Bacteriological profile and antibiogram of urinary tract infections in a Tertiary care Hospital

Abstract

Urinary tract infections are amongst the most common cause of bacterial infections in humans. As there is an emergence of resistance in the uropathogens to multiple drugs, a present study was conducted to study the uropathogens and their antibiotic sensitivity pattern. The present study was conducted to isolate and identify the bacterial pathogens causing urinary tract infections and their antibiotic sensitivity pattern. The study was conducted in the Department of Microbiology and urine samples sent for culture and sensitivity over a period of 6 months from January 2020 to June 2020 were included in the study. Samples were collected, transported and processed in the laboratory as per the standard protocol. Antibiotic sensitivity test was done by Kirby Bauer disc diffusion method. Out of 285 samples, 158 (55.43%) yielded growth and 127 (44.56%) yielded no growth. Among the culture positive cases, Females 105 (66.5%) were more affected than males 53 (33.5%). The most predominant organism isolated was Escherichia coli 68(43.03%) followed by Klebsiella pneumoniae 29 (18.35%), Coagulase Negative Staphylococci 20(12.65%), Pseudomonas aeroginosa 18 (11.39%). Among the Gram positive cocci, CoNS 20 (12.65%) was the predominant organism followed by Enterococcus species 13 (8.3%) and Staphylococcus aureus 4 (2.3%). Escherichia coli were sensitive to Nitrofurantoin (100%), and Ceftriaxone (96%). Klebsiella pneumoniae was sensitive to Norfloxacin (90%), Ciprofloxacin (90%).Pseudomonas aeroginosa was sensitive to Cotrimoxazole (96%), Norfloxacin (90%). CoNS were sensitive to Norfloxacin (100%), Levofloxacin (100%), Vancomycin (100%). The present study concludes that among the urinary tract infections cases. Females were more affected than males. The predominant organism isolated was Escherichia coli and sensitive to Nitrofurantoin, Ceftriaxone. Among the Gram positive cocci CoNS was frequently isolated and sensitive to Levofloxacin, Vancomycin and Norfloxacin.