Total flavonoids, alkaloids, antioxidant assay and preliminary screening of secondary metabolites in root extract of some shrubs
Paper Details
Total flavonoids, alkaloids, antioxidant assay and preliminary screening of secondary metabolites in root extract of some shrubs
Abstract
Biochemically active constituents of plants are crucial and contribute to increase the plant`s economic and medicinal values. Different secondary metabolites such as alkaloids, flavonoids, phenols and quinones protect the plants and also consumed as traditional health remedies. Furthermore, plant extracted secondary metabolites are being utilized in modern drugs manufacturing. The current study was carried to find out the preliminary screening of secondary metabolites, total flavonoids, total alkaloids content and antioxidant potential of roots extract. The result showed that more flavonoids content was found Capparis deciduas (22.02mg QE/g DW) followed by Withania somnifera (17.21mg QE/g DW), Salsola imbricata (12.36mg QE/g DW) and least content was detected in Carotalaria burhia (07.46mg QE/g DW) root extract. Total alkaloids content was found more in Capparis deciduas (28.78mg/100g) following by Withania somnifera (23.03mg/100g) Carotalaria burhia (19.67mg/100g) and Salsola imbricata (18.98 mg/100g) respectively. Root extract of selected plant species revealed substantial antioxidant potential in following order: Capparis deciduas (27.08%)> Withania somnifera (24.42%)> Salsola imbricata (17.06%)> Carotalaria burhia (13.46%).
Abdalrahman AA, El Tigani S, Yagi S. 2016. Biological activity of extracts from Capparis decidua L. twigs. Journal of Medicinal Plants Research 10(1), 1-7.
Ajanal M, Gundkalle MB, Nayak SU. 2012. Estimation of total alkaloid in Chitrakadivati by UV-Spectrophotometer. Ancient science of life 31(4), 198.
Akhtar N, Mirza B. 2015. Phytochemical analysis and comprehensive evaluation of antimicrobial and antioxidant properties of 61 medicinal plant species. Arabian Journal of Chemistry.
Ali SS, Kasoju N, Luthra A, Singh A, Sharanabasava H, Sahuand A, Bora U. 2008. Indian medicinal herbs as source of antioxidants. Food Res. Int 41, 1-15.
AV, Rao TV, Sundararaju D, Vanisree M, Tsay HS, Subbaraju GV. 2005. Assessment of bioactivity of Indian medicinal plants using brine shrimp (Artemia salina) lethality assay. Int J Appl Sci Eng 3(2), 125-34.
Bhattacharya SK, Bhattacharya D, Sairam K. Ghosal S, 2002. Effect of Withania somnifera glycowithanolides on a rat model of tardive dyskinesia. Phytomedicine 9(2), pp.167-170.
Bibi G, Haq I, Ullah N, Mannan A, Mirza B. 2011. Antitumor, cytotoxic and antioxidant potential of Aster thomsonii extracts. Afr. J. Pharm. Pharmacol 5, 252-58.
Breslin, Andrew. 2017. The Chemical Composition of Green Plants. Sciencing, Leaf Group Ltd.
Ch S, Venkateshwar C, Gangadhar, Rao S. 2014. Preliminary phytochemical studies of medicinal plant drug: Withania somnifera Linn.
Chang CC, Yang MH, Wen HM, Chern JC. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J. Food Drug Anal 10, 178-182.
Chopra RN, Nayar SL, Chopra IC. 2006. Glossary of Indian Medicinal Plants. National Institute of Science Communication and Information Resources, New Delhi p. 80.
Cybulska I, Brudecki G, Alassali A, Thomsen M, Brown JJ. 2014. Phytochemical composition of some common coastal halophytes of the United Arab Emirates. Emirates Journal of Food and Agriculture 26(12), 1046.
Dalziel JM. 1948. Useful Plants of West Tropical Africa. Crown Agents for the Colonies, London pp. 178-180.
Elmas Özeker. 1999. Phenolic Compounds and Their Importance. Anadolu J of Aari. 9, 114-124. 20.
Han X, Shen T, Lou H. 2007. Dietary polyphenols and their biological significance. International Journal of Molecular Sciences 8(9), 950-988.
Harborne AJ. 1998. Phytochemical methods a guide to modern techniques of plant analysis. Springer science & business media.
Harborne J. 1973. Phytochemical methods, A guide to modern techniques of plant analysis, JB Harborne. Chapman. London GB.
Kataria S, Shrivastava B, Khajuria RK, Suri KA, Sharma P. 2010. Antimicrobial activity of Crotalaria burhia Buch.-Ham. roots. Ind. J. Nat. Prod. Resour 1, 481-484.
Khalid M, Siddiqui HH. 2011. Pharmacognostical evaluation and qualitative analysis of Saccharum spontaneum (L.) root. International journal of pharmaceutical sciences and drug research 3(4), 338-341.
Khalighi-Sigaroodi F, Ahvazi M, Hadjiakhoondi A, Taghizadeh M, Yazdani D, Khalighi-Sigaroodi S, Bidel. S.2012. Cytotoxicity and antioxidant activity of 23 plant species of Leguminosae family. Iranian journal of pharmaceutical research: IJPR 11(1), 295.
Krishnaraju AV, Rao T V, Sundararaju D, Vanisree M, Tsay HS, Subbaraju GV. 2005. Assessment of bioactivity of Indian medicinal plants using brine shrimp (Artemia salina) lethality assay. Int J Appl Sci Eng 3(2), 125-34.
Makky EA, Mashitah MY, Ibrahim MM. 2012. Impact of medicinal plants phytocomponents against antibiotic resistant bacteria. Journal of Chemical and Pharmaceutical Research 4(1), 881-893.
Mishra LC, Singh BB, Dagenais S. 2000. Scientific basis for the therapeutic use of Withania somnifera (Ashwagandha): A review-Alternative medicine review 5(4), 334-346.
Molyneux RJ, Lee ST, Gardner DR, Panter KE, James LF. 2007. Phytochemicals: The good, the bad and the ugly. Phytochemistry 68(22), 2973-2985.
Moza PN, Singh J. 1967. A simple method for estimation of total alkaloids in Ashwagandha, J. Res. IND. Med 2(1), 77-78.
Munir U, Perveen A, Qamarunnisa S. 2014. Comparative Pharmacognostic evaluation of some species of the genera Suaeda and Salsola leaf (Chenopodiaceae). Pakistan Journal of Pharmaceutical Sciences 27(5).
Okwu DE. 2004. Phytochemicals and vitamin content of indigenous spices of South Eastern Nigeria. J. Sustain. Agric. Environ 6(1), 30-37.
Pattanaik C, Reddy CS, Dhal NK, Das R. 2006. Some phytotherapeutic claims by tribals of Rayagada District, Orissa, India. Ethnobot. Leaflets 10, 189-197.
Pourmorad F, Hosseinimehr SJ, Shahabimajd N. 2006. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. African journal of biotechnology 5(11).
Rathee S, Rathee P, Rathee D, Rathee D, Kumar V. 2010. Phytochemical and pharmacological potential of kair (Capparis decidua). International Journal of Phytomedicine 2(1).
Roopashree TS, Dang R, Rani RS, Narendra C. 2008. Antibacterial activity of antipsoriatic herbs: Cassia tora, Momordica charantia and Calendula officinalis. International Journal of Applied Research in Natural Products 1(3), 20-28.
Savita S, Rao DV, Sharma RA. 2010. Phytochemical evaluation and quantification of primary metabolites of Maytenus emarginata (Willd.) Ding Hou. Journal of Chemical and Pharmaceutical Research 2(6), 46-50.
Shah BA, Qazi GN, Taneja SC. 2009. Boswellic acids: a group of medicinally important compounds. Natural product reports 26(1), 72-89.
Shekhawat N, Vijayvergia R. 2010. Evaluation of antimicrobial potential of some medicinal plants against plant and human pathogens. J. Pharm. Res 3(4), 700-702.
Soni B. 2014. Preliminary phytochemical screening and antimicrobial activity of methanol extract of Crotalaria burhia. Pharma-Tutor 2(9), 115-118.
Zia-Ul-Haq M, Ćavar S, Qayum M, Imran I, Feo VD. 2011. Compositional studies: antioxidant and antidiabetic activities of Capparis decidua (Forsk.) Edgew. International journal of molecular sciences 12(12), 8846-8861.
Muzafar Iqbal, Muhammad Nadeem, Shaista Jabeen, Noreen Fatima, Shagufta Parveen (2017), Total flavonoids, alkaloids, antioxidant assay and preliminary screening of secondary metabolites in root extract of some shrubs; IJB, V11, N5, November, P270-276
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