Total phenolics and total flavonoids of extracts from freshwater Clam (Corbicula fluminea) using different solvents

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Research Paper 01/04/2021
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Total phenolics and total flavonoids of extracts from freshwater Clam (Corbicula fluminea) using different solvents

Simonette C Villabeto, Romeo M Del Rosario, Oliva P Canencia
J. Bio. Env. Sci.18( 4), 93-98, April 2021.
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The ethanol, ethyl acetate, and hexane extracts of the freshwater clam (Corbicula fluminea) were studied for the total phenolics and total flavonoids. Total phenolics and total flavonoids of the extracts were evaluated using Folin-Ciocalteau and Aluminum chloride colorimetric methods respectively. The findings showed that the total phenolics of the ethanol extract (1.67±0.28mg GAE/g of dried sample) were substantially higher than the total phenolics obtained from the ethyl acetate (0.70±0.00mg GAE/g) and hexane extracts (0.56±0.23mg GAE/g). While the total flavonoids in the ethyl acetate extract displayed a slightly higher total flavonoid (43.84±0.92mg QE/g of dried sample) relative to ethanol (30.41±1.34mg QE/g of dried sample) and hexane extracts (20.28±0.00mg QE/g of dried sample). Using ethanol, the highest yield for extraction was obtained. Ethanol is the best solvent among the three – ethanol, ethyl acetate, and hexane in terms of extraction yield and total phenolics. In addition, it can be inferred that the presence of significant amounts of phenolics and flavonoids suggests that freshwater clam is a promising source of antioxidants that provides nourishing proteins and oxidative stress remedies.


Adaramola B, Onigbinde A. 2016. Effect of Extraction Solvent on the Phenolic Content, Flavonoid Content and Antioxidant Capacity of Clove Bud. IOSR Journal of Pharmacy and Biological Sciences II(3)I, 33-38.

Bag GC, Devi PG, Bhaigyabati Th. 2015. Assessment of Total Flavonoid Contents and Antioxidant Activity of Methanolic Rhizome Extract of Three Hedychium Species of Manipur Valley. Int. J. Pharm. Sci. Rev. Res 30(1), 154-159

Beecher GR. 2003.  Overview of dietary flavonoids: nomenclature, occurrence and intake. Jour Nut 133, 3248-3254.

Chijimatsu T, Tatsugushi I, Abe K, Oda H, Mochizuki S. 2008. A Freshwater clam (Corbicula fluminea) extract improves cholesterol metabolism in rats fed on a high-cholesterol diet. Bioscience Biotechnology and Biochemistry 72(10), 2566-2571.

Do QM, Ankkawijaya AE, Nguyen PLT, Huynh LH, Soetaredjo FE, Ismadji S. 2014. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatic. Journal of Food and Drug Analysis 22, 296-302.

Eswar A, Isha Z, Shanmugasundaram S, Ramamoorthy K, Nanda RK. 2015. Evaluation of preliminary qualitative analysis of Clam Paphia malabarica extracts from Girgaon chowpatty Creek, Mumbai. Journal of Pharmaceutical, Chemical and Biological Sciences 3(4), 461-468.

Hsu CL, Hsu CC, Yen GC. 2010, Hepatoprotection by freshwater clam extract against CCl4-induced hepatic damage in rats. Am. J. Chin. Med 38, 881-894.

Khedher O, Moussaoui Y, Salem RB. 2014. Solvent Effects on Phenolic Contents and Antioxidant Activities of the Echinops Spinosus and the Limoniastrum Monopetalum Research Journal of Pharmaceutical, Biological and Chemical Sciences 5(2), 66-76.

Kong ZL, Yu SC, Dai SA, Tu CC, Pan MH, Liu YC. 2011. Polyoxygenated Sterols from freshwater Clam. Helvetica Chimica Acta Vol. 94.

Koyama T, Chounan R, Uemura D, Yamaguchi K, Yazawa K. 2006. Hepatoprotective effect of a hot-water extract from the edible thorny oyster Spondylus varius on carbon tetrachloride -induced liver injyury in mice. Biosci. Biotechnol. Biochem 70(3), 729-731.

Michiels JA, Kevers C, Pincemail J, Defraigne JO, Dommes J. 2012. Extraction conditions can greatly influence antioxidant capacity assays in plant food matrices. Food Chemistry 130(4), 986-993.

Ngo TV, Scarlett CJ, Bowyer MC, Ngo PD, Vuong QV. 2017. Impact of Different Extraction Solvents on Bioactive Compounds and Antioxidant Capacity from the Root of Salacia chinensis L. Journal of Food Quality Volume 2017.

Ramasamy M, Balasubramanian U. 2012. Identification of Bioactive Compounds and Antimicrobial Activity of Marine Clam Anadara Granosa (Linn.). International Journal of Science and Nature 3(2), 263-266.

Settharaksa S, Madaka F, Sueree L, Kittiwisut  S,  Sakunpak  A,  Moton   C, Charoenchai L. 2014. Effect of Solvent Types on Phenolic, Flavonoid Contents and Antioxidant Activities of Syzygium gratum (Wight) S.N. Int J Pharm Pharm Sci 6(2), 114-116.

Tomsone L, Kruma Z, Galoburda R. 2012. Comparison of Different Solvents and Extraction Methods for Isolation of Phenolic Compounds from Horseradish Roots (Armoracia rusticana). International Journal of Agricultural and Biosystems Engineering 6(4), 236-241.