Rhizospheric fungal diversity in the medicinal plants of Rewa District, Madhya Pradesh, India
Paper Details
Rhizospheric fungal diversity in the medicinal plants of Rewa District, Madhya Pradesh, India
Abstract
Rhizospheric fungi play pivotal roles for both soil and plant health. These fungi improving the growth of the plants and enhance the production of secondary metabolites. In the rhizosphere of medicinal plants, a very complex and diverse microbial community is present, including fungi, that significantly influences plant health and secondary metabolite production. Rhizospheric fungi are attracting researchers towards medicinal plants uses due to the presence of bioactive compounds, which consist of alkaloids, peptides, steroids, phenols, quinones, flavonoids, terpenoids, etc. The present study has been undertaken to isolate and identify the soil fungi associated with the rhizosphere of some medicinal plants, viz., Ocimum sanctum, Asparagus officinalis, Cymbopogon citratus, and Mentha arvensis of the Rewa district of MP, India. Total eight fungal species were isolated from the rhizosphere of selected medicinal plants. The highest number of fungal colonies was found in the rhizosphere of Cymbopogon citratus. Maximum fungal diversity was in the rhizosphere of Ocimum sanctum, with six species while minimum was in Asparagus officinalis with three species. Aspergillus fumigatus, Aspergillus niger, and Penicillium sp. were more frequent, which were associated with all these plants except Cymbopogon citratus. These findings suggested that medicinal plants are associated with different rhizosphere fungi, which are helpful for developing sustainable agriculture practices.
Ahmad N, Bhat MY, Wani AH, Peer LA. 2021. Rhizosphere mycobiome diversity of medicinal plants: A review. Journal of Plant Sciences Research 37(1), 109–121.
Al-Abbasi SHA, Al-Majmaei AAM, Al-Naqib ATH, Hameed AM, Al-Samarraie MQ, Altaef AH. 2021. Isolation and identification of some fungi from rhizospheric soils of some wild plants at Samarra University, Iraq. Caspian Journal of Environmental Sciences 19(5), 829–839. https://doi.org/10.22124/CJES.2021.5232
Badri DV, Vivanco JM. 2009. Regulation and function of root exudates. Plant, Cell & Environment 32(6), 666–681. https://doi.org/10.1111/j.1365-3040.2009.01926.x
Bais HP, Weir TL, Perry LG, Gilroy S, Vivanco JM. 2006. The role of root exudates in rhizosphere interactions with plants and other organisms. Annual Review of Plant Biology 57, 233–266. https://doi.org/10.1146/annurev.arplant.57.032905.105159
Caruso G, Abdelhamid MT, Kalisz A, Sekara A. 2020. Linking endophytic fungi to medicinal plants therapeutic activity: A case study of Asteraceae. Agriculture 10(7), 286. https://doi.org/10.3390/agriculture10070286
Dakora FD, Phillips DA. 2002. Root exudates as mediators of mineral acquisition in low-nutrient environments. Plant and Soil 245(1), 35–47. https://doi.org/10.1023/A:1020809400075
Dubey S, Tripathi T, Tripathi S, Kumar V, Hasan S, Khan S, Singh AP. 2026. Responsiveness of arbuscular mycorrhizal symbiosis in plants during abiotic stress. International Journal of Microbiology and Mycology 22(2), 1–20. https://doi.org/10.12692/ijmm/22.2.1-20
Gaddeyya G, Niharika PS, Bharathi P, Ratna Kumar PK. 2012. Isolation and identification of soil mycoflora in different crop fields at Salur Mandal. Advances in Applied Science Research 3(4), 2020–2026. https://www.primescholars.com/articles/isolation-and-identification-of-soil-mycoflora-in-different-crop-fieldsat-salur-mandal.pdf
Galovičová L, Borotová P, Valková V, Vukic MD, Terentjeva M, Kačániová M. 2023. Antifungal efficacy of mint essential oil against Penicillium sp. inoculated on carrots. Journal of Microbiology and Infectious Diseases 13(1). https://dergipark.org.tr/en/download/article-file/2781340
Gillman JC. 1956. Manual of Soil Fungi. 2nd ed. Ames, Iowa: Iowa State College Press.
Jena SK, Tayung K, Rath CC, Parida D. 2015. Occurrence of culturable soil fungi in a tropical moist deciduous forest, Similipal Biosphere Reserve, India. Journal of Microbiology 46(1), 85–96.
Jwieli LA, Elramli NA, Othman SR, Matuoog N. 2021. Isolation and identification of soil fungi from Ghemins, Libya. International Journal of Science and Research 10(2), 667–673. https://doi.org/10.21275/SR21209231652
Khan S, Kharwar PK, Singh V, Begum M, Kushwaha K, Singh E, Mishra C, Maurya P, Singh S, Anubha, Pathak S, Singh AP. 2024. A comprehensive review on arbuscular mycorrhizal fungi: Impact on plant growth and nutrient acquisition. Research Communication 2(2), 56–64.
Khan S, Kushwaha K, Kharwar PK, Singh AP. 2025. Arbuscular mycorrhizal fungi: An environment-friendly approach for sustainable agriculture. Agri Research & Technology: Open Access Journal 29(1), 556434. https://doi.org/10.19080/ARTOAJ.2025.29.556434
Khan S, Singh AP. 2024. Microbial inoculants: Effective biofertilizers for improving crop productivity. International Journal of Environmental Sciences & Natural Resources 34(3), 556390. https://doi.org/10.19080/IJESNR.2024.34.556390
Khan S, Singh AP. 2026. Beneficial soil microorganisms: Role in plant growth and soil fertility: A review. Indian Journal of Agricultural Research. https://doi.org/10.18805/IJARe.A-6440
Khan SA, Hamayun M, Yoon H, Kim HY, Suh SJ, Hwang SK, Bae IJ, Kim KY, Lee IJ, Choo YS, Yoon UH, Kong WS, Lee BM, Kim JG. 2008. Plant growth promotion and Penicillium citrinum. BMC Microbiology 8, 231. https://doi.org/10.1186/1471-2180-8-231
Khuseib HF, Al-Sadi AM, Al-Riyamy BZ, Maharachchikumbura SSN, Khalfan Al-Ruqaishi H, Velazhahan R. 2020. Alternaria alternata and Neocosmospora sp. from the medicinal plant Euphorbia larica exhibit antagonistic activity against Fusarium sp., a plant pathogenic fungus. All Life 13(1), 223–232. https://doi.org/10.1080/26895293.2020.1748540
Li X, De Boer W, Zhang Y, Ding C, Zhang T, Wang X. 2018. Suppression of soil-borne Fusarium pathogens of peanut by intercropping with the medicinal herb Atractylodes lancea. Soil Biology and Biochemistry 116, 120–130. https://doi.org/10.1016/j.soilbio.2017.10.016
Lynch JM. 1990. Introduction: Some consequences of microbial rhizosphere competence for plant and soil. In: Lynch JM (ed.). The Rhizosphere. New York: John Wiley & Sons. pp. 1–10.
Mir MA, Sawhney SS, Bhat MY, Jan H. 2017. Studies on the rhizospheric soil fungi associated with Nepeta cataria L. and Rumex dentatus L. in District Kulgam of Kashmir Valley. Indo American Journal of Pharmaceutical Research 7, 8756–8763.
Morgan JAW, Bending GD, White PJ. 2005. Biological costs and benefits to plant–microbe interactions in the rhizosphere. Journal of Experimental Botany 56, 1729–1739.
Nguyen NH, Song Z, Bates ST, Branco S, Tedersoo L, Menke J, Schilling JS, Kennedy PG. 2016. FUNGuild: An open annotation tool for parsing fungal community datasets by ecological guild. Fungal Ecology 20, 241–248. https://doi.org/10.1016/j.funeco.2015.06.006
Ortíz-Castro R, Contreras-Cornejo HA, Macías-Rodríguez L, López-Bucio J. 2009. The role of microbial signals in plant growth and development. Plant Signaling & Behavior 4(8), 701–712. https://doi.org/10.4161/psb.4.8.9047
Shah KK, Singh A, Basnet A, Pant B, Shrestha R, Khanal S, Lamichhane J, Joshi N, Pandey S. 2021. Role of soil microbes in sustainable crop production and soil health: A review. Agricultural Science and Technology 13(2), 83–92. https://doi.org/10.15547/ast.2021.02.019
Timonin MI. 1940. The interaction of higher plants and microorganisms. I. Microbial population of the rhizosphere of seedlings of certain cultivated plants. Canadian Journal of Research 18, 307–317. https://doi.org/10.1139/cjr40c-045
Vasudha S, Shivesh S, Prasad SK. 2013. Harnessing PGPR from rhizosphere of prevalent medicinal plants in tribal areas of Central India. Research Journal of Biotechnology 8(6), 76–85.
Walker TS, Bais HP, Grotewold E, Vivanco JM. 2003. Root exudation and rhizosphere biology. Plant Physiology 132(1), 44–51. https://doi.org/10.1104/pp.102.019661
Vasundhara Singh, Pawan Kumar Kharwar, Salman Khan, Avinash Pratap Singh*, 2026. Rhizospheric fungal diversity in the medicinal plants of Rewa District, Madhya Pradesh, India. Int. J. Microbiol. Mycol., 22(3), 1-8.
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