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Characterization and isolation and molecular study of newcastle virus (achieved in 2017 in Iraq)

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Research Paper 01/02/2019
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Characterization and isolation and molecular study of newcastle virus (achieved in 2017 in Iraq)

Ameen A.S. Al-Rawi
Int. J. Biosci.14( 2), 13-17, February 2019.
Certificate: IJB 2019 [Generate Certificate]
Key: ChickenNewcastlePigeonSequence

Abstract

Objective: This research was achieved to update the available data about Newcastle in Iraq, the research was done on chickens and pigeon. Samples were gathered from farms in Baghdad, and were collected from certain organs precisely (lungs, brain and trachea), these organs were suspended in a saline buffer solution (pH 7.2) and then centrifuged and incubated for 10 days and chilled for one day, and then tested to find the molecular configuration which were calculated with DNA STAR software and analyzing with PHYML software. The mean death time for chicken was 35 hours while it was 48 for pigeon, the limited F gene frequency  to chickens shows 88% to Newcastle  virus chicken china / Jilin / YS03, sequencing analysis program shows identity of 96% of the Russian pigeon, and shows individuality of 89% to china chickens,  whereas Iraqi chickens show individuality of 85%. The differences in results could be related to differences in antigenic and genetic among the strains vaccine, and the flow strains fields.

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