The study of inorganic insoluble phosphate solubilization and other plant growth promoting characteristics of indigenous Pseudomonas fluorescens bacteria of Kordan and Gonbad regions
Paper Details
The study of inorganic insoluble phosphate solubilization and other plant growth promoting characteristics of indigenous Pseudomonas fluorescens bacteria of Kordan and Gonbad regions
Abstract
This study investigated inorganic phosphate solubilization ability of Pseudomonas fluorescens bacteria isolated from Gonbad and Kordan regions as well as some of the plant growth promoting characteristics of the superior phosphate solubilizing isolates. In this study, 58 strains of Pseudomonas fluorescens bacteria were isolated from the soil and evaluated in term of phosphate solubilization ability. Among these 58 isolates, 10 strains showed the most inorganic phosphate solubilization ability. The results showed that there is a 5% level of significant difference from the point of inorganic phosphate solubilization ability. Plant growth promoting characteristics including ACC-deaminase, Auxin and siderophore production ability was studied as well and the results showed that among these ten superior strains, isolate 12, 26 and 42 in spite of having the most ability to solubilize inorganic phosphate, had more significant superiority than other strains in term of studied plant growth promoting characteristics. Totally among these 58 bacterial isolates, 84% were able to solubilize inorganic phosphate and 16% were unable. Furthermore, 70%, 100% and 80 % of the superior phosphate solubilizing bacteria respectively showed the ability to produce siderophore, Auxin and ACC-deaminase enzyme. According to the results, there is no correlation between the ability of strains to solubilize the phosphates, produce IAA, siderophore and ACC-deaminase enzyme. Altogether the bacteria isolated from Kordan soil were more able than Gonbad soil.
Alexander DB, Zubere DA. 1991. Use of Chromazurol Sulphonate reagents to evaluate siderophore production by rhizosphere bacteria. Biology and Fertility of Soils 12(1), 39-45.
Attoe OJ, Olsen RA. 1966. Factors affecting rate of oxidation in soils of elemental sulphur and that added in rock phosphate-sulphur fusions. Soil Science 101(4), 317-325.
Barber SA. 1995. Soil Nutrient Bioavailability: A Mechanistic Approach. Ed 2. New York: John Wiley & Sons.
Chen YP, Rekha PD, Arun AB, Shen FT, Lai WA and Young CC. 2006. Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities. Applied Soil Ecology 34, 33-41.
Hameeda B, Rupela OP. 2006. Application of plant growth-promoting bacteria associated with composts and macrofauna for growth promotion of Pearl millet (Pennisetum glaucum L.). Biology and Fertility of Soils 43(2), 221-227.
Hu XC, Boyer GL. 1996. Siderophore-Mediated aluminum uptake by Bacillus megaterium ATCC 19213. Applied and Environmental 62(11), 4044– 4048.
Jalili F, Khavazi K, Pazira E, Nejati A, Asadi Rahmani H, Rasuli H & Miransari M. 2009. Isolation and characterization of ACC deaminase producing fluorescent pseudomonads, toalleviate salinity stress on canola (Brassica napus L.) growth. Journal of Plant Physiology 166, 667-674.
Keneni A, Assefa F and Prabu PC. 2010. Isolation of Phosphate Solubilizing Bacteria from the Rhizosphere of Faba Bean of Ethiopia and Their Abilities on Solubilizing Insoluble Phosphates. Journal of Agricultural Science and Technology 12, 79-89.
Khakipour N, Khavazi K, Pazira E & Asadirahmani H. 2008. Production of Auxin Hormone by fluorescent Pseudomonads. American-Eurasian J Agric & Environ Sci 4(6), 687-692.
Khan M S, Zaidi A, Wani P A and Oves M. 2008. Role of plant growth promoting rhizobacteria in the remediation of metal contaminated soils. Environmental Chemistry Letters 7, 1-19.
Kim J W, Ryu S H, Rhee S G. 1989. Cyclic and noncyclic inositol phosphates are formed at different ratios by phospholipase C isozymes. Biochemical and Biophysical Research Communications 163, 177-182.
Nahas E. 1996. Factors determining rock phosphate solubilization by microorganisms isolated from soil. World Journal of Microbiology and Biotechnology 12, 567-572.
Pandey A, Trivedi P, Kumar B, Palni LMS. 2006. Characterization of a phosphate solubilizing and antagonistic strain of Pseudomonas putida (B0) isolated from a Sub-Alpine location in the Indian Central Himalaya. Current microbiology 53, 102–107.
Patten CL, Glick BR. 2002. Role of Pseudomonas putida indole acetic acid in development of host plant root system. Applied and Environmental Microbiology 68(8), 3795-3801.
Schaad NW, Jones JB, Chun W. 2001. Laboratory guide for identification of plant pathogenic bacteria: Aps Press Minnesota. 373pp.
Schwyn B, Neilands J B. 1987. Universal chemical assay for the detection and determination of siderophores. Analytical biochemistry 160, 47–56.
Shaharoona B, Naveed M, Arshad M & Zahir ZA. 2008. Fertilizer-dependent efficiency of Pseudomonads for improving growth, yield, and nutrient use efficiency of wheat (Triticum aestivum L.). Applied Microbiology and Biotechnology 79, 147– 155.
Soltani A, Saleh Rastin N, Khavazi K, Asadi Rahmani H, Abbaszadeh P. 2007. Isolation and determination of PGP characteristics in some of indigenous fluorescent Pseudomonads of Iranian soils. Iranian Journal of Soil and Waters Sciences 21(2), 289-277.
Sperber JI. 1958. The incidence of apatite solubilizing organisms in the rhizosphere and soil. Crop and Pasture Science 9, 778-781.
Srideve M, and Mallaiah KV. 2007. Bioproduction of indole acetic acid by Rhizobium strains isolated from root nodules of green manure crop, Sesbania sesban (L.) Merr. Iranian Journal of Biotechnology 5(3), 178-182.
Vassilev A, Vangronsveld J and Yordanov I. 2002. Cadmium phytoextraction: Present state, biological backgrounds and research needs. Bulg J Plant Physiol 28(3–4), 68–95.
Farshad Alishahi, Hossein Ali Alikhani, Ahmad Heidari, Leila Mohammadi, 2013. The study of inorganic insoluble phosphate solubilization and other plant growth promoting characteristics of indigenous Pseudomonas fluorescens bacteria of Kordan and Gonbad regions. Int. J. Agron. Agric. Res., 3(12), 53-60.
Copyright © 2013 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.