Zinc oxide nanoparticles (ZnO NPs) induced nephrotoxicity in male sprague dawley rats

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Research Paper 01/11/2018
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Zinc oxide nanoparticles (ZnO NPs) induced nephrotoxicity in male sprague dawley rats

Sana Kausar, Farhat Jabeen, Salma Sultana, Azhar Rasul
Int. J. Biosci.13( 5), 457-463, November 2018.
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Abstract

Nano size particles (<100nm) have various applications in electronics, coating, cosmetics, packaging and biotechnology. Zinc Oxide nanoparticles (ZnO NPs) are being used in ceramics, leather manufacturing, plastics, rubber, glass, fire retardants and batteries along with having antimicrobial and anticancerous properties. In present research, 25 post weaning male Sprague Dawley rats of similar weight were procured from the animal house of Government College University Faisalabad after approval of the ethical committee on animal experimentation. Rats were kept in 5 cages (n=5) and varying levels of ZnO NPs were injected intraperitoneally (i.p.) for 28 days on alternate days to treated groups at the dose of either 10 or 20 or 30 mg/kg and named as group one (G1), two (G2) and three (G3), respectively for the assessment of toxicity for better understanding of precautionary measures in near future. Without any treatment groups i.e., control (C) and saline(S) received normal diet and saline water (0.9% sodium chloride), respectively. Histological changes were investigated in kidney tissues of all groups. Groups receiving 10 and 20mg/kg of NPs showed moderate pathological changes like atrophic glomerulus, inter-tubular space, degeneration of tubular epithelium and tubules and accumulation of ZnONPs. While, G3 group showed congestion, accumulation of RBCs and hemorrhages in kidney tissues along with above noticed variations. Whereas, no alterations were seen in control groups (C &S). It is concluded that ZnO NPs at higher concentration are more toxic to Sprague Dawley rats than at lower concentrations.

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