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Various growth attributes of Escherichia coli cultures supplemented with Aloe vera as substrate

Research Paper | July 1, 2019

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Ikram-Ul Haq, Mariyam Shaikh, Munazza Raza Mirza¹, Asra Mahar, Mahnoor Dua, Komal Nazir

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Int. J. Biosci.15( 1), 532-541, July 2019

DOI: http://dx.doi.org/10.12692/ijb/15.1.532-541


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In present investigation, extracts of aloe vera plant organs incorporated into the TY-growth media and its effects on sub-merged Escherichia coli (E. coli) k1 fermentation are studied. The 18-hours cultures maintained with extracts of different aloe vera fresh organs (12.5%, v/v) in TY0 (1% Bacto-trypton, 0.5% NaCl, 0.5% yeast extract), TY1 (⅛ TY0), TY2 (TY1 + leaf-peel extract), TY3 (TY1 + root extract) and TY4 (TY1 + leaf-gel extract) medium. The cell multiplication observed high in leaf peel extract base TY2 cultures. Among the fermented biochemical analysis, maximum reducing sugars observed in TY4 than other medium (p ≥ 0.05), while flavonoids in TY3 medium non-signifiantly. The total phenolics noted higher in both TY3 and TY4 medium. Similarly hydrolytic enzymes have shown differential activities among the different cultures like as amylases activity in TY2 (gel), xylanases in TY4 (root) and lipases in TY3 (peel) medium measured significantly high. Overall, it is concluded that aloe vera is the best fermentation substrate for the production of various extra-cellular enzymes and essential substances. Even aloe vera is anti-bacterial agent, while its variant concentration in the fermentation medium has differential impacts on the propagation of micro-organisms.


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Various growth attributes of Escherichia coli cultures supplemented with Aloe vera as substrate

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