Comparison of phenolic content and antioxidant activity of methanolic and ethanolic extracts of Limoniastrum guyonianum
Paper Details
Comparison of phenolic content and antioxidant activity of methanolic and ethanolic extracts of Limoniastrum guyonianum
Abstract
Limoniastrum guyonianum Durieu ex Boiss. (Plumbaginaceae) popularly known as ”Zeta” is mainly mentioned to cure constipation, diabetes, leprosy, hypertension and other ailments. Its efficacy is widely acclaimed among communities in Southeastern Algeria. The present study is designed to determine the phenolic composition and the antioxidant activities of hydro alcoholic extracts of this raw material. The phenolic extracts of the dried and powdered aerial parts of Limoniastrum guyonianum were obtained by conventional maceration using 70% methanol and ethanol in water as solvent. The hydro alcoholic extracts were evaluated for their phenolic contents via colorimetric methods and were investigated for their antioxidant capacities using phosphomolybdate, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) tests. The highest amounts of total phenolic (121.51 mg GAE/g DW), flavonoids (8.65 mg RE/g DW), condensed tannins (44.57 mg EPE/g DW) and overall antioxidant activity (55.57 mg AAE/g DW) were found in the methanolic extracts. The IC50 values of methanolic and ethanolic extracts for DPPH radical scavenging activity were 31.10 and 23.25 µg/ml, respectively. As for ABTS (successively, 22.71 and 21.25 µg/ml), the top 26.97 and 08.60 % scavenging activity were observed in plant extracts. The trend observed for bioactive compounds was confirmed by antioxidant activity assays and high correlation values were obtained with all studied antioxidant compounds. Collectively, the findings suggested that Limoniastrum guyonianum is a natural source of antioxidant substances, which may be useful for curing diseases arising from oxidative deterioration.
Azlim Almey AA, Ahmed Jalal Khan C, Syed Zahir I, Mustapha Suleiman K, Aisyah MR, Kamarul Rahim K. 2010. Total phenolic content and primary antioxidant activity of methanolic and ethanolic extracts of aromatic plants leaves. International Food Research Journal 17, 1077-1084.
Brand-Williams W, Cuvelier ME, Berset C. 1995. Use of a free radical method to evaluate antioxidant activity. LWT- Food Science and Technology 28, 25-30. http://dx.doi.org/10.1016/S0023-6438(95)80008-5
Canadanovic-Brunet JM, Djilas SM, Cetkovic GS, Tumbas VT, Mandic AI, Canadanovic VM. 2006. Antioxidant activities of different Teucrium montanum L. extracts. International Journal of Food Science and Technology 41, 667–673. http://dx.doi.org/10.1111/j.1365-2621.2006.01133.x
Chaabi M, Beghidja N, Benayache S, Lobstein A. 2008. Activity-guided isolation of antioxidant principles from Limoniastrum feei (Girard) Blatt. Z. Naturforsch. 63c, 801-807.
Chahma A, Djebar MR. 2008. Les espèces médicinales spontanées du Sahara septentrional algérien : distribution spatio-temporelle et étude ethnobotanique. Revue Synthèse 17, 36-45.
Cui K, Luo X, Xu K, Ven Murthy MR. 2004. Role of oxidative stress in neurodegeneration: recent developments in assay methods for oxidative stress and nutraceutical antioxidants. Prog Neuropsychopharmacol Biol Psychiatry 28, 771-799. http://dx.doi.org/10.1016/j.pnpbp.2004.05.023
Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocker P, Vidal N. 2006. Antioxidant activity of some algerian medicinal plants extracts containing phenolic compounds. Food Chemistry 97, 654-660. http://dx.doi.org/10.1016/j.foodchem.2005.04.028.
El-Hela A, Abudullah A. 2010. Antioxidant and antimicrobial activities of methanol extracts of some Verbena species: In vitro evaluation of antioxidant and antimicrobial activity in relation to polyphenolic content. Journal of Applied Sciences Research 6, 683-689.
Gallardo C, Jimenez L, Garcia-Conesa MT. 2006. Hydroxycinnamic acid composition and in vitro antioxidant activity of selected grain fractions. Food Chemistry 99, 455-463. http://dx.doi.org/10.1016/j.foodchem.2005.07.053
Hadjadj S, Bayoussef Z, Ould El Hadj-Khelil A, Beggat H, Bouhafs Z, Boukaka Y, Khaldi IA, Mimouni S, Sayah F, Tey M. 2015. Ethnobotanical study and phytochemical screening of six medicinal plants used in traditional medicine in the Northeastern Sahara of Algeria (area of Ouargla). Journal of Medicinal Plants Research 8, 1049-1059. http://dx.doi.org/10.5897/JMPR2015.5932.
Hamdoon AM, Salmin KA, Awad GA. 2013. Antioxidant and quantitative estimation of phenolic and flavonoids of three halophytic plants growing in Libya. Journal of Pharmacognosy and Phytochemistry 2, 89-94.
Hseu YC, Chang WH, Chen CS, Liao JW, Huang CJ, Lu FJ, Chia YC, Hsu HK, Wu JJ, Yang HL. 2008. Antioxidant activities of Toona sinensis leaves extracts using different antioxidant models. Food and Chemical Toxicology 46, 105-114. http://dx.doi.org/10.1016/j.fct.2007.07.00.3
Iloki–Assanga SB, Lewis–Lujan LM, Lara–Espinoza CL, Gil Salido AA, Fernandez–Angulo D, Rubio Pino JL, Haines DD. 2015. Solvent effects on phytochemical constituent profiles and antioxidant activities, using four different extraction formulations for analysis of Bucida buceras L. and Phoradendron californicum. BMC Research Notes 8, 1-14. http://dx.doi.org/10.1186/s13104-015-1388-1.
Li HB, Wong CC, Cheng KW, Chen F. 2008. Antioxidant properties in vitro and total phenolic contents in methanol extracts from medicinal plants. LWT 41, 385–390. http://dx.doi.org/10.1016/j.lwt.2007.03.011
Lister E, Wilson P. 2001. Measurement of total phenolics and ABTS assay for antioxidant activity (personal communication). Crop Research Institute: Lincoln, New Zealand.
Maisuthisakul P, Suttajit M, Pongsawatmanit R. 2007. Assessment of phenolic content and free radical-scavenging capacity of some Thai indigenous plants. Food Chemistry 100, 1409-1418. http://dx.doi.org/10.1016/j.foodchem.2005.11.032
Naqvi SAR, Ain QU, Khan ZA, Hussain Z, Shahzad SA, Yar M, Ghaffar A, Mahmood N, Kousar S. 2013. Antioxidant, antibacterial and antiproliferative activities of areal parts of Swertia chirata (Bush Ham) plant extracts using in vitro models. Asian Journal of Chemistry 25, 5448-5452. http://dx.doi.org/10.14233/ajchem.2013.14700.
Ozenda P. 2004. Flore et végétation du Sahara. CNRS Editions, Paris, 566.
Peschel W, Sanchez-Rabaneda F, Diekmann W, Plescher A, Gartzia I, Jimenez D, Lamuela- Raventos R, Buxaderas S, Codina C. 2006. An indusrial approach in the search of natural antioxidants from vegetable and fruit wastes. Food Chemistry 97, 137-150. http://dx.doi.org/10.1016/j.foodchem.2005.03.033
Porter LJ, Hrstich LN, Chan BG. 1985. The conversion of procyanidins and prodelphinidins to cyanidin and delphinidin. Phytochemistry 25, 223-230. http://dx.doi.org/10.1016/S0031-9422(00)94533-3.
Prieto P, Pineda M, Aguilar M. 1999. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical Biochemistry 269, 337-341. http://dx.doi.org/10.1006/abio.1999.4019.
Quettier-Deleu C, Gressier B,Vasseur J, Dine T, Brunet C, Luyckx M, Cazin M, Cazin JC, Bailleul F, Trotin F. 2000. Phenolic compounds and antioxidant activities of buckweat (Fagopyrum esculentum Moench) hulls and flour. Journal of Ethnopharmacology 72, 35-42. http://dx.doi.org/10.1016/S0378-8741(00)001963
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine 26, 1231-1237. http://dx.doi.org/10.1016/S0891-5849(98)003153
Rice-Evans CA, Miller NJ, Bolwell PG, Bramley PM, Pridham JB. 1995. The relative antioxidant activities of plant derived polyphenolic flavonoids. Free Radic Res 22, 375-383. http://dx.doi.org/10.3109/1071576950914564.9
Roginsky V, Lissi EA. 2005. Review of methods to determine chain-breaking antioxidant activity in food. Food Chemistry 92, 235-254. http://dx.doi.org/10.1016/j.foodchem.2004.08.004
Rubalya Valantina S, Neelamegam P. 2015. Selective ABTS and DPPH- radical scavenging activity of peroxide from vegetable oils. International Food Research Journal 22, 289-294.
Samaniego Sanchez C, Troncoso Gonzalez AM, García- Parrilla MC, Quesada Granados JJ, López Garcia de la Serrana H, López Martinez MC. 2007. Different radical scavenging tests in virgin olive oil and their relation to the total phenol content. Analytica Chimica Acta 593, 103–107. http://dx.doi.org/10.1016/j.aca.2007.04.037
Sakihama Y, Cohen MF, Grace SC, Yamasaki H. 2002. Plant phenolic antioxidant and prooxidant activities: phenolics-induced oxidative damage mediated by metals in plants. Toxicology 177, 67–80. http://dx.doi.org/10.1016/S0300-483X(02)001968
Sarwar S, Anwar F, Raziq S, Nadeem M, Zreen Z, Cecil F. 2012. Antioxidant characteristics of different solvent extracts from almond (Prunus dulcis L.) shell. Journal of Medicinal Plants Research 6, 3311-3316. http://dx.doi.org/10.5897/JMPR11.1723
Telli A, Esnault MA, Ould El Hadj Khelil A. 2016. An ethnopharmacological survey of plants used in traditional diabetes treatment in south-eastern Algeria (Ouargla province). Journal of Arid Environments 127, 82-92. http://dx.doi.org/10.1016/j.jaridenv.2015.11.005
Trabelsi N, Megdiche W, Ksouri R, Falleh H, Oueslati S, Soumaya B, Hajlaoui H, Abdelly C. 2010. Solvent effects on phenolic contents and biological activities of the halophyte Limoniastrum monopetalum leaves. LWT – Food Science and Technology 43, 632-639. http://dx.doi.org/10.1016/j.lwt.2009.11.003
Trabelsi N, Waffo-Téguo P, Snoussi M, Ksouri R, Mérillon JM, Smaoui A, Abdelly C. 2013. Variability of phenolic composition and biological activities of two Tunisian halophyte species from contrasted regions. Acta Physiol Plant, 1-13. http://dx.doi.org/10.1007/s11738-012-1115-7.
Von Gadov A, Joubert E, Hansmann CF. 1997. Comparison of the antioxidant activity of aspalathin with that of other plant phenols of Roobies tea (Aspalathus linearis), α-tocopherol, BHT, and BHA. Agricultural and Food Chemistry 45, 632-638. http://dx.doi.org/10.1021/jf960281n.
Wong CC, Li HB, Cheng KW, Chen F. 2006. A systematic survey of antioxidant activity of 30 Chinese medicinal plants using the ferric reducing antioxidant power assay. Food Chemistry 97, 705-711. http://dx.doi.org/10.1016/j.foodchem.2005.05.049.
Yokozawa T, Chen CP, Dong E, Tanaka T, Nonaka GI, Nishioka I. 1998. Study on the inhibitory effect of tannins and flavonoids against 1,1-diphenyl-2-picrylhydrazyl radical. Biochemical Pharmacollogy 56, 213-222. http://dx.doi.org/10.1016/S0006-2952(98)001282
Soumia Hadjadj, Akila Khadir, Sabrina Bensaci, Mohamed Didi Ould El Hadj, Aminata Ould El Hadj-Khelil (2016), Comparison of phenolic content and antioxidant activity of methanolic and ethanolic extracts of Limoniastrum guyonianum; IJB, V9, N6, December, P35-44
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