J. Bio. Env. Sci.12( 3), 370-377, March 2018
The using of immobilization between laccase and bentonite by glutaraldehyde resulted to immobilized 91% of the amount of total free enzyme, the optimum pH activity of the free and immobilized laccase was 6 and immobilized enzyme was stable between 4.5-7 and its loss about 54 and 38% from original activity at pH 3 and 8 respectively, while temperature activity of free and immobilized laccase was 40 and 45°C respectively, immobilized enzyme was stable at 60°C for 15min, and it loses about 88% of its original activity at 80°C for the same time, the results was shown that immobilized laccase retained its full activity for 22 days, but it retained 89.2% of its original activity after storage for 30 days at 4°C, also, Immobilized laccase was kept all its activity after 30 consecutive used, and after 40 consecutive used keep about 75% of its initial activity. The treatment of wastewater sample with 1gm of immobilized laccase for 10, 20, 30, 40, 50, 60min lead to remove 30.17, 62.48, 90.03, 90.12, 90.13 and 90.13% of phenol compounds respectively.
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