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A comparative study of total phenolic contents and antioxidant potential of seeds of Peganum harmala

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Research Paper 01/03/2019
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A comparative study of total phenolic contents and antioxidant potential of seeds of Peganum harmala

Peganum harmala L., Methanol, Dichloromethane, Extraction, Total phenolic content, Antioxidant activity.
Int. J. Biosci.14( 3), 416-422, March 2019.
Certificate: IJB 2019 [Generate Certificate]
Key: Antioxidant activityDichloromethaneExtractionMethanolPeganum harmala L.Total Phenolic content

Abstract

Various solvents including methanol, hexane, benzene, chloroform and dichloromethane were utilized for the extraction of phenolic contents from the seeds of pegnum harmala. Total phenolic contents (TPC) were determined by using Folin-Ciocalteu reagent method against gallic acid as standard by UV-Vis spectrophotometer at 765 nm while DPPH  free radical scavenging activity of the extracts was measure by using UV-Vis spectrophotometer and taking reading at 517 nm and ascorbic acid as standard. Total phenolic contents 27.7 mg GA/g, 22.2 mg GA/g, 26.4 mg GA /g, 30.7 mg GA /g and 17.3 mg GA /g were for dichloromethane, benzene, chloroform, methanol and hexane extracts respectively. Methanol extract showed high TPC content and high antioxidant activity (72%) followed by dichloromethane extract (67%), chloroform extract (63 %), benzene extract (52 %) and hexane extract (48 %) respectively for 50 μL of each sample. The antioxidant activity was concentrated dependent for all the solvents and high TPC contents showed higher antioxidant activity.

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