Welcome to International Network for Natural Sciences | INNSpub

Paper Details

Research Paper | October 15, 2022

VIEWS 31
| Download 10

Viability formulation with active Trichoderma asperellum TR3 in three packaging variation

Ratnawati Ratnawati, Arfan Arfan, Kasman Jaya, Sri Sudewi, Mr. Mufida

Key Words:


Int. J. Micro. Myco.15(4), 26-31, October 2022

Certification:

IJMM 2022 [Generate Certificate]

Abstract

Trichoderma asperellum TR3 has been applied as a biopesticide and Plant Growth Promotion Fungi (PGPF) but in the form of a suspension and substrate without the addition of carriers and adhesives so for long-term use and short shelf life. This study aims to obtain the right packaging formulation and have good viability, as well as a long storage time using talc and tapioca flour as the basic ingredients. The isolate collection was rejuvenated by growing in PDA medium, after completion of incubation for 48 hours, the growing fungal colonies were counted, isolated and purified, then made in the form of a powder starter. Test of various formulations of T. asperellum TR3 fungal powder packaging was carried out by viability test using a Completely Randomized Design (CRD), with packaging treatment on the formulation: P1 = Aluminum foil plastic, P2 = Bottle and P3 = Cetic plastic. Each treatment was repeated 3 times to obtain a combination of 9 treatment units. Furthermore, all packaging combinations were stored at room temperature. In the first month, 1 pack was taken for regrowth which aims to determine the viability of the conidia and then every two weeks for 12 weeks. The results showed that the average number of conidia colonies growing on various packages gave different viability results, but had no significant effect between formulations of various packages and viability. The average number of colonies that grew on aluminum foil plastic packaging was more in each observation of storage time compared to bottle and plastic packaging.

VIEWS 31

Copyright © 2022
By Authors and International Network for
Natural Sciences (INNSPUB)
http://innspub.net
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Viability formulation with active Trichoderma asperellum TR3 in three packaging variation

Agrios NA. 2005. Plant Pathology. Fifth Edition. Academic Press. San Diego, California.

Budiarti L, dan Nurhayati. 2014. Kelimpahan Cendawan Antagonis pada Rhizosfer Tanaman Kacang Panjang (Vigna sinensis L) Savi ex Hassk di Lahan Kering Indralayah Sumatera Selatan. Prosiding seminar Nasional Lahan Suboptimal Palembang. ISBN:979-587-529-9

Cook RJ, Dan Baker KF. 1983. The nature and practice of biological control of plant pathogens, APA Press the American Phytopathological Society. St. Paul, Minnesota.

Hanudin dan Budi Marwoto. 2012. Prospek Penggunaan Mikroba Antagonis Sebagai Agens Pengendali Hayati Penyakit Utama Pada Tanaman Hias Dan Sayuran, Jurnal Litban Pertanian Vol. 3(1)

Hayat RS, Ali U, Amara R, Khalid, Ahmed I. 2010. Soil beneficial bacteria and their role in Plan Growth Promotion: A Review Ann. Microbiol 60(4), 579-598.

Kusari S, Verma VC, Lamshoeft M, Spiteller M. 2012. An endophytic fungus from Azadirachta indica juss that produces azadirachtin. World J. Microbiol Biotechnol 28, 1287-1294.

Loves-Arevelo AG, Huerta-Palacios, Sanches-Vazquez JE. 1996. Comtamination Encountered uring Various Phase of Cultivation of Pleurotus ostreatus in Tropical Mexico. Mushroom Biology and Mushroom Product, Royse (De.) Penn State University P: 495-501

Ismail M, Ade Rosmana, Sylvia jam dan Ratnawati. 2020. shallot Basal Bulb Rot Management through Integration of Trichoderma asperellum, Composted Plant Residues and Natural Mulch. Journal Pure Appl Microbiol | 14(3), 1779-1788.

Nirwanto H, Dan Mujoka T. 2009. Eksplorasi dan kajian Keragaman Jamur Filoplen pada Tanaman Bawang Merah: Upaya Pengendalian Hayati Terhadap Penyakit Bercak Ungu (Alternaria porii). Bahan Seminar Nasional Pada Fak.Pertanian dan LPMM UPM Veteran Jawa Timur.

Novita E, Purbasari D, Mubarok MSM. 2021. Pendugaan Umur Simpan Bubuk Kopi Arabika Menggunakan Metode Arrhenius dengan Kemasan Aluminium Foil dan Plastik (Polipropilen). Jurnal Teknik Pertanian Lampung 10(3), 392-401.

Nur Amin, Muslim Salam, Asman, Ryan, Danial Rahim. 2015. Investigation of Endophytic Fungi towards Vascular Streick Dieback Oncobasidium theobromae on Seedling of Cocoa Plant. Journal of Applied Biological Sciences 9(2), 86-89.

Nur Amin, La Daha, Nurariaty Agus. 2017. Endophytic Fungi as Biopesticide Againts Rice Black Bug on Rice Plant. Research Journal of Pharmaceutical, Biological and Chemical Sciences 8(2).

Rashid MI, Mujawar LH, Shahzad T, Almeelbi T, Ismai IMI. 2016. Bacteria and fungi cams contribute to nutrients bioavailability and aggregate formation in degrade soils. Microbiol. Res.183, 26-41.

Ratna Sari Dewi, Giyanto, Meity Suradji Sinaga, Dadang dan Bambang Nuryanto. 2020. Bakteri Agens Hayati Potensial terhadap Patogen Penting pada Padi, Jurnal Fitofatologi 16(1), 37-48

Ratnawati, Sylvia Sjam, Ade Rosmana dan Untung Surapati Tresnaputra. 2019. Impact of Pesticides on the Diversity of Fungi at Local Shallot in Palu, Indonesia. Int. J. Curr. Microbiol. App. Sci. 8(8), 730-738

Ratnawati R, Sylvia Sjam, Ade Rosmana dan Untung Surapati Tresnaputra. 2020. Endophytic Trichoderma Species of Palu Valley Shallot Origin with Potential for Controlling Purple Blotch Pathogen Alternaria pori. International Journal of Agriculture and Biology 23(05), 977-982.

Ratnawati dan K Jaya. 2021. Seleksi Dan Identifikasi Cendawan Endofit Di Pertanaman Organik Bawang Merah Lokal Palu. Jurnal Agrotech 11(1), 13-19, Juni 2021

Rosmana A, Sylvia Sjam, Asman Asman, Nurul Jihad Jayanti, Satriana Satriana, Andi Tenri Padang, Andi Akbar Hakkar. 2018. Systemic Deployment of Trichoderma asperellum in Theobrama cacao Regulator Co-occuring Dominant Fungal Endophytes Colonization. Journal of Pure and Applied Microbiology 12(3), 1071-1084.

Sayang Y. 2009. Kajian Keragaman Mikoba Antagonis Rizosfer Dan Pemanfaatannya dalam Mengedalikan Penyebab Penyakit Busuk Umbi (Fusarium oxysporum Schecht) Pada Tanaman Bawang Merah. Jurnal Sains & Teknologi 9(3), 165-170.

Santoso SE, Soesanto L, dan Dwi Haryanto TA. 2007. Penekanan Hayati Penyakit Moler Pada Bawang Merah Dengan Trichoderma harzianum, Trichoderma koningii, dan Pseudomonas fluorescens P60. J. HPT Tropika 7(1), 53-61.

Siti Hadiyanti, Bonny Purnumo, Wahyu Soekarno dan Titek Siti Yuliani. 2017. Kemampuan Mikroba Endofit dan Risosfer Tanaman Karet dalam Mengendalikan Rigidoporus lignosus. Jurnal Fitopatologi Indonesia 13(5), 153-160.

Suherah, Tutik Kuswinanti, Ade Rosmana dan Burhanuddin Rasyid. 2018. The Effect Of Organic Medium Use In Formulation Of Trichoderma Harzianum and Pleurotus Ostreatus In Viability And Decomposition Of Cacao Pod Husks Waste. J. Biotechnol 15(1), 107-112.

SUBMIT MANUSCRIPT

Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background