Polyphenolic content and antioxidant activity of hydroalcohlic and alcoholic extract of Thymus vulgaris

Paper Details

Research Paper 01/05/2013
Views (673)
current_issue_feature_image
publication_file

Polyphenolic content and antioxidant activity of hydroalcohlic and alcoholic extract of Thymus vulgaris

Anoosh Eghdami, Mojtaba Eizadi, Fatemeh Sadeghi
J. Biodiv. & Environ. Sci. 3(5), 94-101, May 2013.
Copyright Statement: Copyright 2013; The Author(s).
License: CC BY-NC 4.0

Abstract

Thymus vulgaris (thyme) (like some other wild Thymus species (wild thyme)) possesses a wide range of biological activities including expectorant, spasmolitic, sedative, antibacterial, antifungal, antioxidant. The objective of this paper was a screening of flavonoid content in Thymus vulgaris, the methanol extract of plant material was used for High-performance liquid chromatography (HPLC) analysis. Additionally, the antioxidant activities were also determined by ferric reducing antioxidant power (FRAP), 1, 1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity and reducing power methods. Hydroalcoholic extract had the higher flavonoid and total phenolic contents than alcoholic extract of Thymus vulgaris .A strong positive correlation of R2 = 0.92 between total phenolic content and antioxidant activity was observed in this investigation. This study indicated that Thymus vulgaris exhibited the high antioxidant activity, flavonoid and phenolic content and can be used potentially as a readily accessible source of natural antioxidant.Antioxidant activities of polyphenols have been suggested to exert beneficial pharmacological effects on some diseases.

Benzie IFF, Strain JJ. 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anals Biochemical 239, 70– 76.

Bragaa PC, Dal Sasso M, Culici M, Bianchi T, Bordoni L, Marabini L. 2006. Anti-inflammatory activity of thymol: Inhibitory effect on the release of human neutrophil elastase. Pharmacology 77(3), 130-136.

Bragab PC, Dal Sasso M, Culici M, Galastri L, Marceca MT, Guffanti EE . 2006. Antioxidant potential of thymol determined by chemiluminescence inhibition in human neutrophils and cell-free systems. Pharmacology 76(2), 61-68.

Canadanovic-Brunet JM, Djilas SM, Cetkovic GS. 2005. Free-radical scavenging activity of wormwood (Artemisia absinthium) extracts. Journal of the Science of Food and Agriculture 85, 265-272.

Chen ZY, Chan PT. 1996. Antioxidative activity of green tea catechins in canola oil. Chemistry and Physics of Lipids 82, 163–172.

Isabelle D. Recovery ,separation and characterization of phenolic compounds and flavonoids from Maple produces. Oepartment of Food Science and Agricultural Chemistry. A tbesis submitted to the Faculty of Graduate Studies and Research in partial fulfillment ofthe requirement of the degree of Master of Science. 2000.

Kadifkova P, Gj. Stefkov, S. Kulevanova. 2000. Proceedings of the First Conference on Medicinal and Aromatic Plants of Southeast European COlli1lries, Arangelovac, FR Yugoslavia 11, 527-531.

Mensure Q, Sezen T. 1998. Drug Yield and Essential Oil of Thymus vulgaris L. as in Influenced by Ecological and Ontogenetical Variation. Turkish Journal of Agriculture and Forestry 22, 537-542

Nasapon P , Ampai P, Maltree S, Chaiyavat C, Pimporn L. 2010. Phenolic content and in vitro inhibitory effects on oxidation and protein glycation of some thahi medicinal plants. Pakistan Journal of Pharmaceutical Sciences 23(4), 403-408.

Negi PS, Chauhan AS, Sadia GA, Rohinishree YS, Ramteke RS. 2005. Antioxidant and antibacterial activities of various seabuckthorn (Hippophae rhamnoides L.) seed extracts. Food Chemistry 92, 119-124.

Ordon Ez AAL, Gomez JD, Vattuone MA, Isla MI. 2006. Antioxidant activities of Sechium edule (Jacq) Swart extracts. Food Chemistry 97, 452–458.

Shan B, Cai YZ, Sun M, Corke H . 2005. Antioxidant Capacity of 26 Spice Extracts and Characterization of Their Phenolic Constituents. Journal of Agricultural and Food Chemistry 53(20), 7749-7759.

SvetIana K, Tatjana KP. 2001. Antioxidant activity of essential oils of different wild Thymus vulgaris. Bulletin of the Chemists and Technologists of Macedonia 20(1), 61-66.

Tipu  MA,  Akhtar  MA,  Anjum  M,  Raja  MJ. 2006. New dime OF Mention dicinal plants as animal feed. Pakistan Veterinary Journal. J 26(3), 144-148.

Vioux-Chagnoleau C, Lejeune F, Sok J, Pierrard C, 2006. Marionnet C and Bernerd F. Reconstructed human skin: from photodamage to sunscreen photoprotection and anti-aging molecules. Journal of Dermatological Science 2(1), 1-12.

Wolfe K, Wu X, Liu RH. 2003. Antioxidant activity of apple peels. Journal of Agriculture and Food Chemistry 51, 609–614.

Yamamoto J, Yamada K, Naemura A, Yamashita T, Arai R. 2005. Testing various herbs for antithrombotic effect. Nutrition 21(5), 580-587.

Yen GH, Chen HY. 1996. Antioxidant activity of various tea extracts in relation to their antimutagenicity. Journal of Agricultural and Food Chemistry 43, 27–32.

Yuan  YV,  Bone  DE,  Carrington  MF.  2005. Antioxidant  activity  of  dulse  (Palmira  palmata) extract evaluated in vitro. Food Chemistry 91, 485-494.

Zheng W, Wang SY. 2001.  Antioxidant  Activity and Phenolic Compounds in Selected Herbs. Journal of Agricultural and Food Chemistry  49(11), 5165-5170.

Related Articles

Agroforestry in woody-encroached Sub-Saharan savannas: Transforming ecological challenges into sustainable opportunities

Yao Anicet Gervais Kouamé, Pabo Quévin Oula, Kouamé Fulgence Koffi, Ollo Sib, Adama Bakayoko, Karidia Traoré, J. Biodiv. & Environ. Sci. 27(3), 10-22, September 2025.

Extreme rainfall variability and trends in the district of Ouedeme, municipality of Glazoue (Benin)

Koumassi Dègla Hervé, J. Biodiv. & Environ. Sci. 27(3), 1-9, September 2025.

Heterosis breeding, general and specific combining ability and stability studies in pearl millet: Current trends

Ram Avtar, Krishan Pal, Kavita Rani, Rohit Kumar Tiwari, Mahendra Kumar Yadav, J. Biodiv. & Environ. Sci. 27(2), 117-124, August 2025.

Combining ability, heterosis and stability for yield and fibre quality traits in cotton: Breeding approaches and future prospects

Rohit Kumar Tiwari, Krishan Pal, R. P. Saharan, Ram Avtar, Mahendra Kumar Yadav, J. Biodiv. & Environ. Sci. 27(2), 109-116, August 2025.

Bridging the COPD awareness gap in marginalized populations: Findings from a multicentre study in Khalilabad, Sant Kabir Nagar, Uttar Pradesh, India

Anupam Pati Tripathi, Jigyasa Pandey, Sakshi Singh, Smita Pathak, Dinesh Chaudhary, Alfiya Mashii, Farheen Fatima, J. Biodiv. & Environ. Sci. 27(2), 97-108, August 2025.

Antioxidant and anti-inflammatory activity of Pleurotus citrinopileatus Singer and Pleurotus sajor-caju (Fr.) Singer

P. Maheswari, P. Madhanraj, V. Ambikapathy, P. Prakash, A. Panneerselvam, J. Biodiv. & Environ. Sci. 27(2), 90-96, August 2025.

Mangrove abundance, diversity, and productivity in effluent-rich estuarine portion of Butuanon River, Mandaue City, Cebu

John Michael B. Genterolizo, Miguelito A. Ruelan, Laarlyn N. Abalos, Kathleen Kay M. Buendia, J. Biodiv. & Environ. Sci. 27(2), 77-89, August 2025.

Cytogenetic and pathological investigations in maize × teosinte hybrids: Chromosome behaviour, spore identification, and inheritance of maydis leaf blight resistance

Krishan Pal, Ravi Kishan Soni, Devraj, Rohit Kumar Tiwari, Ram Avtar, J. Biodiv. & Environ. Sci. 27(2), 70-76, August 2025.