreased cancerous cells and percentage of cell growth inhibition was 56.28%. Apoptotic changes of EAC cells from treated mice were determined by light fluorescence microscopy. Interestingly, cell growth inhibition was clearly visible with pomegranate juice significantly. Further studies can be carried out to identify the lead compound responsible for anticancer activities of P. granatum fruit juice.

VIEWS 6
Cover PDF

Alabri THA, Al Musalami AHS, Hossain MA, Weli AM, Al-Riyami Q. 2014. Comparative study of phytochemical screening, antioxidant and antimicrobial capacities of fresh and dry leaves crude plant extracts of Datura metel L. Journal of King Saud University-Science 26(3), 237-243.

Bala A, Kar B, Haldar PK, Mazumder UK, Bera S. 2010. Evaluation of anticancer activity of Cleome gynandra on Ehrlich’s Ascites Carcinoma treated mice. Journal of Ethnopharmacology 129(1), 131-134.

Birben E, Sahiner UM, Sackesen C, Erzurum, S, Kalayci O. 2012. Oxidative stress and antioxidant defense. World Allergy Organization Journal 5(1), 9.

Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. 2018. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: a cancer journal for clinicians 68(6), 394-424.

Chaveerach A, Lertsatitthanakorn P, Tanee T, Puangjit N, Patarapadungkit N, Sudmoon R. 2016. Chemical constituents, antioxidant property, cytotoxicity and genotoxicity of Tiliacora triandra. Int. J. Pharmacogn. Phytochem. Research 8, 722-729.

da Silva PM, Baldry M, Peng P, de Oliveira Silva JN, Soares T, Brayner FA, Ingmer H. 2019. Punica granatum sarcotesta lectin (PgTeL) impairs growth, structure, viability, aggregation, and biofilm formation ability of Staphylococcus aureus clinical isolates. International journal of biological macromolecules 123, 600-608.

Hajleh MA, Al-Dujaili EA. 2016. Anti-Cancer Activity of Pomegranate and its Biophenols; General Review. EC Nutrition 6, 28-52.

Haldar PK, Kar B, Bala A, Bhattacharya  S, Mazumder UK. 2010. Antitumor activity of Sansevieria roxburghiana rhizome against Ehrlich ascites carcinoma in mice. Pharmaceutical Biology, 48(12), 1337-1343.

Huang WY, Cai YZ, Zhang Y. 2009. Natural phenolic compounds from medicinal herbs and dietary plants: potential use for cancer prevention. Nutrition and cancer 62(1), 1-20.

Kabir SR, Nabi MM, Haque A, Zaman RU, Mahmud ZH, Reza MA. 2013. Pea lectin inhibits growth of Ehrlich ascites carcinoma cells by inducing apoptosis and G2/M cell cycle arrest in vivo in mice. Phytomedicine 20(14), 1288-1296.

Kaltner H, Caballero GG, Ludwig AK, Manning JC, Gabius HJ. 2018. From glycophenotyping by (plant) lectin histochemistry to defining functionality of glycans by pairing with endogenous lectins. Histochemistry and cell biology, 1-22.

Kerr JF, Wyllie AH, Currie AR. 1972. Apoptosis: a basic biological phenomenon with wideranging implications in tissue kinetics. British journal of cancer 26(4), 239.

Khan N, Afaq F, Mukhtar H. 2008. Cancer chemoprevention through dietary antioxidants: progress and promise. Antioxidants & redox signaling 10(3), 475-510.

Meyer B, Ferrigni N, Putnam J, Jacobsen L, Nichols DJ, McLaughlin JL. 1982. Brine shrimp: a convenient general bioassay for active plant constituents. Planta medica 45(05), 31-34.

Nkondjock A, Robidoux A, Paredes Y, Narod S, Ghadirian P. 2006. Diet, lifestyle and BRCA-related breast cancer risk among French-Canadians. Breast cancer research and treatment, 98(3), 285-294.

Sharma P, McClees S, Afaq F. 2017. Pomegranate for prevention and treatment of cancer: an update. Molecules 22(1), 177.

Turrini E, Ferruzzi L, Fimognari C. 2015. Potential effects of pomegranate polyphenols in cancer prevention and therapy. Oxidative medicine and cellular longevity, 2015.

Submit Manuscript