An efficient DNA extraction protocol for medicinal plants
Paper Details
An efficient DNA extraction protocol for medicinal plants
Abstract
This paper communicates a simple and cost effective protocol for isolation of genomic DNA from dry parts of Berberis and Mentha. This protocol was applied to 5 species of Berberis and 4 species of Mentha collected from different locality of Kunhar Valley. In this protocol 5 M NaCl, 2% CTAB, 1% PVP and 0.1 % ß-mercaptoethanol were used and incubated at 600C for 25 minutes. Pure DNA extracted by this method was found sufficient and suitable for PCR amplification and Southern blot hybridization analyses, which are the important steps in crop improvement programme through marker development and genetic engineering techniques.
Aljanabi SM, Forget L, Dookun A. 1999. An improved rapid protocol for the isolation of polysaccharide and polyphenol–free sugarcane DNA. Plant Molecular Biology Reporter 17, 1–8. http://dx.doi.org/10.1023/A:1007692929505
Barzegari A, Vahed SZ, Atashpaz S, Khani S, Omidi Y. 2010. Rapid and simple methodology for isolation of high quality genomic DNA from coniferous tissues (Taxusbaccata). Molecular Biology Reports 37, 833–837. http://dx.doi.org/10.1007/s11033-009-9634-z
Clark MS. 1997. In: Plant Molecular Biology – A Laboratory Manual, p. 305–28, Springer–Verlog Berlin Heidelberg, New York.
Dellaporta SL, Wood J, Hicks JB. 1983. A plant DNA minipreparation: version II. Plant Molecular Biology Reporter 1, 19-21. http://dx.doi.org/10.1007/BF02712670
Doyle JJ, Doyle JL. 1990. Isolation of plant DNA from fresh tissue. Focus 12, 13-15.
Fang G, Hammar S, Rebecca R. 1992. A quick and inexpensive method for removing polysaccharides from plant genomic DNA. Biotechniques 13, 52-56.
Hedge IC. 1990. Labiatae; in S. I. Ali and Y. J. Nasir (edt.) Flora of Pakistan. Department of Botany, Karachi University, Karachi. Pakistan 192, 255-264.
Howland DE, Oliver RP, Davy AJ. 1991. A method of extraction of DNA from birch. Plant Molecular Biology Reporter 9, 340-344. http://dx.doi.org/10.1007/BF02672010
Jafri SMH. 1975. Berberidaceae. In E. Nasir and S. I. Ali (edt.) Flora of West Pakistan. Department of Botany, Karachi University, Karachi. Pakistan 87, 1-31.
Keim P, Olson TC, Shoemaker RC. 1988. A rapid protocol for isolating soybean DNA. Soybean Genet News 15, 150-152.
Khan S, Qureshi MI, Kamaluddin, Alam T, Abdin MZ. 2007. Protocol for isolation of genomic DNA from dry and fresh roots of medicinal plants suitable for RAPD and restriction digestion. African Journal of Biotechnology 6, 175-178.
Suman PS, Khanuja, Shasany AK, Darokar MP, Kumar S. 1999. Rapid isolation of DNA from dry and fresh samples of plants producing large amounts of secondary metabolites and essential oils. Plant molecular biology Reporter 17, 1 – 7. http://dx.doi.org/10.1023/A:1007528101452
Kumar A, Pushpangadan P, Mehrotra S. 2003. Extraction of high molecular weight DNA from dry root tissue of Berberis lycium suitable for RAPD. Plant Molecular Biology Reporter 21, 309a-309d. http://dx.doi.org/10.1007/BF02772807
Lodhi AM, Guang-Ning Ye, Norman F, Weeden, Bruce IR. 1994. A simple and efficient method for DNA extraction from grapevine cultivars, Vitis species and Ampelopsis. Plant Molecular Biology Reporter 12, 6-13. http://dx.doi.org/10.1007/BF02668658
Loomis MD. 1974. Overcoming problems of phenolics and quinones in the isolation of plant enzymes and organelles. Methods Enzymol 31, 528– 544.
Paterson AH, Brubaker CL, Wendel JF. 1993. A rapid method for extraction of Cotton (Gossypium spp) genomic DNA suitable for RFLP or PCR analysis. Plant Molecular Biology Reporter 11, 122–7. http://dx.doi.org/10.1007/BF02670470
Porebski S, Bailey LG, Baum BR. 1997. Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Molecular Biology Reporter 15, 8– 15. http://dx.doi.org/10.1007/BF02772108
Puchooa D, Venkatasamy K. 2005. A Protocol for the isolation of DNA from Trochetia boutoniana, International Journal of Agriculture & Biology 07, 82–85.
Sharma AD, Kaur GP, Prabhjeet S. 2002. DNA isolation from dry and fresh samples of polysaccharide-rich plants. Plant Molecular Biology Reporter 20, 415a-415f. http://dx.doi.org/10.1007/BF02772129
Deshmukh VP, Thakare PV, Choudhari US, Gawande PA. 2007. A simple method for isolation of genomic DNA from fresh and dry leaves of Terminalia arjuna (Roxb.) Wight and Arnot. Electronic Journal of Biotechnology 10, 468-472. http://dx.doi.org/10.2225/vol10-issue3-fulltext-5
Weising K, Nybom H, Wolff K, Meyer W. 1995. DNA isolation and purification. In: DNA fingerprinting in plants and fungi. CRC Press, Boca Raton, Florida 44–59.
Ajmal Iqbal, Israr Ahmad, Habib Ahmad, M. S. Nadeem, Mohammad Nisar, Huma Riaz (2013), An efficient DNA extraction protocol for medicinal plants; IJB, V3, N7, July, P30-35
https://innspub.net/an-efficient-dna-extraction-protocol-for-medicinal-plants/
Copyright © 2013
By Authors and International
Network for Natural Sciences
(INNSPUB) https://innspub.net
This article is published under the terms of the
Creative Commons Attribution License 4.0