Assessment of oxidative stress tolerance in red bean (Phaseolus vulgaris L.) seedling under salinity

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Research Paper 01/12/2014
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Assessment of oxidative stress tolerance in red bean (Phaseolus vulgaris L.) seedling under salinity

Sajjad Moharramnejad, Mostafa Valizadeh
Int. J. Agron. Agri. Res.5( 6), 49-56, December 2014.
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In order to evaluate salt tolerance in red bean (Phaseolus vulgaris L.) 10 genotypes were exposed to two levels of NaCl i.e., 0 and 400 mM in laboratory conditions. Plant fresh weight, total phenolics, total soluble proteins, hydrogen peroxide (H2O2) and malondialdehyde (MDA) in leaves salt-stressed and non-stressed plants were analyzed. Electrophoretic analyses were performed by using 8% slab polyacrylamide gels. Superoxide dismutase (SOD), peroxidase (POX) and catalase (CAT) were stained and for each isozymic band the “density × area” scores onto gels were evaluated by MCID software as enzymatic activity. The salt stress reduced fresh weight of red bean genotypes. MDA, H2O2, total soluble proteins and total phenolics were significantly elevated in salinity condition. Salt stress increased activities of SOD, POX3 and CAT in all red bean genotypes. These results seem to indicate that 31126 genotype of red bean tolerance to salt stress is associated with enhance activity of antioxidant enzymes. Different antioxidant enzymes and other characters analyzed, only SOD2 isozyme activity was found to be associate with salt tolerance in red bean genotypes examined.


Ashraf M. 2009. Biotechnological approach of improving plant salt tolerance using antioxidants as markers. Biotechnology Advances 27, 84–93.

Asish KP, Anath BD, Prasanna M. 2004. Defense potentials to NaCl in mangrove, Bruguiera parviflora: Differential changes of isoforms of some antioxidant enzymes. Journal of Plant Physiology 161, 531-542.

Bayuelo-Jimenez JS, Debouck DG, Lynch JP. 2002. Salinity tolerance in Phaseolus species during early vegetative growth. Crop Science 42, 2184-2192.

Bradford MM. 1976. A rapid and sensitive method for the quantitation of micro gram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–54.

Chinta S, Lakshmi A, Giridarakumar S. 2001. Change in the antioxidant enzyme efficacy in two high yielding genotypes of mulberry (Morus alba L.) under NaCl salinity. Plant Science 161, 613-619.

Diego AM, Marco AO, Carlos AM, José C. 2003. Photosynthesis and activity of superoxide dismutase peroxidase and glutathione reductase in cotton under salt stress. Environmental and Experimental Botany 49, 69-76.

Dogan M. 2012. Investigation of the effect of salt stress on the antioxidant enzyme activities on the young and old leaves of salsola (Stenopera) and tomato (Lycopersicon esculentum L.). African Journal of Plant Science 6, 62-72.

Comba ME, Benavides MP, Gallego SM, Tomaro ML. 1998. Relationship between nitrogen fixation and oxidative stress induction in nodules of salt-treated soybean plants. Environmental and Experimental Botany 60,155-126.

Gaber MA. 2010. Antioxidative defense under salt stress. Plant Signaling and Behavior 5, 369-374.

Lachaal M, Grignon C, Hajji M. 2002. Growth rate affects salt sensitivity in two lentil populations. Journal of Plant Nutrition. 25, 2613-2625.

Mittal R, Dubey RS. 1991. Behaviour of peroxidases in rice: changes in enzyme activity and isoforms in relation to salt tolerance. Plant Physiology and Biochemistry 29, 31-40.

Munns R. 2002. Comparative physiology of salt and water stress. Plant Cell and Environment 25, 239–250.

Nagesh Babu R, Devaraj VR. 2008. High temperature and salt stress response in French bean (Phaseolus vulgaris). Australian Journal of Crop Science 2, 40-48.

Noreen Z, Ashraf M. 2009. Assessment of variation in antioxidative defense system in salt-treated pea (Pisum sativum) cultivars and its putative use as salinity tolerance markers. Journal of Plant Physiology 166, 1764-1774.

Tijen D, Ismail T. 2005. Comparative lipid peroxidation, antioxidant defense systems and praline content in roots of two rice cultivar differing in salt tolerance. . Environmental and Experimental Botany 53, 143-151.

Valizadeh M, Moharamnejad S, Ahmadi M, Mohammadzadeh Jalaly H. 2013. Changes in activity profile of some antioxidant enzymes in alfalfa half-sib families under salt stress Journal of Agricultural Science and Technology 15, 801-809.

Velikova V, Yordanov I, Adreva A. 2000. Oxidative stress and some antioxidant systems in acid rain treated bean plants; protective role of exogenous polyamines. Plant Science 151, 59–66.

Wahid A, Ghazanfar A. 2006. Possible involvement of some secondary metabolites in salt tolerance of sugarcane. Journal of Plant Physiology 163, 723–730.

Wang X, Han J. 2009. Changes of proline content, activity, and active isoforms of antioxidative enzymes in two alfalfa cultivars under salt stress. Agricultural Sciences in China 8, 431-440.

Wang X, Zhao G, Gu H. 2009. Physiological and antioxidant responses of three Leguminous species to saline Environment during seed germination stage. African Journal of Biotechnology 8, 5773-5779.

Wang W, Kim Y, Lee H, Kim K, Deng X, Kwak S. 2009. Analysis of antioxidant enzyme activity during germination of alfalfa under salt and drought stresses. Plant Physiology and Biochemistry 47, 570–577.