Antibiotic susceptibility profiling and in-vitro antibacterial activity of some plant extracts to Escherichia coli isolated from spoiled rice and egg

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Research Paper 01/05/2012
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Antibiotic susceptibility profiling and in-vitro antibacterial activity of some plant extracts to Escherichia coli isolated from spoiled rice and egg

M. Javed Foysal, M. Nazmul Hossain, Fahmida Yeasmin, Shamsul Haq Miyan, M. Mahbubur Rahman
Int. J. Biosci.2( 5), 40-46, May 2012.
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Abstract

Studies were conducted to isolate and identify Escherichia coli from deteriorated rice and eggs as well as to evaluate antibiotic sensitivity pattern and screen some herbal extracts for their antibacterial activity against E. coli. A total of 10 from 52 isolates were identified by a series of morphological, physiological and biochemical tests. Eight antibiotics were tested by disc diffusion method where 90% of E. coli isolates showed resistance against sulphamethoxazole but found to be sensitive against gentamicin, ciprofloxacin and tetracycline. Erythromycin, streptomycin and cephradin showed 20% resistance in their sensitivity test results. However some medicinal plant extracts have been investigated during the study to assay their antibacterial activity against E. coli isolates so that diarrhea and dysentery associated with these bacteria can be treated by those fastidious herbs. Among 32 herbal extracts tested 12 extracts showed antibacterial activity against E. coli. Leaf extracts of Temarindus indicus, Terminalia chebula, Citrus aurantifolia, Eugenia caryophyllus, Spondius pinnata and bulb extracts of Raphanus sativus were able to exhibit antibacterialactivity against all Escherichia Coli isolates. Thus medicinal herbs would be the better solution for the effective treatment of E. coli associated diarrhea and dysentery.

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Alam M, Rahman MM, Foysal MJ, Hossain MN. 2011. Determination of lethal concentration and antimicrobial activity of commonly used disinfectants. International Journal of Natural Science 1, 102-105.

Arunsri JR, Chanapong RW. 2009. Antibacterial effect of crude alcoholic and aqueous extracts of six medicinal plants against Staphylococcus aureus and Escherichia coli. Journal of Health Research 23, 153-156.

David IE and Royston GA. 2001. Rapid and quantitative detection of the microbial spoilage of muscle foods: current status and future trends. Trends in Food Science & Technology 12, 414–424.

Doyle EM. 2007. Microbial Food Spoilage — Losses and Control Strategies: A Brief Review of the Literature, Food Research Institute Briefings, University of Wisconsin–Madison, 1-16.

Ferdowsy H, Foysal MJ, Hossain, MN, Rahman MM. 2011. Isolation of Edwardsiella sp. from diseased catfish and their sensitivity to some antibiotics and medicinal plant extracts. International Journal of Bio-Research 11, 48-53.

Metta O, Foysal MJ, Rahman MM, Alam M. 2011. Antibiotic Sensitivity and in vitro antimicrobial activity of plant extracts to Pseudomonas fluorescens isolates collected from diseased fish. International Journal of Natural Science 1, 82-88.

Hasan M. 2010. A comparative study on the antibacterial activity of herbal extracts with commonly used antibiotics to UTI causing E. coli clinical isolates. B.Sc thesis, Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh, 56 pages.

John DB, Robert CC. 1960. The spread plate as a method for the enumeration of marine bacteria. Department of Bacteriology, University of Connecticut 5, 78-80.

Khunt M, Pandhi N. 2011. Moderate halophilic bacterial community in excreta of wild ass (Equus hemionus khur). International Journal of Biosciences 1, 31-37.

Lakhotia RL, Stephens JF. 1973. Drug resistance and R factors among enterobacteria isolated from eggs. Poultry Science 52, 1955-1962.

Ongsakul M, Arunsri JR, Chanapong RW. 2009. Antibacterial effect of crude alcoholic and aqueous extracts of six medicinal plants against Staphylococcus aureus and escherichia coli. Journal of Health Research 23, 153-156.

Pragada MP, Rao SD, Venkaiah M. 2012. Study of some ethnomedicinal plants for treatment of dysentery of North Coastal Andhra Pradesh, India. International Journal of Biosciences 2, 18-24.

Rahman MM, Ferdowsy H, Kashem MA, Foysal MJ. 2010. Tail and fin rot disease of carp and climbing perch in Bangladesh. Journal of Biological Sciences 10, 800- 804.

Rahman MM, Hossain MN. 2010. Antibiotic and herbal sensitivity of some Aeromonas sp. collected from diseased carp fish. Progressive Agriculture 21, 117-129.

Rahman  SM,  Doha  SKM,  Rahman  R.  2011. Antidiarrhoeal activity of the leaf and fruit extracts of Dillenia indica. International Journal of Biosciences 1, 39-46.

Shan B, Zhong CY, John DB, Harold CK. 2007. The in vitro antibacterial activity of dietary spice and medicinal herb extracts. International Journal of Food Microbiology 117, 112–119.