The effect of air pollution on leaves and pollen of Senecio bicolor in the region of Annaba, Algeria

Paper Details

Research Paper 01/10/2017
Views (371) Download (12)

The effect of air pollution on leaves and pollen of Senecio bicolor in the region of Annaba, Algeria

Nadia Tlili, Naziha Bourenane, Nadjette Aimeur, Larbi Boughediri
Int. J. Biosci.11( 4), 24-34, October 2017.
Certificate: IJB 2017 [Generate Certificate]


The Annaba city (North-East of Algeria) is characterized by a very polluted atmosphere due of the road traffic and two big industrial complexes, which have become a danger for the fauna and flora. The present work consists of using the leaves and pollen of Senecio bicolor of Asteraceae family as a bioindicators to estimate the degree of air pollution in the Annaba region. We measured the pH, MF/MS ratio, and assayed chlorophyll and proline, from leaves taken from four sites, which three are in the vicinity of the three air quality control network stations installed in Annaba. The fourth is a control site in Seraïdi. At the same sites we exposed to air, also, 1g of pollen for 24 hours. The analysis of air quality control network showed the existence of pollution caused especially by the dust notably at station 2 (80.14 µg/m3), far exceeds the WHO threshold (50 μg / m3). Our results showed that the amount of dust deposited on the leaf surfaces notably at station 2, causing significant disturbances in their metabolisms especially at the level of the chlorophyll content (40.22 – 20.52 µg/g), the content of the proline (24.38 – 98.89 µg/g) and viability of pollen (68.66 – 31.33 %) which can easily be observed compared to the control zone, which is far from the source of emission. In conclusion the bioindication by the leaves and the pollen seems to be effective to estimate the quality of the air.


Abdul W. 2004.Analysis of toxic and osmotic effects of sodium chloride on leaf growth and economic yield of sugarcane. Botanical bulletin of Academia Sinica45, 133-141.

AIRFOBEP. 1999 – 2000. Qualité de l’air de la région de l’Etang de Berreet de l’ouest des Bouches-du-rhône, Bilan de la période 1999 – 2000.

Ben Khaled L, Gomez AM, Ourraqi EM, Oihabi A. 2003.Physiological and biochemical responses to salt Stres of mycorrhized and/ornodulaled clover seedlings (Trifolium alexandrinum). Agronomie, 23, 571-580.

Braun S, Fluckiger W. 1985. Increased population of the aphid pomi at a motorway:Part 3- the effect of exhaust gases, Environmental pollution, 39 : 183- 192.

Cerceau-Larrival T, Derout L. 1988.Influence de la pollution environnementalesur la composition minérale des pollens allergisants de Dactylis glomerata (Poaceae). Conséquenceséventuelles en immuno-Allergie. Annales des sciences naturelles. Botanique, Tome 9, 163-180.

ChakhparoniaM. 1995. Development d’un systèmebiologique de surveillance de la pollution atmosphérique: étudessur des populations d’ Arabidopsis thaliana sous Conditions contrôlées, Thèse de doctorat, Université Lausanne,  81p.

Dagnelie P. 2009.  Statistique théorique et appliquée. Tome 2 : Inférence statistique à une et une à deux dimensions. Edition De BOECK ET LARCIER. Bruxelles, 659 p.

Garrec  C, Haluwyn V. 2002. Bio surveillance végétale de la qualité de l’air, Edition Tec et Doc. ISBN: 2-7430-0540-8. Lavoisier, 118 p.

Grodzinska K. 1982. Monitoring of air pollutants by mosses and tree bark. In: Steubing L., Jäger, H. J. (ed.). Monitoring of Air Pollutants by plants. Methods and Problems. Proceedings International Workshop. Dr. W. Junk Publishers, The Hague, Boston, London, 33-42.

Gupta AK, Mishra RM. 1994. Effect of lime kiln’s air pollution on some plant species, Pollution Research13(1), p. 1-9.

Hasnaoui N. 2000. Influence de la pollution atmosphérique globale sur certains caractères du pollen des arbres fruitiers de la famille des Rosacées dans la région d’Annaba, Mémoire de Magister, Universitéd’ Annaba (Algérie), 97p.

Holden M. 1975. Chlorophylls in chemistry and biochemistry of plant pigment. 2ndedition,TW. Goodwin. Edition Academic Press New York, 133.

Lagadie L, Caquet T, Amiard JC, Ramade F. 1997. Biomarqueurs en écotoxicologie. Aspects fondamentaux. Ed. Masson 196p.

Minitab Inc. 2016. Statistical software (release 16). Computer software. State college, PA: Minitab, INC

Monneveux D, Nemmar M. 1986. Contribution à l’étude de la résistance à la sécheresse chez le blé tendre (Triticum aestivum) et chez le blé dur (T. durum Desf) : Etude de l’accumulation de la proline au cours du cycle de développement Agronomie. 104, 57-62.

Panda SK. 2003. Heavy-metal phytotoxicity induces oxidative stress in a moss, Taxithellium sp. Science. 84(5), 10: 631-633.

Renaud JP, Laitat E, Mauffette Y, Allard G. 1998. Photoassimilate allocation and photosynthetic and biochemical characterisitics of two alfalfa (Medicago sativa) cultivars of different ozone sensitivities, Canadian journal of botany 76(2), 281-289 p.

Semadi A, De cormis L. 1986. Influence de la pollution atmosphérique fluorée sur la végétation de la région d’Annaba (Algérie), Pollution atmosphérique, 109, p.24-30.

Semadi A. 1989. Effets de la pollution atmosphérique sur la végétation dans la région d’Annaba, Thèse de Doctorat d’Etat en Science Naturelle, Universitéde Paris 6, 339p. 

Sharma GK. 1987. Plant cuticular compex in response to high way pollution. The science of the total Environement, Paris, 59, 303-307.

Soumen G, Sharmistha G, Ambarish M. 1997. Effect of air pollution on foliar and pollen characters of Lantana camara L, Var aculeata (L.) moldenke and Clerodendrum viscosum Vent, Environment and Ecology 15(4), 792-796.

Tarfaya M. 2005. Distribution et évaluation de la pollution atmosphérique par les NOX dans la région de Annaba, Mémoire de magistère, Université de Annaba (Algérie), 128p.

Tlili N. 2010. Bio indication végétale de pollution atmosphériquedans la régiond’Annaba (comparaison  avec les données du réseau de surveillance de la qualité de l’air SAMA SAFIA). Thèsede  Doctorat, Université Badji Mokhtar, Annaba (Algérie), 230 p.

Ward N. 1990. Multielement contamination of Britsh motorway environment. The science of the total environment, 93, 393-401.

Wolters JB, Martens MJM. 1987. Effects of air pollutants on pollen. The botanical review, 53, p.372-414.

Woodbury PB, Hudler GW. 1994. Chronic ozone exposurealters the growth of leaves, stems and roots of hybrid Populus. Environmental Pollution 85, 103-108.