Study of the antibiotic resistance of Pseudomonas aeruginosa isolated from pus and urine samples of patients hospitalized at the University Hospital of Mother and Child (CHU-ME) of N’Djamena (Chad)
Paper Details
Study of the antibiotic resistance of Pseudomonas aeruginosa isolated from pus and urine samples of patients hospitalized at the University Hospital of Mother and Child (CHU-ME) of N’Djamena (Chad)
Abstract
The strains of Pseudomonas isolated from nosocomial infections are, in most cases, resistant to antibiotics. The present work was carried out in order to determine the antibiotic resistance of Pseudomonas aeruginosa isolated from various pus and urine samples of patients hospitalized in the gyneco-obstetrics and pediatrics departments of the University Hospital of Mother and Child (CHU-ME) of N’Djamena in Chad.This is a prospective analytical study conducted at the laboratory of the University Hospital of the Mother and Child, which took place from February to September 2021. The samples were analyzed according to the standard of the Antibiogram Committee of the French Society of Microbiology. Of the 602 samples analyzed, 26 were positive for Pseudomonas aeroginosa, including 8 in pus and 18 in urine, for an overall prevalence of 4.32%. Of the 13 antibiotics tested, the strains showed 100% resistance to ampicillin, ceftriaxone, cefoxitin, erythromycin, vancomycin and cotrimoxazole. A 92% resistance to piperacillin and gentamicin and a 77% resistance to aztreonam. The Pseudomonas aeruginosa strains tested remain 100% sensitive to Ciprofloxacin, levofloxacin and oxacillin. This will require a readjustment of the protocol for the management of infections involving Pseudomonas aeruginosa.Also, the sanitation of the service and equipment is an essential factor in the prevention of nosocomial infections within the center.
Alaoui R, Benchekroun FL, Jazouli N. 1994. Les abcès cérébraux d’origine otogène: à propos de 35 cas. Revue de laryngologie, d’otologie et de rhinologie 115(3), 183-186.
Alkhudhairy, Al-Shammari MMM. 2020. Prevalence of metallo-β-lactamase–producing Pseudomonas aeruginosa isolated from diabetic foot infections in Iraq New Microbe and New Infect; 35, 100-661.
Amazian K, Rossello J, Castella A, Sekkat S, Terzaki S, Dhidah L. 2010. Prevalence of nosocomial infections in 27 hospitals in the Mediterranean region. Eastern Mediterranean Health Journal 16, 1070-1078.
Attika Rehman, Wayne M, Patrick1, Iain L. Lamont. 2019. Mechanisms of ciprofloxacin resistance in Pseudomonas aeruginosa: new approaches to an old problem. Journal of Medical Microbiology 68, 1–10.
Behzadi P, Baráth Z, Gajdács M. 2021. It’s Not Easy Being Green: A Narrative Review on the Microbiology, Virulence and Therapeutic Prospects of Multidrug-Resistant Pseudomonas aeruginosa. Antibiotics 10, 42. https://doi.org/10.3390/antibiotics 10010042.
Bessimbaye N, Djimadoum M, Issakou BV, Claude O, Nicolas B, Abdelsalam T, Choua O. 2021. Biochemical profile and resistance phenotype of bacteria isolated from the operating site departments of the National Reference University Hospital of N’Djamena World Journal of Advanced Research and Reviews 10(01), 381–396.
Comité de l’antibiogramme de la société française de microbiologie. 2020. CASFM / Société Française de Microbiologie V1.2.
Cottalorda, S Dahyot, J Lebeurre, A Soares, M Réveillon. 2017. Caractérisation phénotypique et moléculaire d’isolats urinaires de Pseudomonas aeruginosa. RICAI Paris, France. Hal-02269321.
Fortes Deguenonvo Traoré K, Dia Badiane NM, Ya R, Cissoko Y, Diouf A, Lathe NA, Diouf SA, Cissé VMP, Manga MN, Ndour CT, Soumaré M, SOW AI, Seydi M. 2015. Résultat d’une enquête d’incidence des cas des infections nosocomiales a bactéries multiresistantes dans un centre hospitalier de Dakar (Sénégal). Revue Malienne d’Infectiologie et de la Microbiologie 5, 8-25.
Frikh Maleb A, Nyaledome Ablavi I, Elouennass M, Lemouer A. 2017. Pseudomonas aeruginosa: épidémiologie et état actuel des résistances étude rétrospective sur trois ans. Journal of Marine Science and Engineering 21(2).
Gill JS, Arora S, Khanna SP, Kumar KH. 2016. Prevalence of multidrug-resistant, extensively drug-resistant, and pandrug-resistant Pseudomonas aeruginosa from a tertiary level Intensive Care Unit. Journal of Global Infectious Diseases 8, 155-9.X.
Hanane Zahir, Ghizlane Draiss, Noureddine Rada, Aicha Abourrahouat, Imane Ait sab, Mohamed Sbihi, Mohammed Bouskraoui, Nabila Soraa. 2019. Écologie microbienne et sensibilité aux antibiotiques des bactéries isolées d’infections urinaires chez l’enfant au Maroc. Rev Franco des Labo. ; N° 511, 65-70.
Hassan MA, Cheikna Z, Ali HH, Yeri EH, Denis E, Doumani D, Roumane M, Abdelsalam T, Aly S. 2020. Survey of the Germ Antibiorésistance Implied In Infections of the Surgical Site Infection (SSI) To the General Hospital Of National Reference (HGNR) Of N’Djamena (CHAD)International Journal of Medical Science and Clinical Invention 7(11), 5086-5094. https://doi.org/10.18535/ijmsci/v7i11.05.
Kos V, Déraspe M, McLaughin RE, Whiteaker JD, Roy PH, Alm RA, Corbeil J, Gardner H. 2015. The Resistome of Pseudomonas aeruginosa in Relationship to Phenotypic Susceptibility. Antimicrob. Agents Chemother 59, 427–436.
López-Causapé C, Cabot G, del Barrio-Tofino E, Oliver A. 2018. The Versatile Mutational Resistome of Pseudomonas aeruginosa. Front. Microbiol 9, 685.
Ouchar Mahamat O, Lounnas M, Hide M, Dumont Y, Tidjani A, Kamougam K. 2019. High prevalence and characterization of extended-spectrum ß-lactamase producing Enterobacteriaceae in Chadian hospitals. BMC Infect Dis 28(1), 205.
Qayoom S, Rashid A, Kohli A, Masoodi T, Amin M. 2019. Prevalence and antibiotic sensitivity pattern of pseudomonas aeruginosa isolates from respiratory samples, pus samples and body fluids in a tertiary care hospital, Kashmir. Indian Journal Microbiology Research 6(4), 345-349.
Savadogo M, Dao L, Koueta F. 2015. Les infections à Pseudomonas aeruginosa au service des maladies infectieuses du CHU YO, Burkina Faso: à propos deux cas. Pan African Medical Journal 21, 78 https://doi.org/10.11604/pamj..21.78.4739.
Talebi-Taher, M, Majidpour A, Gholami A, Rasouli-Kouli S, Adabi M. 2016. Role of efflux pump inhibitor in decreasing antibiotic cross-resistance of Pseudomonas aeruginosa in a burn hospital in Iran. Journal of Infection Developing Countries 10, 600–604.
Yasmeen Islam S, Islam S, Uddin M, Jahan R. 2015. Prevalence of urinary tract infection, its causative agents and antibiotic sensitivity pattern: A study in Northern International Medical College Hospital, Dhaka. Northern International Medical College Journal 7(1).
Zohreh H, Eshagh B, Mohamad E, Ali Akbar J. 2016. Virulence genes profile of multidrug resistant Pseudomonas aeruginosa isolated from Iranian children with UTIs. Acta Med Iran 54(3), 201-210.
Ahmat Mahamat Ahmat, Hassan Mahamat Ali, Fissou Henry, Bertille Dewa, Ali Haroun Hissein, Kadidja Gamougam, Adoum Fouda Abderrazzack, Abdelsalam Tidjani, Choua Ouchemi (2022), Study of the antibiotic resistance of Pseudomonas aeruginosa isolated from pus and urine samples of patients hospitalized at the University Hospital of Mother and Child (CHU-ME) of N’Djamena (Chad); IJB, V21, N2, August, P271-278
https://innspub.net/study-of-the-antibiotic-resistance-of-pseudomonas-aeruginosa-isolated-from-pus-and-urine-samples-of-patients-hospitalized-at-the-university-hospital-of-mother-and-child-chu-me-of-ndjamena-chad/
Copyright © 2022
By Authors and International
Network for Natural Sciences
(INNSPUB) https://innspub.net
This article is published under the terms of the
Creative Commons Attribution License 4.0