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Antibacterial activity against S. agalactiae of four essential oils of peppermint (Mentha piperita L.), lemongrass (Cymbopogon citratus), garlic (Allium sativum), and green tea (Camellia sinensis)

Huyen Nguyen Thi Thuong, Thi-Hieu Tran, Phuong Nguyen Ngoc, Van-Thanh Vo

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Int. J. Biosci.21(6), 76-82, December 2022

DOI: http://dx.doi.org/10.12692/ijb/21.6.76-82


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The antibacterial activity test of four essential oils, including peppermint (Mentha piperita), lemongrass (Cymbopogon citratus), garlic (Allium sativum), and green tea (Camellia sinensis) at 100% concentration, was conducted by agar well diffusion method. The results of peppermint EO showed the largest zone of inhibition (23.00±3.93 mm), and S. agalactiae was considered susceptible. The following result is lemongrass EO (19.50±2.66 mm) showed tested organism was intermediately inhibited but was nonsusceptible with garlic EO (ZOI=12.50±2.48 mm) and green tea EO (ZOI=9.00±2.19 mm). Then, the experiment was performed to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The results recorded the MIC increasing gradually as follows: lemongrass EO (0.6%) < peppermint EO (0.75%) < garlic EO (1.75%) < green tea EO (4.5%). Similarly, the MBC value was lemon grass EO (1.0%) < peppermint EO (1.75%) < garlic EO (1.75%), and non-identified MBC of green tea EO. The difference in MIC/MBC along with ZOI between peppermint and lemongrass is believed to depend on the degree of diffusion of essential oil into agar medium and how the compounds affect bacterial cells.


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Antibacterial activity against S. agalactiae of four essential oils of peppermint (Mentha piperita L.), lemongrass (Cymbopogon citratus), garlic (Allium sativum), and green tea (Camellia sinensis)

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