The genetic diversity of Convolvulus L. species inhabited in Pakistan based on intergenic spacer trnL-trnF

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Research Paper 01/09/2017
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The genetic diversity of Convolvulus L. species inhabited in Pakistan based on intergenic spacer trnL-trnF

Shazia Kousar, Anjum Perveen, Ishrat Jamil, Syeda Qamarunnisa
J. Bio. Env. Sci.11( 3), 143-150, September 2017.
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Chloroplast non-coding regions are now frequently used in plant phylogenetic studies as they exhibit more genetic variations. The resolving power of an intergenic spacer region trnL-trnF was evaluated on Convolvulus species. Genus Convolvulus L. has many economic advantages. Genus specific primer set was designed and used for polymerase chain reaction and bi-directional Sanger sequencing. 15 ingroup and 2 outgroup species were used for the present study. A consensus tree based on Bayesian inference method was reconstructed to observe Convolvulus L. species associations. Results revealed two distinct lineages. The sister associations between C. acanthocladus and C. pseudocantabricus; C. arvensis and C. kotschyanus; C. spinosus, C. prostratus and C. scindicus are strongly supported by 1 posterior probability value. Another clade representing C. lineatus, C. cephalopodus, C. fruticosus, C. glomeratus and C. rhyniospermus also establishing close relationship between species included in the clade. The morphological data also agree the association of C. glomeratus and C. rhyniospermus. The study can help in many aspect of agronomical, medicinal and economical value of represented species in future studies.


Abbas H, Mohammad Q. 2011. Convolvulus scindicus: Conservation assessment and strategies to avoid extirpation. Pakistan Journal of Botany  43, 1685-1690.

Agarwal P, Bhawna S, Sanjay KJ, Amreen F, Shashi A. 2014. Effect of Convolvulus pluricaulis Chois. On blood glucose and lipid profile in streptozocin induced diabetic rats. International journal of Pharmaceutical Science and Research 5, 213-219.

Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ.1990. Basic local alignment search tool. Journal of Moecular Biology 215, 403-410.

Andreas W, Dietmar Q, Anna-Magdalena B, Cornelia L, Khidir WH, Thomas B. 2007. Phylogeny of basal Eudicots: Insights from non-coding and rapidly evolving DNA. Organisms Diversity & Evolution 7, 55-77.

Austin DF, Ghazanfar S. 1979. Convolvulaceae. In: Nasir, E. Ali SI (Eds) Flora of Pakistan 126: Convolvulaceae. Department of Botany, University of Karachi 1-64.

Austin DF. 1998. Parallel and Convergent Evolution in the Convolvulaceae. In: Mathews P, Sivadasan M. (Eds) Diversity and Taxonomy of Tropical Flowering Plants. Mentor Books, Calicut 201-234.

Bayer RJ, Christopher FP, Scot AK. 2000. Phylogeny of South African Gnaphalieae (Asteraceae) based on two noncoding Chloroplastsequences. American Journal of Botany 87, 259-272.

Bentham G, Joseph DH.1873. Genera Plantarum 2, 865-881.

Bhowmik D, Kumar KPS, Shravan P, Shweta S, Akhilesh PDY, Amitsankar D. 2012. Traditional Indian herbs Convolvulus pluricaulis and its medicinal importance. Journal of Pharmacognosy and Phytochemistry 1, 44-51.

Boissier E. 1875a. Plantarum Orientalium novarumdecas prima. H. Georg, Geneva, Basle and Lyons 1-8.

Boissier E. 1875b. Convolvulaceae. In: Boissier, E. (Ed.) Flora Orientalis 4: Corolliflorae and Monochlamydeae. H. Georg, Geneva, Basle and Lyons 84-123.

Brian JS, Leonard L. 2015. Nutritive Value of Field Bindweed (Convolvulus arvensis) Roots as a Potential Livestock Feed and the Effect of Aceria malherbae on Root Components. Weed Technology 29, 329-334.

Brown R. 1810. Prodromus Florae Novae Hollandiaeet Insula van Diemen. Johnson & Co, London 1-445.

Canbolat O. 2012. Potential nutritive value of field bindweed (Convolvulus arvensis L.) harvested at three different maturity stages. Kafkas Universitesi Veteriner Fakultesi Dergisi 18, 331-335.

Carine MA, Alexander JM, Russell SJ. 2003. Evolution of spines and the taxonomic status of Convolvulus section Acanthocladi: preliminary results from the ITS 2 region of nrDNA. Bocconea 16, 703-710.

Carine MA, Stephen JR, Arnoldo SG, Javier FO. 2004. Relationships of island and continental floras: molecular evidence for multiple colonization into Macaronesia and subsequent back-colonization of the continent in Convolvulus L. American Journal of Botany 91, 1070-1085.

Choisy, JD.1845. Convolvulaceae. In a. Decandole [Ed.], Prodromus 9, 323-465.

Corpet F. 1988. Multiple sequence alignment with hierarchical clustering. Nucleic Acids Research16, 10881-10890.

Darriba D, Taboada GL, Doallo R, Posada D. 2012. jModel Test 2: more models, new heuristics and parallel computing. Nature methods 9, 772-772.

Doebley J. 1992. Molecular systematic of plants and crop evolution. In: Soltis, P.S., D.E. Soltis and J.J. Doyle, Eds. Molecular systematics of plants. New York, NY, USA: Chapman Hall 202-222.

Doyle JJ, Doyle JL. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19, 11-15.

Drummond AJ, Andrew R. 2007. BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evolutionary Biology 7, 214-222.

Hallier HJG. 1893. Versuch einer natürlichen Gliederung der Convolvulaceae. Botanical Journal Arboretum 16, 479-591.

Hyosig W, Susanne SR. 2005. The Chloroplast trnL–trn F Region in the Seed Plant Lineage Gnetales. Journal of Moecular Evolution 61, 425-436.

Kelchner SA. 2000. The Evolution of non-coding Chloroplast DNA and its application in Plant Systematics. Annals of the Missouri botanical garden 87, 482- 498.

Kousar S, Qamarunnisa S, Parveen A, Jamil I. 2016. Intraspecific relationship within the genus Convolvulus L. Inferred by rbcl gene using different phylogenetic approaches. Pakistan Journal of Botany 48, 2025-2030.

Kousar S, Qamarunnisa S, Perveen A, Jamil I. 2016. Morphological features and relationship of genus Convolvulus L. (Convolvulaceae) from Pakistan. Journal of Agri-Food and Applied Sciences 4, 7-12.

Mabberley. 2008. The plant books-a portable dictionary of the vascular plants. Cambridge University press PP. 213-214.

Mitchell TC, Bethany RMW, John RIW, David JH, Robert WS, Carine MA. 2016. How the temperate world was colonized by bindweeds: biogeography of the Convolvuleae (Convolvulaceae). BMC Evolutionary Biology 16. DOI: 10.1186/s12862-016-0591-6.

Qureshi R, Raza BG, Rabia AM. 2010. Ethnomedicinal uses of herbs from northern part of Nara desert, Pakistan. Pakistan Journal of Botany 42, 839-851.

Qureshi R, Raza BG. 2008. Short report (Ethno) Ethnobotany of plants used by the Thari people of Nara Desert, Pakistan. Fitoterapia 79, 468-473.

Sa’ad F. 1967. The Convolvulus species of the Canary Isles, the Mediterranean region, and the near and Middle East. Mededelingen van het Botanisch Museum en Herbarium van de Rijksuniversite Utrecht. Bronder Offset, Rotterdam 281, 1-287.

Stefanovic S, Krueger L, Olmstead RG. 2002. Monophyly of the Convolvulaceae and circumscription of their major lineages based on DNA sequences of multiple chloroplast loci. American Journal of Botany 89, 1510-1522.

VanderpoortenA, Jonathan S. 2014. The application of molecular data to the Phylogenetic delimitation of species in Bryophytes: a note of caution. Phytotaxa 9, 229-237.

Web PB. 1841. Miscellaneous notices no 152. Botanical Register 27, 69-71.

Williams BRM, John RIW, David JH, Robert WS, Carine MA. 2014. Integrating DNA barcode data in a monograph of Convolvulus L. Taxon 63, 1287-1306.

Wood JR, Williams BRM, Thomas CM, Carine MA, David JH, Robert WS. 2015. A foundation monograph of Convolvulus L. (Convolvulaceae). PhytoKeys 18, 1-282.

Yang FS, Xiao-Quan W. 2007. Extensive length variation in the cpDNA trnL-trn F region of hemiparasitic Pedicularis and its phylogenetic implications. Plant Systematics and Evolution 264, 251-264.