Antioxidant activity of essential oil from Lawsonia inermis Linn from Pakistan
Paper Details
Antioxidant activity of essential oil from Lawsonia inermis Linn from Pakistan
Abstract
The present study was designed to assess the constituents of essential oil of lawsonia innermis Linn by GC-MS and measure the antioxidant ability by using DPPH stable radical. Essential oil was extracted from the leaves of lawsonia Inermis Linn by hydro-distillation using modified Dean Stark Apparatus, followed by drying with anhydrous sodium sulphate. GC-MS studies were prformed for constituents determination by comparison the retention indices and mass spectra with those obtained the library on National Institute of Standard and Technology (NIST). Antioxidant activity of the essential oil of the lawsonia immerimis was performed by using 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay. Light yellow colored oil with 0.4% yield was obtained. The chemical constituents were analyzed by GC-MS, which revealed 21 compounds, out of which 13 were identified and eucalyptol (18.141%). α-pinene (13.345%) and linalool (10.527%) were major components. The essential oil was tested for DPPH scavenging assay and it was found concentration dependant and maximum antioxidant activity 88% was observed for sample at 100 μl concentration while BHT showed activity 92% for the similar concentration. The present study suggested that essential oil of lawsonia innermis is a good source of natural antioxidant.
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