Use of immobilized laccase in bioremediation of phenolic compounds which causes environmental pollution
Paper Details
Use of immobilized laccase in bioremediation of phenolic compounds which causes environmental pollution
Abstract
The using of immobilization between laccase and bentonite by glutaraldehyde resulted to immobilized 91% of the amount of total free enzyme, the optimum pH activity of the free and immobilized laccase was 6 and immobilized enzyme was stable between 4.5-7 and its loss about 54 and 38% from original activity at pH 3 and 8 respectively, while temperature activity of free and immobilized laccase was 40 and 45°C respectively, immobilized enzyme was stable at 60°C for 15min, and it loses about 88% of its original activity at 80°C for the same time, the results was shown that immobilized laccase retained its full activity for 22 days, but it retained 89.2% of its original activity after storage for 30 days at 4°C, also, Immobilized laccase was kept all its activity after 30 consecutive used, and after 40 consecutive used keep about 75% of its initial activity. The treatment of wastewater sample with 1gm of immobilized laccase for 10, 20, 30, 40, 50, 60min lead to remove 30.17, 62.48, 90.03, 90.12, 90.13 and 90.13% of phenol compounds respectively.
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Mohammed Abdulrazzaq Alsoufi, 2018. Use of immobilized laccase in bioremediation of phenolic compounds which causes environmental pollution. J. Biodiv. Environ. Sci., 12(3), 370-377.
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