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Study of the antibiotic resistance of Pseudomonas aeruginosa isolated from pus and urine samples of patients hospitalized at the University Hospital of Mother and Child (CHU-ME) of N’Djamena (Chad)

Research Paper | August 20, 2022

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Ahmat Mahamat Ahmat, Hassan Mahamat Ali, Fissou Henry, Bertille Dewa, Ali Haroun Hissein, Kadidja Gamougam, Adoum Fouda Abderrazzack, Abdelsalam Tidjani, Choua Ouchemi

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Int. J. Biosci.21( 2), 271-278, August 2022

DOI: http://dx.doi.org/10.12692/ijb/21.2.271-278


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The strains of Pseudomonas isolated from nosocomial infections are, in most cases, resistant to antibiotics. The present work was carried out in order to determine the antibiotic resistance of Pseudomonas aeruginosa isolated from various pus and urine samples of patients hospitalized in the gyneco-obstetrics and pediatrics departments of the University Hospital of Mother and Child (CHU-ME) of N’Djamena in Chad.This is a prospective analytical study conducted at the laboratory of the University Hospital of the Mother and Child, which took place from February to September 2021. The samples were analyzed according to the standard of the Antibiogram Committee of the French Society of Microbiology. Of the 602 samples analyzed, 26 were positive for Pseudomonas aeroginosa, including 8 in pus and 18 in urine, for an overall prevalence of 4.32%. Of the 13 antibiotics tested, the strains showed 100% resistance to ampicillin, ceftriaxone, cefoxitin, erythromycin, vancomycin and cotrimoxazole. A 92% resistance to piperacillin and gentamicin and a 77% resistance to aztreonam. The Pseudomonas aeruginosa strains tested remain 100% sensitive to Ciprofloxacin, levofloxacin and oxacillin. This will require a readjustment of the protocol for the management of infections involving Pseudomonas aeruginosa.Also, the sanitation of the service and equipment is an essential factor in the prevention of nosocomial infections within the center.


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Study of the antibiotic resistance of Pseudomonas aeruginosa isolated from pus and urine samples of patients hospitalized at the University Hospital of Mother and Child (CHU-ME) of N’Djamena (Chad)

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