Antioxidant, antimicrobial activity and mineral profile of Moringa oleifera leaves extracts

Paper Details

Research Paper 01/01/2018
Views (278) Download (5)
current_issue_feature_image
publication_file

Antioxidant, antimicrobial activity and mineral profile of Moringa oleifera leaves extracts

Muhammad Asif, Sajjad Hussain, Taj Ur Rahman, Sadaf Mutahir, Muhammad Aurang Zeb
Int. J. Biosci.12( 1), 356-361, January 2018.
Certificate: IJB 2018 [Generate Certificate]

Abstract

This study was shaped to discover the antimicrobial, antioxidant and mineral profile of Moringa oleifera leaves extracts. The leaves crude extract was fractionated into different fractions using solvents of varying polarity. DPPH and FRAP methods were used to evaluate the antioxidant capacities of the extract fractions. DPPH scavenging activities were found in the range of 44.6 to 62.04% whereas the reducing power was in the range of 1.4 to 1.85mg/ml for ether and methanolic extracts respectively. Total phenolic contents (TPC) and total flavonoid contents (TFC) were also calculated and were found 284.66 ppm and 85.12 for methanolic and ethanolic extracts respectively. Antimicrobial analysis was completed against two fungal strains Aspergillus niger and Aspergillus flavus and bacterial strains Escherichia coli and Bacillus subtilis using Rifampicin and Terbinafine as control. ZID and MIC values were calculated. The analysis of mineral profile showed rich amounts of important minerals, in which the value for potassium was found to be greatest (17.12 ppm). The results of antioxidant and antimicrobial assays revealed that the plant leaves exhibited potent use to sustain healthy life.

VIEWS 5

Bridges JW, Benford DJ, Hubband SA. 1993. The mechanism of toxic Injury. Annals. New York Academy of Sciences 83, 42-63.

Dekkers JC, Vandoornen LJP, Kemper CJ. 1996. The Role of Antioxidant Vitamins and Enzymes in the Prevention of Exercise- Induced Muscle Damage. Sports Medicine 21, 213-238.

Mahmoudi M, Ebrahimzadeh MA, Ansaroudi F, Nabavi SF, Nabavi SM. 2009. Antidepresswnt and antioxidant activities of Artemisia absinthium L. at flowering stage. African journal of Biotechnology 8, 7170-7175.

Manzoor M, Anwar F, Iqba TI, Bhnager MI. 2007. Physico-chemical characterization of Moringa concanensis seeds and seed oil. J. Am. Oil Chem. Soc 84, 413-419.

Mishra SP, Singh P, Singh S. 2011. Nutritional and medicinal value of Moringa oleifera leaves: Potential and Prospects. Forestry Bulletin 11, 46-58.

Morton JF. 1991. “The horseradish tree, Moringa pterygosperma (Moringaceae)-A boon to Arid Lands?” Economic Botany 45, 318-333.

Palada MC. 1996. “Moringa (Moringa oleifera Lam.): a versatile tree crop with horticultural potential in the subtropical United States,” HortScience 31, 794-797.

Patra JK, Rath SK, Jena K, Rathod VK, Hrudayanath T. 2008. Evaluation of antioxidant and antimicrobial activity of seaweed (Sargassum sp.) extract: A study on inhibition of glutathione-s-transferase activity. Turkish Journal Biology 32, 119-125.

Shanab MMS. 2007. Antioxidant and antibiotic activities of some seaweeds (Egyptian isolates). International Journal of Agricultural Biologz 9, 220-225.

Singleton VL, Rossi JA. 1965. Colorimetry of total phenolic with phosphomolybdic phosphotungstic acid reagents. American Journal of Enology and Viticulture 16, 144-153.

Zhishen J, Mengcheng T, Jianming W. 1999. The determination of flavonoid Contents of mulberry and their scavenging effects on superoxide radicals. Food Chemistry 64, 555-559.