Toxicity of selected mangroves species on brine shrimp (Artemia salina)
Paper Details
Toxicity of selected mangroves species on brine shrimp (Artemia salina)
Abstract
The study aimed to evaluate the toxicity of different mangroves species Rhizophora apiculata, Rhizophora mucronata, and Bruguiera sexangula against brine shrimp (Artemia salina) as a preliminary assessment of its potential adverse effects as an herbal medicine. Brine shrimp were chosen as test organisms due to their sensitivity to various substances, making them suitable indicators for toxicity testing. The mangrove samples were extracted and were prepared to different dilutions for this study: 1 μg/ml (T1), 10 μg/ml (T2), 100 μg/ml (T3), 1000μg/ ml (T4). The findings of the study showed that the lethality of the three mangrove extracts on brine shrimp nauplii was found to be concentration-dependent. The brine shrimp mortality increases as extract concentration increases with T4 (1000 μg/ml) consistently showed higher death percentage compared to lower treatments is advised that further research be done on the Bruguiera sexangula crude extract to determine the bioactive components that are responsible for both its toxicity and its biopesticide properties.
Anderson JE, Goetz CM, McLaughlin JL, Suffness M. 1991. A blind comparison of simple bench-top bioassays and human tumour cell cytotoxicities as antitumor prescreens. Phytochemical Analysis 2, 107–111.
Bandaranayake W. 2002. Bioactivities, bioactive compounds and chemical constituents of mangrove plants. Wetlands Ecology and Management 10, 421–452. DOI: 10.1023/A:1021397624349.
Baravalia Y, Vaghasiya Y, Chanda S. 2012. Brine shrimp cytotoxicity, anti-inflammatory and analgesic properties of Woodfordia fruticosa Kurz flowers. Iranian Journal of Pharmaceutical Research 11(3), 851–861.
Ekor M. 2014. The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Frontiers in Pharmacology 4, 177. DOI: 10.3389/fphar.2013.00177.
Ginting B, Yahya M, Nurdin NN, Maulidna M. 2021. Evaluation of antioxidant and anticancer activity of Myristica fragrans Houtt. bark. Pharmacognosy Journal 13(3), 780–786. DOI: 10.5530/pj.2021.13.99.
Guevara BQ. 2005. A guidebook to plant screening: phytochemical and biological (Rev. ed.). University of Santo Tomas Pub. House.
Gupta R, Polaka S, Rajpoot K, Tekade M, Sharma MC, Tekade RK. 2022. Importance of toxicity testing in drug discovery and research. In: Tekade RK (Ed.), Advances in Pharmaceutical Product Development and Research: Pharmacokinetics and Toxicokinetic Considerations (Vol. 2, pp. 117–144). Academic Press. DOI: 10.1016/B978-0-323-98367-9.00016-0.
McLaughlin JL, Rogers LL, Anderson JE. 1998. The use of biological assays to evaluate botanicals. Drug Information Journal 32(2), 513–524.
Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE, McLaughlin JL. 1982. Brine shrimp: A convenient general bioassay for active plant constituents. Planta Medica 45(5), 31–34. DOI: 10.1055/s-2007-971236.
Mitra S, Naskar N, Chaudhuri P. 2021. A review on potential bioactive phytochemicals for novel therapeutic applications with special emphasis on mangrove species. Phytomedicine Plus 1(4), 100107. DOI: 10.1016/j.phyplu.2021.100107.
Pasion E, Tumaliuan B. 2015. State of mangroves in Cagayan. In: Salmo SG III, Favis AMT, Ting MNS (Eds.), Proceedings State of the Mangrove Summit: Northwestern Luzon Proceedings (p. 113). Ateneo de Manila University.
Quazi SS, Fatema CA, Mir M. 2017. Brine shrimp lethality assay. Division of Arsenic Research, Department of Pharmacology, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka, Bangladesh. DOI: 10.3329/bjp.v12i2.32796.
Sagrin MS, Lasano NF, Shukri R, Ramli NS. 2019. Antioxidant properties and toxicity assessment of the Crescentia cujete extracts in brine shrimp (Artemia salina). Sains Malaysiana 48(4), 831–840. DOI: 10.17576/jsm-2019-4804-15.
Salmo SG, Favis AM, Ting MN. 2014. State of the Mangrove Summit: Northern Luzon Proceedings. October 2014. ISSN 2449-4305.
Sam TW. 1993. Toxicity testing using the brine shrimp: Artemia salina. In: Colegate SM, Molyneux RJ (Eds.), Bioactive Natural Products Detection, Isolation, and Structural Determination. CRC Press, Boca Raton, FL, pp. 442–456.
Solis PN, Wright CW, Anderson MM, Gupta MP, Phillipson JD. 1993. A microwell cytotoxicity assay using Artemia salina (brine shrimp). Planta Medica 59(3), 250–252. DOI: 10.1055/s-2006-959661.
Joana Rose A. Narag-Pagaduan, Emma L. Ballad, Jr. Lito S. Guzman, 2024. Toxicity of selected mangroves species on brine shrimp (Artemia salina). J. Biodiv. Environ. Sci., 25(6), 85-94.
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