Variability of pollen grains in clones of Dioscorea cayenensis – D. rotundata complex grown in centre of Benin
Paper Details
Variability of pollen grains in clones of Dioscorea cayenensis – D. rotundata complex grown in centre of Benin
Abstract
The classical genetic breeding improvement of the species is through gene flows, based on pollen grains flow between individuals. The aim of this study is to evaluate the pollen variation between some clones of the complex Dioscorea cayenensis – D. rotundata of three villages of Centre of Benin. Unopened and mature male flowers were dissected and the anthers stained with acetic carmine. The preparations were observed with an optical microscope. Data on the shape, length, width of pollen were collected and subjected to descriptive analyse and pollen surface to an analysis of variance. Two forms of pollen grains were observed; the ovals shape percentage vary from 38 to 88.23%, and that of the rounds shape vary from 11.77 to 70%. The major axis has an average length of 0.49 ± 0.16 μm and that of the minor 0.32 ± 0.14 μm for oval shapes; the average diameter of round pollen was 0.36 ± 0.09 μm. These data are statistically very different (P<0.0001). The pollen grains of Dioscorea cayenensis – D. rotundata complex are oval or round shapes. Both shapes are present in all cultivars and the dominance of one shape of pollen grains is under genetic program. Size of the pollen grains is conserved within the species. These results are important for the classical genetic improvement of this vegetative species, where pollen grains would be the vehicles of interesting traits between cultivars identified in farmer’s fields.
Agriculture A. 1992. The challenge of the intermediate mechanization of food crops. Africa agriculture 199, 13–23.
Akoroda MO. 1984. ‘Estimating pollen viability for controlled hybridization in white yam.’, Crops Research (Horticultures Ressource )., 24, 11–22 p.
Arezki D, Cadeac F, Durrieu G. 1991. Study of the geographic variability of the dimensions of the Atlas cedar pollen (Cedrus atlantica Manetti) in Algeria ’, Bulletin of the French Botanical Society. Botanical Letters 3(138), p. 215–230. http://dx.doi.org/10.1080/01811797.1991.10824923.
Assaba IE. 2013. Genetic diversity and Biology of sexual reproduction of cultivated yams of the Dioscorea cayenensis – D. rotundata complex and Dioscorea dumetorum: case of the township of Dassa – Zoumé / Master thesis in Genetics, Biotechnologies and Biological Resources / University of Abomey Calavi, 69 p.
Assaba EI, Mounirou Y, Totin Vodounon HS, Quenum GA, Zoundjihèkpon J. 2015. Variability of pollen grains of the Dioscorea cayenensis – D. rotundata complex yams from the township of Dassa-Zoumé. Vth Symposium of Sciences, Cultures and Technologies / University of Abomey Calavi / September 2017.
Assaba E, Mounirou Y, Totin Vodounon H, Qunum G, Zoundjihèkpon J. 2018. Caractéristics of cultived yams of the Dioscorea cayenensis-Dioscorea rotundata complex seeds and évolution of them germination rate. Africa Science 14(4), p 195 – 208, ISSN 1813-548X, http://www.afriquescience.net
Denadi N. 2018. Attempt to improve the fruiting rate of flowering cultivars of the Dioscorea cayenensis-Dioscorea rotundata complex in the fields of producers in the township of Dassa-Zoumè and Savalou. Master thesis in Genetics, Biotechnologies and Biological Resources/ University of Abomey Calavi, 66 p.
Ducreux G, Rossignol JL, Rossignol NI. 1986. Enriched Potato’, La Recherche 6(2), p 193–203.
Dumont R, Zoundjihèkpon J, Vernier P. 2010. Origin and diversity of yams Dioscorea rotundata Poir, Agricultures Books 19(4), p 255–261. http://dx.doi.org/10.1684.
Franssen AS, Skinner DZ, Al-Khatib K, Horak MJ. 2001. ‘Pollen morphological differences in Amaranthus species and interspecific hybrids’, Weed Science Society of America, 49(6), p 732–737. ISSN: 0043-1745. http://dx.doi.org/10.1614/00431745(2001)049[0732:PMDIAS]2.0.CO;
Habari M. 1983. Morphology of the pollen grains of some anemogamous species around Kisangani (Haut-Zaire). Bachelor thesis in Plant Biology. Faculty of Sciences – University of Kisangani Zaire, p 47.
INRA. 2006. Yam: flowers for new hybrids, Seed Quest News Section. http://www.inra.fr/presse/yam
Iwanami Y, Sasakuma T, Yamada Y, 1988.‘Pollen morphology of flowering plants. Pollen: Illustrations and Scanning Electromicrographs’, Tokyo: Kodansha and Springer Verlag, Berlin Press., p 10–122.
Loko Y. 2013. Analysis of the varietal diversity of cultivated yams of the Dioscorea cayenensis Lam D. rotundata Poir complex from Benin and participatory agronomic evaluation of cultivars for their sustainable use and in situ conservation. PhD thesis of University of Abomey-Calavi, in agricultural Entomology, 315.
Loko YL, Dansi A, Tamo M, Bokonon-Ganta A. H, Assogba P, Dansi M, Vodouhè R, Akoegninou A, Sanni A. 2013. ‘Storage Insects on Yam Chips and Their Traditional Management in Northern Benin. Hindawi Publishing Corporation. The Scientific World Journal, Volume 2013, Article ID 484536, 11 p. http://dx.doi.org/10.10.1155/2013/484536
Miège J. 1952. Contribution to the systematic study of West African Dioscorea. PhD thesis in Natural Science, Paris, p 226.
Sosnoskie LM, Webster TM, Dales D, Rains GC, Grey TL, Culpepper AS. 2009. ‘Pollen grain size, density, and settling velocity for palmer Amaranth (Amaranthus palmeri)’, Weed Science Society of America 57(4), p. 404–409. ISSN: 0043-1745 http://dx.doi.org/10.10.1614/WS-08-157.1/
Soulemane N. 2016. Exploration of the morphological and pollen variability of accessions of Amaranthus dubius (Amaranthaceae) of various origins. Master thesis in Genetics, Biotechnologies and Biological Resources, University of Abomey – Calavi p 57.
Yolou M, Ida Anizehou, Romaric Dossou-Yovo, Akpovi Akoegnonnou, Jean Didier Zongo, Jeanne Zoundjihèkpon, 2015 a. Inventory of sexual reproduction of African yams Dioscorea cayenensis – Dioscorea rotundata cultivated in Benin, International Journal of Biological and Chemical Sciences 9(2), p 737-750. http://dx.doi.org/10.4314/ijbcs.v9i2.14, http://indexmediccus.afro.who.int
Yolou M, Zoundjihèkpon J, Tiama D, Anizèhou SI, Assaba EI, Adéchokan HAAM, Zongo JD, Akoegninou A. 2015 b. ‘Evaluation of yam (Dioscorea cayenensis – Dioscorea rotundata) seed germination grown in Centre Benin’, International Journal of Advanced Research 3(12), p 277 – 284. ISSN: 2320-5407, http://dx.doi.org/10.10.21474/IJAR01/10116/, http://www.journalijar.com
Yolou M. 2016. Genetic diversity of cultivated African yams (Dioscorea cayenensis – D. rotundata complex and D. dumetorum) and the perception of peasants in Central Benin about intellectual property rights. PhD thesis of the University of Abomey-Calavi, Specialty Genetics and Plant Breeding, p 155.
Zoundjihèkpon J, Dansi A. 1998. Reproductive biology of African yams. In: The yam, age-old plant and future crop. Eds Berthaud J, Bricas N. et Marchand JL. CIRAD. 231-240 p.
Zoundjihékpon J, Hamon P, Hamon S, Tio-Toure B. 1995. Relationships between germination, flowering and level of sampling of yam seedlings from the Dioscorea cayenensis– rotundata complex. African Agronomy 7(3), p 223-235.
Zoundjihékpon J. 1993. Reproductive biology and genetics of cultivated yams of West Africa, Dioscorea cayenensis-rotundata, Doctoral thesis of the National University of Ivory Coast, Genetics and Plant Breeding, p 306.
Elie Idossou Assaba, Mounirou Yolou, Narcisse Denadi, Ida Sessi Anizehou, Jeanne Zoundjihekpon, 2020. Variability of pollen grains in clones of Dioscorea cayenensis – D. rotundata complex grown in centre of Benin. Int. J. Biosci., 16(2), 323-331.
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