Evaluation of the quality of SARS COV 2 positive nasopharyngeal samples stored in liquid nitrogen at the biobank (Pasteur institute of Côte d’Ivoire)

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Research Paper 04/01/2024
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Evaluation of the quality of SARS COV 2 positive nasopharyngeal samples stored in liquid nitrogen at the biobank (Pasteur institute of Côte d’Ivoire)

Julie José Rita Bouagnon, Marcelle Money, Kouao Maxime Diané, Bahou Roger Dehé, Souleymane Cissé, Kouadio Ayébé Edwige Aka, Kouamé Ambroise Kintossou, Litio Sinali Coulibaly, Hervé Alberic Kadjo, Jean David N’Guessan, Alico Joseph Djaman, Mireille Dosso
Int. J. Biosci. 24(1), 61-65, January 2024.
Copyright Statement: Copyright 2024; The Author(s).
License: CC BY-NC 4.0

Abstract

The SARS Cov2 positive samples from the COVID 19 pandemic surveillance were subjected to quality control after storage at the biobank of the Pasteur Institute of Côte d’Ivoire. They were evaluated one year after their cryopreservation by a PCR test (polymerase chain reaction) and the threshold values of the cycle were recorded. PCR test results indicated 100% concordance of all nasopharyngeal specimens that tested positive for SARS CoV2. It appears that nasopharyngeal samples packaged in high security straws and stored at -196°C are still viable for detecting SARS-CoV-2 after one year of storage. A reproducibility of the preservation protocol can be carried out to standardize the preservation technique. An annual program for evaluating the conservation of samples can be instituted at the biobank as a form of quality control.

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