The response of soybean growth parameters to different light intensities under cold stress conditions

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Research Paper 01/02/2015
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The response of soybean growth parameters to different light intensities under cold stress conditions

Maryam Jenabiyan, Hemmatollah Pirdashti, Yasser Yaghoubian
J. Bio. Env. Sci.6( 2), 99-106, February 2015.
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The present experiment designed to evaluate the response growth characteristics of two soybean cultivars (032 and BP) to cold stress (5 °C) at two light intensity levels [normal (8000 lux) or low light (2000 lux) intensity]. Treatments were arranged in a factorial experiment based completely randomized design with three replicates under controlled conditions. The results showed that root volume and nodule number of root in soybean seedlings significantly decreased (38 and 32 %, respectively) when grown at cold stress as compared to those grown under control conditions. Cold stress, also, reduced leaf and root fresh and dry weights and shoot dry weight in both light intensities, however, this effect was more noticeable at low light intensity for root dry weight (53%) and at normal light intensity for leaf and shoot fresh and dry weights (47% ). Cold stress, also, significantly suppressed stem fresh weight in both cultivars (34% in 032 and 27% in BP). BP cultivar when grown at 8000 lux had nearly 16% lower leaf dry weight than plants grown at, 2000 lux. However, 032 cultivar in normal light intensity showed no significant response in growth parameters to low light intensity. In both light intensities, cold stress significantly reduced the root fresh and dry weights. Although two soybean cultivars showed slightly contrast behaviors, the rate of cold stress damages decreased when soybean plants grown under lower light intensity.


Aghaee A, Moradi F, Zare-Mavian H, Zarinkamar F, Pour Irandoost H, Sharifi P. 2011. Physiological responses of tow rice (Oryza sativa L.) genotypes to chilling stress at seedling stage. African Journal of Biotechnology 10, 7617- 7621.

Allen DJ, Ort RD. 2001. Impacts of chilling temperatures on phosynthesis in warm-climate plants. Trends in Plant Science 6, 36-41.

Balestrasse KB, Tomaro ML, Batlle A, Noriega GO. 2010. The role of 5 aminolevulinic acid in the response to cold stress in soybean plants. Phytochemistry 71, 2038-2045.

Byrd GT, Ort DR, Ogren WL. 1995. The Effects of Chilling in the Light on Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Activation in Tomato (Lycopersicon esculentum Mill.). Journal of Plant Physiology 107, 585-591.

Caulfield F, Bunce JA. 1988. Comparative responses of photosynthesis to growth temperature in soybean (Glycine max [L.] Merr) cultivars. Canadian Journal of Plant Science 68, 419-425.

Gass T, Schori A, Fossati A, Soldati A, Stamp P. 1996. Cold tolerance of soybean (Glycine max (L.) Merr.) during the reproductive phase. European Journal of Agronomy 5(1-2), 71-88. DOI: 10.1016/s1161-0301(96)02011-4

Gorbani A, Zarinkamar F, Fallah A. 2011. The effect of cold stress on the morphologic and physiologic characters of two rice varieties in seedling stage. Journal of Cell &Tissue 2, 235-244.

Jafari SR, Manuchehri kalantari KH, Turkzadeh M. 2006. The evaluation of paclobutrazol effects on increase cold hardiness in tomato seedling (lycopersicume sculentum l.). Iranian Biotechnology Journal 19, 290-298.

Jasińska Z, Kotecki A. 1993. Soja. In: Rośliny strątczkowe, ed. by M. Skupińska, PWN, Warszawa 146-167 (in Polish).

Khan AZ, Khan H, Ghoneim A, Khan R, Ebid A. 2007. Seed quality and vigor of soybean as influenced by planting date, density and cultivar under temperature environment. International Journal of Agricultural Research 2, 368-376

Koster KL, Leopold AC. 1988. Sugars and desiccation tolerance in seeds. Journal of Plant Physiology 88, 829-832. DOI: 10.1104/pp.88.3.829

Lee CB, Hayashi H, BYM. 1997. Stabilization by glycine betaine of photosynthetic oxygen evolution by thylakoid membranes from Synechococcus PCC7002. Molecular Cell 7, 296-299.

Lee DG, Ahsan N, Lee SH, Kang KY, Lee JJ, Lee BH. 2007. An approach to identify cold-induced low-abundant proteins in rice leaf. Comptes Rendus Biologies 330(3), 215-225. DOI: 10.1016/j.crvi.2007.01.001

Lee MO, Kim KP, Kim B, Hahn JS, Hong CB. 2009. Flooding stress-induced glycine-rich RNA-binding protein from Nicotiana tabacum. Molecules and Cells 27(1), 47-54. DOI: 10.1007/s10059-009-0004-4

Martin B, Ort DR. 1985. The recovery of photosynthesis subsequent to chilling exposure. Photosynthesis Research 6, 121-132.

Moosavi G, Seghatoleslami MJ, Moazeni A. 2012. Effect of planting date and plant density on morphological traits, LAI and forage corn (Sc. 370) yield in second cultivation. International Research Journal of Applied and Basic Sciences 3, 57-63.

Nayyar H, Bains T, Kumar S. 2005. Low temperature induced floral abortion in chickpea: Relationship with abscisic acid and cryoprotectants in reproductive organs. Environmental and Experimental Botany 53, 39-47. DOI:10.1016/j.envexpbot.2004.02.011

Noriega G, Cruz DS, Batlle A, Tomaro M, Balestrasse K. 2012. Heme oxygenase is involved in the protection exerted by jasmonic acid against cadmium stress in soybean roots. Journal of Plant Growth Regulation 31, 79-89. DOI: 10.1007/s00344-011-9221-0

Peeler TC, Naylor AW. 1988. A comparison of the effects of chilling on leaf gas exchange in pea (Pisum sativum L.) and cucumber (Cucumis sativus L.). Journal of Plant Physiology 86, 143-146.

Sadeghi H, Heidari Sharif Abad H, Hamidi A, Nour Mohammadi G, Madani H. 2014. Effect of planting management on soybean agronomic traits. Journal of Biosciences 4, 85-91.

Sheng Xiang T. 1995. Evaluation and utilization on cold tolerance of rice in china Report of an INTEGER traveling workshop on low temperature stress of rice in china and Korea. Manila 1039, Philippines 13-20.

Stępiński D. 2002. Ribonuclease activity in roots of soybean seedlings subjected to chilling stress and recovery. ACTA Physiologiae Plantarum 24(3), 297-301. DOI: 10.1007/s11738-002-0055-z

Takeoka Y, Mamun AA, Wada T, Kanj BP. 1992. Reproductive adaptation of rice to environmental stress. Japan Scientific Societies, 226 pp.

Van Heerden PDR, Kiddle G, Pellny TK, Mokwala PW, Jordaan A, Strauss AJ, Beer Md, Schlüter U, Kunert KJ, Foyer CH. 2008. Regulation of respiration and the oxygen diffusion barrier in soybean protect symbiotic nitrogen fixation from chilling-induced inhibition and shoots from premature senescence. Journal of Plant Physiology 148, 316-327. DOI: 123422

Van Heerden PDR, Krüger GHJ. 2002. Separately and simultaneously induced dark chilling and drought stress effects on photosynthesis, proline accumulation and antioxidant metabolism in soybean. Journal of Plant Physiology 159, 1077-1086. DOI: 10.1078/0176-1617-00745

Wise RR, Ort DR. 1989. Photophosphorylation after Chilling in the Light. Journal of Plant Physiology 90, 657-664. DOI: 0032-0889/89/90/0657/08/$01 .00/0

Xing W, Rajashekar CB. 2001. Glycine betaine involvement in freezing tolerance and water stress is Arabidopsis thaliana. Environmental and Experimental Botany 46(1), 21-28. DOI: 10.1016/s0098-8472(01)00078-8

Zhang F, Lynch DH, Smith DL. 1995. Impact of low root temperatures in soybean [Glycine max (L.) Merr.] on nodulation and nitrogen fixation. Environmental and Experimental Botany 35(3), 279-285. DOI: 10.1016/0098-8472(95)00017-7