Asessment of antibacterial activity of bacteria immunized Muga silkworm (Antheraea assamensis Helfer) and its comparison with market antibiotics
Paper Details
Asessment of antibacterial activity of bacteria immunized Muga silkworm (Antheraea assamensis Helfer) and its comparison with market antibiotics
Abstract
The Muga silkworm, Antheraea assamensis Helfer, which plays a huge role in the socio-economic scenario of Assam is susceptible to a wide range of infections due to the outdoor rearing of these silkworms. Infection or injury triggers the immune response of their body, which involves several components including the production of some antimicrobial proteins for defense against the invading microorganism. The current study deals with the immunization of the Muga silkworms with Bacillus thuringiensis to observe the response of the activated haemolymph against E. coli and B. thuringiensis. Analysis was carried out to compare the antibacterial activity of the immunized haemolymph with that of three common market antibiotics, Levofloxacin, Azithromycin and Rifaximin. After immunization, haemolymph was procured at different time intervals i,e 6 hours, 12 hours, 18 hours and 24 hours and each time interval was taken as a different group along with one control group. Analysis for total protein and free amino acid for all groups were carried out which showed increase in protein and free amino acid concentration in all groups as compared to the control haemolymph samples. No antimicrobial action was shown by the samples obtained at 6 hours, 12 hours and 18 hours with only the immunized haemolymph obtained after 24 hours showed positive activity against both B. thuringiensis as well as E.coli along with potency almost at par with the antibiotics taken for the study. Thus, the study revealed clear roles of bacteria immunized haemolymph to fight against infections and proper characterization of the components of the immunized haemolymph might lead to more specific understanding of such immune mechanisms as well as their role as future antibiotics.
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Shibani Kalita, Tanushree Biswas, Ankita Devi , Sanghamitra Saharia, Dimpimoni Kalita, Sunayan Bardoloi (2023), Asessment of antibacterial activity of bacteria immunized Muga silkworm (Antheraea assamensis Helfer) and its comparison with market antibiotics; IJB, V23, N2, August, P220-227
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