The study of environment conditions on the chemical composition of medicinal species Nepeta Menthoides of Sabalan

Abstract

Given the medicinal importance of the Nepeta menthoides and to prevent natural extinction of mentioned species, it is necessary to be examined as soon as possible the amount of be at risk and the main reasons for the extinction of species using new methods and also appropriate approaches must be considered in order to develop natural habitats of species. Nepeta menthoides species is an herbaceous and perennial plant from the family of Mint that is native to Iran and it is dispersal in northwestern of Iran. Habitat of this species is at Sabalan altitudes in Shabil and Ghotoursoei in Ardabil. In this Lab, the target seeds were collected from selected habitats at the appropriate time at the end of the growing season and in the seed stage. And they were prepared after cleaning in order to planting in the following year under ideal conditions. Plowing, leveling and preparation of three pieces of land were conducted in three parts of the province (Ardabil – Meshkinshahr – Khalkhal) in the autumn of that year and then collected seeds were planted in the center greenhouse and in the separate boxes in March of that year. The plants were transferred to plastic pots in the four leaves stage and were ready for planting. In early spring, the pots were transferred to the target regions and were cultured in a randomized complete block design with three replications. Generally it can be said that the yields of essential oils under normal conditions has been variable between 1.6 to 2.5 percent so that the highest percentage of the area has been Shabil. The number of chemical composition in the stations, Khalkhal, Ardabil and Meshkinshahr have been respectively 45, 39 and 37 and the highest chemical composition has been related to 1 and 8 Cineole with 39.9 percent in Meshkinshahr station.