Sugar and anthocyanin characterization of four Iranian pomegranate (Punica granatum L.) varieties using HPLC System

Paper Details

Research Paper 01/04/2014
Views (633)
current_issue_feature_image
publication_file

Sugar and anthocyanin characterization of four Iranian pomegranate (Punica granatum L.) varieties using HPLC System

Somayeh Mirzaee
J. Biodiv. & Environ. Sci. 4(4), 248-253, April 2014.
Copyright Statement: Copyright 2014; The Author(s).
License: CC BY-NC 4.0

Abstract

Among all types of fruit, pomegranate has been known as an excellent fruit due to its high valued nutraceutical components; i.e. anthocyanins, phenolic compounds and organic acids. This research aimed to measure anthocyanins and sugars in different varieties of pomegranate. Four cultivars were Vahshe Kane Tehran, Gorche Shahvar Yazdi, Shirin Shahvare Yazd and Pust sefid. High performance liquid chromatography (HPLC) was used in order to analyze the samples for measuring their components. Cyanidin 3-glucoside, cyanidin 3,5-diglucoside, delphinidin 3-glucoside, pelargonidin 3-glucoside and pelargonidin 3,5-diglucoside were detected in all samples as major anthocyanins. However, their quantities were different such that cyanidin 3,5-diglucoside was the main pigment, followed by cyanidin 3-glucoside and delphinidin 3-glucoside, while pelargonidin derivatives were always present in small amounts. Pust Sefid and Shirin Shahvare Yazd cultivars contained more anthocyanin compared to Gorche Shahvar Yazdi and Vahshe Kane Tehran. Results showed Fructose, glucose and sucrose were major sugars of the cultivars. Vahshe Kane Tehran had the highest fructose and sucrose content, while Gorche Shahvar Yazdi had the highest glucose content. Sugar content in Gorche Shahvar Yazdi and Vahshe Kane Tehran was more than that in Pust sefid and Shirin Shahvare Yazd cultivars.

Mousavinejad G, Emam-Djomeh Z, Rezaei K, Khodaparast MHH. 2009. Identification and quantification of phenolic compounds and their effects on antioxidant activity in pomegranate juices of eight Iranian cultivars”, Journal of Food Chemistry 115, 1274–1278. http://dx.doi.org/10.1016/j.foodchem.2009.01.044

Gil MI, Tomas-Barberan FA, Hess-Pierce B, Holcroft DM, Kader AA. 2000. Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing”, Journal of Agricultural and Food Chemistry 48, 4581–4589. 10.1021/jf000404a

Du C, Wang P, Francis FJ. 1975. Anthocyanins of pomegranate, Punica granatum. Journal of Food Science 40, 417–418.

Hulme AC. 1970. The biochemistry of fruits and their products”, Vol. 1. London and New York: Academic Press.

Mertens-Talcott SU, Jilma-Stohlawetz P, Rios J, Hingorani L, Derendorf H. 2006. Absorption, metabolism and antioxidant effects of pomegranate (Punica granatum L.) Polyphenols after ingestion of a standardized extract in healthy human volunteers”, Journal of Agricultural and Food Chemistry 54, 8956–8961.

Kong JM, Chia LS, Goh NK, Chia TF, Brouillard R. 2003. Analysis and biological activities of anthocyanins. Journal of Phytochemistry 64, 923–933. http://dx.doi.org/10.1016/S0031-9422(03)00438-2

Miguel  G,  Fontes  C,  Antunes  D,  Neves  A, Martins  D.  2004.  Anthocyanin  concentration  of “Assaria”  pomegranate  fruits  during  different  cold storage conditions”, Journal of Biomedicine and Biotechnology 5, 338-342. http://dx.doi.org/10.1155/S1110724304403076

Sepúlveda E, Sáenz C, Peña Á, Robert P, Bartolomé B, Gómez-Cordovés C. 2010. Influence of the genotype on the anthocyanin composition, antioxidant capacity and color of Chilean pomegranate (Punica granatum L.) juices”, Chilean Journal of Agricultural Research 70(1), 50-57.

Melgarejo P, Salazar DM, Artes F. 2000. Organic acids and Sugars composition of harvested pomegranate fruits”. Eur Food Res Technol 211, 185-190. 10.1007/s002170050021

Fadavi A, Barzegar M, Azizi MH, Bayat M. 2005. Physicochemical composition of ten pomegranate cultivars (Punica granatum L.) grown in Iran”, Food Science and Technology International 11, 113-119. 10.1177/1082013205052765

Related Articles

Dietary Aloe vera improves growth and hematology in Nile tilapia (Oreochromis niloticus)

Fatima Khan*, J. Biodiv. & Environ. Sci. 28(4), 89-99, April 2026.

Intercropping camphor basil shrubs with selected food crops for ecosystem services in the upper midland agroecological zone of Western Kenya

Reuben K. B. Chumba*, Alex Awiti, Francis Namasaka Muyekho, Vitalis Ogemah, Jacob Omollo, Yosef Kidane Gebrehawariat, J. Biodiv. & Environ. Sci. 28(4), 73-88, April 2026.

Surveillance and detection of African swine fever on abbatoir in different municipalities of third district of Cagayan, Philippines

Maricel F. Campanano, John Michael M. Melad, Mary Ann M. Santos*, J. Biodiv. & Environ. Sci. 28(4), 65-72, April 2026.

Mobile-based potato leaf disease identifier using ensemble modeling

Karen W. Cantilang*, Laarni M. Ladiao, J. Biodiv. & Environ. Sci. 28(4), 58-64, April 2026.

Diagnostic analysis of pig farms in the North of Côte d’Ivoire: Case of the commune of Korhogo

Seni Kouadio Sylvain*, Kadjo Vincent, Alla Konan Jean Bedel, Yao Koffi Sylvanus Aubert, N’glouan Wadjé Jérôme, Soro Ouation Souleymane, Kouassi Koffi Dongo, J. Biodiv. & Environ. Sci. 28(4), 48-57, April 2026.

Coral reef condition in Illana Bay, Zamboanga del Sur, Philippines: Basis for conservation management

Ruel S. Lasagas, Rosanilio M. Yagos*, Edgardo H. Rosales, J. Biodiv. & Environ. Sci. 28(4), 40-47, April 2026.

Preliminary floral and faunal species diversity in Maluyo River in Santol, La Union, Philippines

Judith M. Morales*, Analyn V. Sagun, Angelina T. Gonzales, J. Biodiv. & Environ. Sci. 28(4), 26-39, April 2026.

Challenges and impact of the farmer-scientists training program on community development in Bohol, Philippines

Jeffrey O. Awas*, Anabel J. Intong, Aida T. Salingay, Manolito C. Macalolot, J. Biodiv. & Environ. Sci. 28(4), 8-25, April 2026.