Mineral and fat analysis of Theobroma cacao nibs incorporated in locally produced Banana bread

Paper Details

Research Paper 15/10/2022
Views (1015)
current_issue_feature_image
publication_file

Mineral and fat analysis of Theobroma cacao nibs incorporated in locally produced Banana bread

Ronel T. Pacubat
J. Biodiv. & Environ. Sci. 21(4), 81-94, October 2022.
Copyright Statement: Copyright 2022; The Author(s).
License: CC BY-NC 4.0

Abstract

The current study aims to determine the elemental and lipid components of cocoa nibs added in locally-produced banana bread through established methods in food chemistry. X-ray Fluorescence (XRF) Spectroscopy revealed that significant amount of Potassium (40.83%), Calcium (33.11%), Iron (33.46%), Phosphorous (6.17%), Silicon (4.55%), Sulfur (1.80%) is present while Copper (0.32%), Cadmium (0.49%), Manganese (0.84%), Titanium (0.72%), Nickel (0.43%), and Zinc (0.28%) are in trace amounts from the fifty grams (50 g) of cocoa nibs. Aside from traces of Cadmium, no other heavy metals were detected. Cocoa nibs incorporated in locally produced banana bread resulted in addition of Iron (2.88mg), Potassium (179.86mg), Zinc (0.79mg), Calcium (33.20mg) per 100 grams of the banana bread as revealed by inductive coupled plasma-optical emission spectroscopy (ICP-OES) and atomic absorption spectroscopy (AAS). Furthermore, lipid components of the banana bread with cocoa nibs include 19.64% crude fat comprising 13.08% saturated fat, 0% transaturated fat, 29.70mg cholesterol, 1,599 international units (IU) or 0.95mg of vitamin A using AOAC official methods, gas chromatography (GC) and high performance liquid chromatography (HPLC). Hence, Cocoa Nibs is not just a good aroma and flavoring enhancer but also a nutritional enhancer for the locally-produced banana bread.

Abbaspour N, Hurrell R, Kelishadi R. 2014. Review on iron and its importance for human health. Journal of research in medical sciences: The official Journal of Isfahan University of Medical Sciences 19, 164-174.

Akinyele IO, Shokunbi OS. 2015. Comparative analysis of dry and wet digestion methods for the determination of trace and heavy metals in food. Food Chemistry 173, 682-684.

Bakkali K, Martos NR, Souhail B, Ballesteros E. 2009. Characterization of trace metals in vegetables by graphite furnace atomic absorption spectrometry after closed vessel microwave digestion. Food Chem 116, 590-594.

Bhowmik K. Sampath Kumar P. 2010. A potential medicinal importance of zinc in human health and chronic disease. Department of Pharmaceutical Sciences, Coimbatore Medical College, Coimbatore, Tamilnadu, India.

Chen P, Bornhorst J, Aschner M. 2018. Manganese metabolism in humans. Front Biosci (Landmark Ed) 23, 1655-1679.

Fontana Jr AJ, Campbell CS. 2004. Water activity (Chapter 3 In: Nollet L (ed.) Handbook of Food Analysis, 2nd edn. Marcel Dekker, Inc., New York p. 39-54.

Food and Nutrition Research Institute (FNRI). 2002. Recommended energy and nutrient intake and List of PDRI.

Hewlings, Susan and Douglas Kalman. 2019. Sulfur in Human Health. Department of Nutrition, Central Michigan University USA.

Iqbal MP. Trans fatty acids- A risk factor for cardiovascular disease. Pak J Med Sci 30, 194-197.

Katz DL, Doughty K, Ali A. 2011. Cocoa and chocolate in human health and disease. Antioxidants & redox signaling 15, 2779-2811.

Kuboyama K, Sasaki N, Nakagome Y, Kataoka M. 2005. Wet Digestion. Anal Chem 360, 184-191.

Nielsen, Forest. 2014. Update on the possible nutritional importance of silicon. Journal of Trace Elements in Medicine and Biology 28.

Oliva, Manuel. Karol Rubio. Melissa Epquin. Gladys Marlo and Santos Leiv. 2017. Cadmium Uptake in Native Cacao Trees in Agricultural Lands of Bagua, Peru.

Piste Pravina, Didwagh Sayaji and Mokashi Avinash. 2012. Calcium and its Role in Human Body P.G. Department of Chemistry, Y. C. Institute of Science, Satara, Maharashtra, India.

Takeda E, Yamamoto H, Nashiki K, Sato T, Arai H, Taketani Y. 2004. Inorganic phosphate homeostasis and the role of dietary phosphorus. J Cell Mol Med 8, 191-200.

Tibau, Anita V. Blanche D. Grube. Braulio J. Velez. Victor M. Vega. Joachim Mutter. 2017. Titanium exposure and human health. Center for Environmental and Toxicological Research, University of Puerto Rico, Newport Beach CA.

Related Articles

Protective effect of polyherbal formula nalpamaram on the ethanol-induced toxicity in Hep G2 cell lines

S. Jyothilekshmi, Jini Joseph, J. Biodiv. & Environ. Sci. 27(5), 137-142, November 2025.

Household socio-agricultural profiles and the adoption of crop protection strategies in human-wildlife conflict contexts: Insights from western Côte d’Ivoire around mount Sangbé National Park

Koffi Kouamé Christophe, Ouffoue Affoué Eugénie Naomie, Gagbé Dalié Sylvestre, Beda Alex, J. Biodiv. & Environ. Sci. 27(5), 91-103, November 2025.

Influence of biosynthesized silver nanoparticles on pollen germination and tube growth in Catharanthus roseus (L.) G. Don

Abhijit Limaye, Shreya Mulay, Jidnyasa Jangale, Rasadnya Joshi, Swapna Sathe, Kishor Bhosale, J. Biodiv. & Environ. Sci. 27(5), 85-90, November 2025.

Genetic diversity of parasitoids and entomopathogenic nematodes of Spodoptera frugiperda Smith, 1797 (Lepidoptera: Noctuidae) in Senegal

Farma Fall Babou, Toffène Diome, Mama Racky Ndiaye, Mbacké Sembene, J. Biodiv. & Environ. Sci. 27(5), 69-84, November 2025.

Environmental and socio-economic impacts of pollution by Eichhornia crassipes (Mart.) Solms in the waters of Dams No. 2 and No. 3 in the city of Ouagadougou, Burkina Faso

Florent Y. Lankoande, Jerome T. Yameogo, Asseta Tabsoba, S. E. I. Bama, J. Biodiv. & Environ. Sci. 27(5), 59-68, November 2025.

Evaluation of grains and haulms production of soybean varieties in production areas with high livestock potentiality in Benin

Assouan Gabriel Bonou, Alain Sèakpo Yaoitcha, Serge Aklinon, J. Biodiv. & Environ. Sci. 27(5), 51-58, November 2025.