The bactericidal effect of the Nd: YAG laser on the bacteria of the infected root canals: an in vitro study

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Research Paper 01/11/2018
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The bactericidal effect of the Nd: YAG laser on the bacteria of the infected root canals: an in vitro study

Hareth A. Alrikaby, Balsam S. Abdulhameed
Int. J. Biosci. 13(5), 483-490, November 2018.
Copyright Statement: Copyright 2018; The Author(s).
License: CC BY-NC 4.0

Abstract

It is very important to obtain the maximum sterilization and antibacterial effect during treatment of the infected root canals. This study aimed to explore the bactericidal effect of the Nd:YAG laser.  The method basically is based on irradiating a bacterial sample by Nd:YAG laser and calculating the number of colony forming units (that will appear on agar plates) before and after laser application. 30 swabs were taken from infected root canals immediately after tooth extraction and immersed in a tubes containing liquid nutritive media. These swabs undergone a serial dilution and then streaked on a blood agars and incubated. After 24 hours, the colony forming units were calculated. The sample (in the tube) then irradiated by laser, and also streaked on blood agar and incubated. The colony forming units were calculated again. Nd:YAG laser showed a weak bactericidal effect with about 25% of the bacterial cells were eradicated. based on the results of this study, the Nd:YAG laser was merely effective as a bactericidal agent.

Adam Stabholz, Sharonit Sahar-Helft, Joshua Moshonov. 2004. Lasers in endodontics. Dental clinics of North America web journal. https://doi.org/10.1016/j.cden.2004.05.012

Asnaashari M, Safavi N. 2013. Disinfection of Contaminated Canals by Different Laser Wavelengths while Performing Root Canal Therapy. Journal of  Lasers in Medical Science 4. (PMID:  25606308)

Berkiten, Mustafa, Rahmiye Berkiten, İmer Okar. 2000. “Comparative evaluation of antibacterial effects of Nd: YAG laser irradiation in root canals and dentinal tubules.” Journal of endodontics 26(5), 268-270. https://doi.org/10.1097/00004770-200005000-00003

Bettina Ruth Basrani. 2015. Endodontic irrigation: chemical disinfection of root canal system, pages 65-115. Springer.

Carlosde Paula, Eduardo Sheila, Gouw-Soares 2001. The Use of Lasers for Endodontic Applications in Dentistry, Medical Laser Application 16(3), 231-243 p. https://doi.org/10.1078/1615-1615-00027

Cohen, Stephen. 2006. Pathways of the Pulp 9th edition. Chapter one. Mosby.

Gunnar Bergenholtz. 2009. Textbook of Endodontology 2nd edition Chapter 7. Wiley Blackwell Inc.

Hossain M. 1999. Ablation depths and morphological changes in human enamel and dentin after Er: YAG laser irradiation with or without water mist. Journal of clinical laser medicine & surgery 17(3), 105-9. http://dx.doi.org/10.1089/clm.1999.17.105

Layla Mohammed Hassan Al–Aamirry. 2003. EFFECT OF He –Ne LASER (632.8) nm AND DIODE LASER (890) nm ON Streptococcus mutans ISOLATED FROM PATIENTS OF CARIOUS TEETH ( MSc thesis), laser institute for postgraduate studies, University of Baghdad.

Meire M. 2012. Evaluation of Nd:YAG and Er:YAG irradiation, antibacterial photodynamic therapy and sodium hypochlorite treatment on Enterococcus faecalis biofilms. International Endodontic Journal, 45. http://dx.doi.org/10.1111/j.1365-2591.2011.02000.x

Narayanan, Lakshmi L, Vaishnavi C. 2010. “Endodontic Microbiology.” Journal of Conservative Dentistry 13(4). http://dx.doi.org/10.4103/0972-0707.73386

Nisha Garg, Amish Garg. 2013. Textbook of endodontics 2ndedition. Pages 210-230. Jaypee Brothers Medical Publishers.

Philip D. Marsh, Michael V. Martin. 2009. Oral microbiology 5th edition, Chapter 7. Elsevier Inc.

Pirnat S, Matjaz Lukac, Alojz Ihan. 2010. Study of the direct bactericidal effect of Nd:YAG and diode laser parameters used in endodontics on pigmented and nonpigmented bacteria. Lasers in Medical Sciences 26. http://dx.doi.org/10.1007/s10103-010-0841-6

Rajendran R. 2009. Shafer’S Textbook Of Oral Pathology (6Th Edition). Section 2. Elsevier Inc.

Schoop U. 2004. Bactericidal effect of different laser systems in the deep layers of dentin. Lasers in Surgery and Medicine 35(2).  http://dx.doi.org/10.1002/lsm.20026

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