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Estimation of various properties of bioactive compounds isolated from Catharanthus roseus leaf

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Research Paper 09/12/2024
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Estimation of various properties of bioactive compounds isolated from Catharanthus roseus leaf

Shahin Aziz, Kousik Saha
Int. J. Biosci.25( 6), 330-337, December 2024.
Certificate: IJB 2024 [Generate Certificate]
Key: Antibacterial activityCatharanthus roseusCytotoxicityFree radical scavenging activity

Abstract

Lupeol, stigmasterol, ursolic acid, myricetin, and naringenin were isolated from n-hexane, ethyl acetate, and methanolic extracts of Catharanthus roseus leaves to assess their free radical scavenging, cytotoxic, and antibacterial activities. The determination of the free radical scavenging activity (FRSA) of the isolated compounds was performed by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) method along with UV-Visible spectroscopy. The brine shrimp lethality bioassay (BSLT) method was applied to determine cytotoxic activity. For antibacterial screening, the disk diffusion method was employed, with streptomycin (10 μg/disc) serving as a control. Among the compounds, myricetin exhibited noteworthy free radical scavenging activity with an IC50 6.18 µg/mL. Ursolic acid had a strong cytotoxic effect in the brine shrimp lethality bioassay, with an LC50 of 0.72. Naringenin outperformed streptomycin in terms of antibacterial activity against the pathogenic microorganisms that have been tested. The FRSA, cytotoxicity, and antibacterial activity of isolated compounds were consistent with the traditional uses of this plant.

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