Synchronization of maturation in Silkworms, Bombyx mori L. (Lepidoptera: Bombycidae) using phytoecdysteroid from Purslane, Portulaca oleracea L.
Paper Details
Synchronization of maturation in Silkworms, Bombyx mori L. (Lepidoptera: Bombycidae) using phytoecdysteroid from Purslane, Portulaca oleracea L.
Abstract
Rearing of silkworms, Bombyx mori L. is an indispensable and significant activity of sericulture, the art and science of producing silk, the Queen of textiles. It is considered an arduous job and a critical concern is the unsynchronized maturation of silkworms on the onset of cocoon spinning requiring more time and manpower to complete mounting for cocoon production. This study determined the effect of phytoecdysteroid extracted from Portulaca oleracea L. on the synchronization of silkworm maturation to shorten the mounting time. High-performance liquid chromatography (HPLC) confirmed the presence of the phytoecdsyteroid, 20-hydroxyecdysone extracted from local plant, P. oleracea. The extract was sprayed with 50% and 100% concentrations (v/v) on mulberry leaves and administered to 5th instar silkworms different batches of silkworms at 116 hours, 92 hours, 76 hours, 52 hours and 28 hours before spinning. The time range and number of spinning silkworms during mounting process were recorded cumulatively. Results showed that both concentrations made approximately 50% synchronization of maturation in B. mori L. on the day of spinning at 6:30-7:59 AM and complete maturation at 8:00-9:59 AM. On the other hand, the negative control had approximately 50% synchronized maturation of silkworms between 10:00-11:59 AM and complete maturation at 1:00 PM. Phystoedysteroid from P. oleracea L. shortened the larval stage of silkworms and enhanced synchronization of maturation. Consequently, this study will help sericulture farmers consume lesser time and manpower of mounting matured silkworms.
Daniel M, Mammen D. 2014. Ecdysterone and Antioxidants in Purslane (Portulaca oleracea Linn.). Asian Journal of Chemical and Pharmaceutical Research, AJCPR 2(2), 137-139.
Kurian JC. 2010. Amazing Healing Plants. Volume 1. Philippine Publishing House, Manila, Philippines p.118
Ladion-De Guzman, Herminia. 1985. Healing Wonders of Herbs. Philippine Publishing House, Manila, Philippines p. 66.
Lafont R, Balducci C, Dinan L. 2021. Ecdysteroids. Encyclopedia 1, 1267-1302.
Naggar, Yahya Al, Mohamed Ghorab, Kariman Mohamed. 2017. Phytoecdysteroids: Isolation and Biological Applications. American Journal of Life Sciences 5(1), 7-10. DOI: 10.11648 /j.ajls.20170501.12.
Nair, Sashindran, Miao Yun-gen S, Nirmal Kumar. 2005. Differential Response of Silkworm, Bombyx mori L. to Phytoecdysteroid Depending on the Time of Administration. Journal of Applied Sciences and Environmental Management 9 (3), 81 -86.
Panndey P, Upadhyay VB. 2013. Impact of Phytoecdysteroid Treatment on the Larval Performance of Multivoltine Mulberry Silkworm Bombyx mori Linn. Malaysian Applied Biology Journal 42(1), 51-60.
Rufaie ZH. 2012. Effect of Phytoecdysteroid (β-Ecdysone) on Synchronization of Maturation in Silkworm Bombyx mori L. International Journal of Advanced Biological Research 2(2), 238-240.
Sangwan N. 2018. Plant Metabolic Engineering. Omics Technologies and Bioengineering. Towards Improving Quality of Life. Volume 2. Academic Press 143-175
Srivastava K, Upadhyay VB. 2012. Effect of the Phytoecdysteroids on length of Silk Filament and Non -Breakable Filament Length of Multivoltine Mulberry Silkworm Bombyx mori Linn. Academic Journal of Entomology 5(3), 174-181.
Srivastava K, Upadhyay VB. 2013. The Effect of Phytoecdysteroid on Silk Producing Potential of Multivoltine Mulberry Silkwotm Bombyx mori Linn. The Bioscan, An International Journal of Life Sciences 8(1), 43-47.
Uma A, Bhosale. 2011. Study of central nervous system depressant and behavioral activity of an ethanol extract of Achyranthes aspera (Agadha) in different animal models. Int J App Basic Med Res 1: 104-108. DOI: 10.4103/2229-516X.91154
Yan-Xi Zhou. 2015. “Portulaca oleracea L.: A Review of Phytochemistry and Pharmacological Effects.” Review Article. BioMed Research International. Vol 2015, Article ID 925631, Hindawi Publishing Corporation
Elizabeth P. Obra, Christian Geen E. Salazar (2022), Synchronization of maturation in Silkworms, Bombyx mori L. (Lepidoptera: Bombycidae) using phytoecdysteroid from Purslane, Portulaca oleracea L.; IJB, V21, N5, November, P192-199
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