The proteome response of barley root (Hordeum vulgare L.) to cold stress
Paper Details
The proteome response of barley root (Hordeum vulgare L.) to cold stress
Abstract
Low temperature limits distribution and productivity of plants, and causes genetic, morphologic and physiologic changes. The aim of this research is to investigate the cold stress effect of 4°C on morphological traits and proteome profile of root in a cold tolerant barley cultivar (EC83-1215). For this purpose, the seeds were grown in greenhouse, and the cold stress was imposed at seedling stage. The root morphological characteristics and proteome profile were examined, after 48 hours of imposing cold stress and compared with plants grown under 25°C as control. Two-dimensional electrophoresis (2-DE) was employed for proteomic analysis and total protein was extracted using TCA-acetone method. The first dimension of the electrophoresis performed as iso-electric focusing in tube gels and the second dimension carried out using slab sodium dodecyl sulfate polyacrylamide gels. Examination of the 2-DE gels showed that among 72 repeatable protein spots, 15 proteins indicated significant changes in which, 10 and 5 protein spot were up-and down-regulated, respectively. Statistical analysis revealed that applying 4°C cold stress on plants did not change the root related traits including number of roots, root fresh weight, root volume, root surface and root diameter, statistically. Therefore, lower temperatures may be needed if we wish to see any changes in these traits.
Abbasi FM, Komatsu S. 2004. A proteomic approach to analyze salt-responsive proteins in rice leaf sheath. Proteomics 7, 2072-2081.
Alizadeh A. 2004. Soil, water, plant relation-ship. Emam Reza University Press.
Bae MS, Cho EJ, Choi EY, Park OK. 2003. Analysis of the Arabidopsis nuclear proteome and its response to cold stress. The Plant Journal 36, 652-663.
Bates LS, Waldren RP, Teare ID. 1973. Rapid determination of free proline for water-stress studies. Plant and soil 39, 205-207.
Baum M, Grando S, Ceccarelli S, Backes G, Jahoor A. 2004. Localization of quantitative trait loci for dryland characters in barley by linkage apping. Challenges and Strategies of Dryland Agriculture, CSSA Special Publication 32, 191-202.
Beck EH, Heim R, Hansen J. 2004. Plant resistance to cold stress: mechanisms and environmental signals triggering frost hardening and dehardening. Journal of Biosciences 29, 449-459.
Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry 72(1), 248-254.
Ganjeali A, Palta J, Turner NC. 2008. Effects of water logging on root and shoot growth of chickpea genotypes [Cicer drietinum L.]. Gorgan University of Agricaltural Sciences and Natural Resources 15, 78-89.
Ghosh D, Xu J. 2014. Abiotic stress responses in plant roots: a proteomics perspective. Frontiers in plant science 5:6. doi: 10.3389/fpls.2014.00006.
Hajabbasi MA. 2010. Tillage effects on soil compactness and wheat root morphology. Journal of Agricultural Science and Technology 3, 67-77.
Harrak H, Azelmat S, Baker EN, Tabaeizadeh Z. 2001. Isolation and characterization of a gene encoding a drought-induced cysteine protease in tomato (Lycopersicon esculentum). Genome 44, 368-374.
Hashimoto M, Komatsu S. 2007. Proteomic analysis of rice seedlings during cold stress. Proteomics 7, 1293-1302.
Hashimoto M, Toorchi M, Matsushita K, Iwasaki Y, Komatsu S. 2009. Proteome analysis of rice root plasma membrane and detection of cold stress responsive proteins. Protein and peptide letters 16, 685-697.
Kim JS, Jung HJ, Lee HJ, Kim K, Goh CH, Woo Y, Kang H. 2008. Glycine-rich RNA-binding protein7 affects abiotic stress responses by regulating stomata opening and closing in Arabidopsis thaliana. The Plant Journal 55, 455-466.
Lee DG, Ahsan N, Lee SH, Lee JJ, Bahk JD, Kang KY, Lee BH. 2009. Chilling stress-induced proteomic changes in rice roots. Journal of Plant Physiology 166, 1-11.
Lu B, Yuan Y, Zhang C, Ou J, Zhou W, Lin Q. 2005. Modulation of key enzymes involved in ammonium assimilation and carbon metabolism by low temperature in rice (Oryza sativa L.) roots. Plant science 169, 295-302.
Mauro S, Dainese P, Lannoye R, Bassi R. 1997. Cold-resistant and cold-sensitive maize lines differ in the phosphorylation of the photosystem II subunit, CP29. Plant physiology 115, 171-180.
Mousavi A, Hotta Y. 2005. Glycine-rich proteins. Applied biochemistry and biotechnology 120, 169-174. O’Farrell PH. 1975. High resolution two-dimensional electrophoresis of proteins. Journal of biological chemistry 250, 4007-4021.
Parker R, Flowers TJ, Moore AL, Harpham NV. 2006. An accurate and reproducible method for proteome profiling of the effects of salt stress in the rice leaf lamina. Journal of Experimental Botany 57, 1109-1118.
Poustini K, Siosemardeh A, Ranjbar M. 2007. Proline accumulation as a response to salt stress in 30 wheat (Triticum aestivum L.) cultivars differing in salt tolerance. Genetic Resources and Crop Evolution 54, 925-934.
Pradet A, Raymond P. 1983. Adenine nucleotide ratios and adenylate energy charge in energy metabolism. Annual Review of Plant Physiology 34, 199-224.
Roberts JK, Aubert S, Gout E, Bligny R, Douce R. 1997. Cooperation and competition between adenylate kinase, nucleoside diphosphokinase, electron transport, and ATP synthase in plant mitochondria studied by 31P-nuclear magnetic resonance. Plant physiology 113, 191-199.
Sunkar R, Bartels D, Kirch HH. 2003. Overexpression of a stress-inducible aldehyde dehydrogenase gene from Arabidopsis thaliana in transgenic plants improves stress tolerance. The Plant Journal 35, 452-464.
Wang Y, Kim SG, Kim ST, Agrawal GK, Rakwal R, Kang KY. 2011. Biotic stress-responsive rice proteome: An overview. Journal of plant biology 54, 219-226.
Xing T, Ouellet T, Miki BL. 2002. Towards genomic and proteomic studies of protein phosp-horylation in plantpathogen interactions. Trends in plant science 7, 224-230.
Zhu J, Dong CH, Zhu JK. 2007. Interplay between cold-responsive gene regulation, metabolism and RNA processing during plant cold acclimation. Current opinion in plant biology 10, 290-295.
Mahta Mohamadiaza, Mahmoud Toorchi (2015), The proteome response of barley root (Hordeum vulgare L.) to cold stress; JBES, V7, N6, December, P153-161
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