Total phenolic contents of two varieties of Crocus sativus and their antioxidant activity

Paper Details

Research Paper 01/03/2019
Views (582)
current_issue_feature_image
publication_file

Total phenolic contents of two varieties of Crocus sativus and their antioxidant activity

Zafar Iqbal, Mohsan Javed, Samina Gull, Mian Habib-Ur-Rahman Mahmood, Zhang Hai
Int. J. Biosci. 14(3), 423-427, March 2019.
Copyright Statement: Copyright 2019; The Author(s).
License: CC BY-NC 4.0

Abstract

Methanolic extract of two varieties of crocus sativus dried stigmas was taken for total phenolic contents and antioxidant activity by using 1,1-diphenyl-2-picryl hydroxide scavenging. UV absorption of both samples separately showed three maximum wavelengths 236 nm and 234 nm (maximum absorbance of picrococine), 320 nm and 308 nm (maximum absorbance of safranal) and 436 nm and 434 nm (maximum absorbance of crocine) respectively. The electrochemical studies showed the oxidation reduction peaks. Total Phenolic content of red and brown saffron by using FC reagent was 5.05 mg and 4 mg of gallic acid equivalent/g of saffron extract respectively. At 50 µL concentration, both red and brown crocus sativus showed 71.68% and 73.70% inhibition respectively.

Basker D, Negbi M. 1999. The uses of saffron.Economic Botany 3, 228-236.

Espin JC, Soler-rivas C, Wichers HJ. 2000. Characterization o the total free radical scavenging capacity of vegetable oils and oil fractions using 2,2-diphenyl-1-picrylhydrazyl radical. Journal of Agriculture and Food Chemistry 48, 648-656.

Grilli CM. 2004. Saffron reproductive biology.Acta Horticulture 650, 25-37.

Inayat A, Khan SR, Waheed A, Deeba F. 2010. Applications of eco-friendly natural dyes on leather using different modrants. Proceeding Pakistan Academy Science 47, 131-135.

Karimi E, Oskoueian E, Hendra R, Jaafar HZE. 2010. Evaluation of Crocus sativus L. stigma phenolic and flavonoid compounds and its antioxidant activity. Molecules 15, 6244–6256.

Kasparova S, Brezova V, Valko M, Horecky J, Mlynarik V,  Liptaj T, Dobrota D. 2005. Study of the oxidative stress in a rat model of chronic brain hypoperfusion. Neutochemistry International 46, 601-61.

Learsomrong K, Suttajit M, Chantiratikul P. 2009. Flow injection analysis system for the determination of total phenolic compounds by using folin-ciocalteu assay. Asian Journal of Applied Sciences 2, 184-190.

Lozano P, Castellar M, Simancas M, Iborra L. 1999. Quantitative high-performance liquid chromatographic method to analyse commercial saffron (Crocus sativus L.) products. Journal of Chromatography 830, 477-483.

Musa AE, Madhan B, Madhulatha W, Raghava RJ, Gasmelseed GA,  Sadulla S. 2009. Coloring of leather using henna-natural alternative material for dyeing. The Journal of the American Leather Chemists Association 104, 183-190.

Onem E, Gulumser GS, Ocak B. 2011. Evaluation of natural dyeing of leather with rubiatinctorum Extract. Ekoloji Dergisi 20, 81-87.

Ordoudi SA, Befani CD, Nenadis N, Koliakos GG, Tsimidou MZ. 2009. Further examination of antiradical properties of crocus sativus stigmas extract rich in crocins. Journal of Agriculture Food Chemistry 57, 3080–3086.

Paschal FD, Danladi AA, Myek B, Sunday DJ, Joshua I, Ferdinand N. 2015. Extraction of dyestuff from justiciacarnea hooker and its application in the dyeing of wool, leather and cotton, International Journal of Mordern Chemistry 7, 81-90.

Plessner O, Negbi M, Ziv M, Basker D. 1989. Effects of temperature on the flowering of Crocus (Crocus sativus L.): Induction of hysteranthy. Israel Journal of Botany 38,1-7.

Sanja SD, Sheth N, Patel D, Patel B. 2009. Characterization and evaluation of antioxidant activity of Portulacaoleracea. International Journal of Pharmacy and Pharmaceutical 1, 31-36.

Sivakumar V, Anna JL, Vijayeeswarri J, Swaminathan G. 2009. Ultrasound assisted enhancement in natural dye extraction from beetroot for industrial applications and natural dyeing of leather. UltrasonicsSonochemistry16, 782-789.

Nurul SR, Asmah R. 2012. Evaluation of antioxidant properties in fresh and pickled papaya. International Food Research Journal 19, 1117-1124.

Related Articles

Yield performance assessment of different mustard cultivars under field conditions

Md. Khan Jahan Ali, Md. Moshiur Rahman, Kamrun Nahar, Sharmin Ara Jannat, Mst. Khadija Khatun, Sushan Chowhan, Md. Habibur Rahman, Int. J. Biosci. 27(2), 277-282, August 2025.

Species composition of xylomicobiota of some woody plants distributed in Azerbaijan

K. F. Bakhshaliyeva, V. Y. Hasanova, N. R. Namazov, B. N. Aliyeva, S. C. Garayeva, S. E. Nagiyeva, P. Z. Muradov, Int. J. Biosci. 27(2), 267-276, August 2025.

Perception of producers and processors on sorghum diversity in the context of climate change in center and Northern Benin

Parfait Segla Alohoutade1,2,3, Alphonse Sako Avocefohoun*1,2,3, Sènan Vodouhe2, Mohamed Kanazoe1,2,3, Nicodème Chabi1, Lamine Said Baba-Moussa3, Int. J. Biosci. 27(2), 256-266, August 2025.

Implications of aberrant glycosylation on age-related disease progression

Tahmid Ahmad Patwary, Mukramur Rahman, Md. Nafis Fuad Prottoy, Sayad Md. Didarul Alam, Int. J. Biosci. 27(2), 243-255, August 2025.

Epizootic status and eradication of parasitic diseases in brown bears transferred to the rehabilitation center in Azerbaijan

Siala İ. Rustamova, Aygun A. Azizova, Gular R. Mammadova, Ramin S. Mammadov, Int. J. Biosci. 27(2), 236-242, August 2025.

Cytotoxic and apoptotic effects of Annona squamosa (Atis) crude leaf extract against A549 (Human Lung Adenocarcinoma) cell line

Ashton U. Lim, Noricel U. Garcia, Alkauzar H. Tantong, Int. J. Biosci. 27(2), 226-235, August 2025.

Perceptions of stakeholders towards sheep and goat dairy products in Benin

Yvette Adje, Philippe Sessou, Aretas Tonouhewa, Paulin Azokpota, Lamine Baba-Moussa, Souaïbou Farougou, Int. J. Biosci. 27(2), 211-225, August 2025.

Medicinal plants sold in Daloa markets: Traditional knowledge and Public health issues

Kouakou Yao Bertin, Kouakou Assoman Serge Alain, Kouame Yao Anicet Gervais, Malan Djah François, Bakayoko Adama, Int. J. Biosci. 27(2), 200-210, August 2025.