Antibacterial activity of selected medicinal plants extracts against Escherichia coli isolates causing urinary tract infections
Paper Details
Antibacterial activity of selected medicinal plants extracts against Escherichia coli isolates causing urinary tract infections
Abstract
Antibiotic resistance was firstly observed soon after Alexander Fleming discovered penicillin in 1928. Resistance to commonly used antibiotics is one of the most threatening issues to public health across the globe. There has been an increasing demand to search for novel antimicrobial agents to combat the growing resistance. Medicinal plants are a rich source of many drugs especially antimicrobial drugs and these plants have been used from years to treat different types of diseases. The main goal of our study was evaluation of antimicrobial activity of the selected plant extracts against clinical isolates and then to compare resistance level of bacteria to commonly used antibiotics and medicinal plants. Methanolic extract was taken and dried in water bath; dried extracts were dissolved in Dimethyl Sulfoxide (DMSO) in different concentration (25mg/mL, 50mg/mL, and 100mg/mL). The zones of inhibition were measured by using agar well diffusion method. Methanolic Extracts of Paganum harmala, Fogonia cretica, Ajuga bracteosa and Olea feroogenia showed varying level of activities against E. coli. The results of the study shows that the antibacterial effect of selected plant extracts increased with increase in concentration. The extracts also showed activity against those isolates, which shows resistance to commonly used antibiotics. Therefore, it is suggested that antibacterial agents present in these plants should be isolated through advanced biochemical techniques such as HPLC for the evaluation of potency. These compounds may be evaluated as potential antimicrobial therapeutic agents against a wide range of human pathogens.
Ahmed A. 2015. Phytochemical analysis of selected medicinal plants for antidiabetic compounds, Arid Agriculture University, Rawalpindi Pakistan.
Alekshun MN, Levy SB. 2007. Molecular mechanisms of antibacterial multidrug resistance. Cell 128, 1037-1050.
Almasirad A, Hosseini R, Jalalizadeh H, Rahimi-Moghaddam Z, Abaeian N, Janafrooz M, Abbaspour M, Ziaee V, Dalvandi A, Shafiee A. 2006. Synthesis and analgesic activity of 2-phenoxybenzoic acid and N-phenylanthranilic acid hydrazides. Biological and Pharmaceutical Bulletin 29, 1180-1185.
Arshad N, Neubauer C, Hasnain S, Hess M. 2008. Peganum harmala can minimize Escherichia coli infection in poultry, but long-term feeding may induce side effects. Poultry science 87, 240-249.
Chopra I, Roberts M. 2001. Tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology of bacterial resistance. Microbiology and molecular biology reviews 65, 232-260.
Coenen S, Ferech M, Haaijer-Ruskamp FM, Butler CC, Vander Stichele RH, Verheij TJ, Monnet DL, Little P, Goossens H. 2007. European Surveillance of Antimicrobial Consumption (ESAC): quality indicators for outpatient antibiotic use in Europe. BMJ Quality & Safety 16, 440-445.
Graham J, Quinn M, Fabricant D, Farnsworth N. 2000. Plants used against cancer–an extension of the work of Jonathan Hartwell. Journal of ethnopharmacology 73, 347-377.
Hayacibara MF, Koo H, Rosalen PL, Duarte S, Franco EM, Bowen WH, Ikegaki M, Cury JA. 2005. In vitro and in vivo effects of isolated fractions of Brazilian propolis on caries development. Journal of ethnopharmacology 101,110-115.
Hoge CW, Gambel JM, Srijan A, Pitarangsi C, Echeverria P. 1998. Trends in antibiotic resistance among diarrheal pathogens isolated in Thailand over 15 years. Clinical infectious diseases 26, 341-345.
Israili ZH, Lyoussi B. 2009. Ethnopharmacology of the plants of genus Ajuga. Pakistan Journal of Pharmaceutical Sciences 22, 4.
Jaradat N. 2005. Ethnopharmacological survey of natural products in palestine.
Kaithwas G, Majumdar DK. 2012. In vitro antioxidant and in vivo antidiabetic, antihyperlipidemic activity of linseed oil against streptozotocin‐induced toxicity in albino rats. European Journal of Lipid Science and Technology 114, 1237-1245.
Khan MA, Prakash R, Ali S, Aljarbou A, Khan MA. 2011. Comparative study of antibacterial activity and toxicity of certain plants used in Unani medicine. Advances in Bioresearch 2, 10-13.
Kirbağ S, Zengin F, Kursat M. 2009. Antimicrobial activities of extracts of some plants. Pak. J. Bot 41, 2067-2070.
La Lastra C, Barranco M, Motilva V, Herrerias J. 2001. Mediterrranean diet and health biological importance of olive oil. Current pharmaceutical design 7, 933-950.
Madhuri S, Pandey G. 2009. Some anticancer medicinal plants of foreign origin. Current science 779-783.
Mehmood A, Murtaza G. 2018. Phenolic contents, antimicrobial and antioxidant activity of Olea ferruginea Royle (Oleaceae). BMC complementary and alternative medicine 18, 1-6.
Mikaili P, Maadirad S, Moloudizargari M, Aghajanshakeri S, Sarahroodi S. 2013. “Therapeutic uses and pharmacological properties of garlic, shallot, and their biologically active compounds.” Iranian journal of basic medical sciences 16, 1031.
MM T. 2007. Tohfeh-al-Momenin Rahimi R, Shams Ardekani MR, Farjadmand F, editors. Tehran: Shahid Beheshti University of Medical Sciences 129.
Mohsenipour Z, Hassanshahian M. 2016. Antibacterial activity of Espand (Peganum harmala) alcoholic extracts against six pathogenic bacteria in planktonic and biofilm forms.
Nascimento GG, Locatelli J, Freitas PC, Silva GL. 2000. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Brazilian journal of microbiology 31, 247-256.
Pandey A, Chawdhry P. 2006. Propagation techniques and harvesting time on productivity and root quality of Withania somnifera. Journal of Tropical Medicinal Plants 7, 79.
Ranalli A, Contento S, Lucera L, Di Febo M, Marchegiani D, Di Fonzo V. 2006. Factors affecting the contents of iridoid oleuropein in olive leaves (Olea europaea L.). Journal of agricultural and food chemistry 54, 434-440.
Sajid B, Alia E, Rizwana K, Uzma S, Alamgeer HM. 2011. Phytochemical screening and antimicrobial activity of Fagonia cretica plant extracts against selected microbes. J Pharm Res 4, 962-963.
Savarese M, De Marco E, Sacchi R. 2007. Characterization of phenolic extracts from olives (Olea europaea cv. Pisciottana) by electrospray ionization mass spectrometry. Food Chemistry 105, 761-770.
Setif A. 2011. Antibacterial activity of extract of Ajuga iva and Teucrium polium. Adv Environ Bio 52, 491-495.
Shad AA, Zeeshan M, Fazal H, Shah HU, Ahmed S, Abeer H, Abd_Allah E, Ullah R, Afridi H, Asma MA. 2016. Physio-chemical evaluation and biological activity of Ajuga bracteosa wall and Viola odoroto Linn. African Journal of Traditional, Complementary and Alternative Medicines 13, 40-46.
Shakeri A, Hazeri N, Vlizadeh J, Ghasemi A, Tavallaei FZ. 2012. Phytochemical screening, antimicrobial and antioxidant activities of Anabasis aphylla L. extracts. Kragujevac Journal of Science 34, 71-78.
Shariff ZU. 2001. Modern herbal therapy for common ailments, Spectrum Books.
Shen X, Isogai A, Furihata K, Sun H, Suzuki A. 1993. Two neo-clerodane diterpenoids from Ajuga macrosperma. Phytochemistry 33, 887-889.
Shinwari, ZK. 2010. Medicinal plants research in Pakistan. Journal of medicinal plants research 4,161-176.
Muhammad Junaid Khan, Asmat Ullah, Uroosa Naseem, Inam Ullah, Nadeem Ullah, Muhammad Sameer ishaq, Mohammad Nasar, Basit Ali Khan, Shakeel Ahmad, Muhammad Zahid, Nadia Mubarik (2021), Antibacterial activity of selected medicinal plants extracts against Escherichia coli isolates causing urinary tract infections; IJMM, V13, N1, July, P9-17
https://innspub.net/antibacterial-activity-of-selected-medicinal-plants-extracts-against-escherichia-coli-isolates-causing-urinary-tract-infections/
Copyright © 2021
By Authors and International
Network for Natural Sciences
(INNSPUB) https://innspub.net
This article is published under the terms of the
Creative Commons Attribution License 4.0